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Literature summary for 3.2.1.147 extracted from

  • Loebers, A.; Mueller-Uri, F.; Kreis, W.
    A young root-specific gene (ArMY2) from horseradish encoding a MYR II myrosinase with kinetic preference for the root-specific glucosinolate gluconasturtiin (2014), Phytochemistry, 99, 26-35.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
phylogenetic tree, recombinant overexpression of His6-tagged ArMy2 in Pichia pastoris Armoracia rusticana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
gluconasturtiin recombinant enzyme, pH 5.5, 37°C Armoracia rusticana
0.53
-
sinigrin recombinant enzyme, pH 5.5, 37°C Armoracia rusticana
16.81
-
4-nitrophenyl beta-D-glucopyranoside recombinant enzyme, pH 5.5, 37°C Armoracia rusticana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
x * 57000, SDS-PAGE Armoracia rusticana

Organism

Organism UniProt Comment Textmining
Armoracia rusticana I1TIJ2 young root-specific gene ArMY2
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the enzyme ArMy2 possesses 5 putative N-glycosylation sites, degree of glycosylation is about 19% Armoracia rusticana

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged ArMy2 from Pichia pastoris by nickel affinity chromatography Armoracia rusticana

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Armoracia rusticana
-
additional information glucosinolate contents in young and storage root and in leaves, overview Armoracia rusticana
-
root
-
Armoracia rusticana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-glucopyranoside + H2O a poor substrate for ArMy2 Armoracia rusticana 4-nitrophenol + D-glucopyranose
-
?
glucobrassicin + H2O
-
Armoracia rusticana ?
-
?
gluconasturtiin + H2O
-
Armoracia rusticana ?
-
?
additional information myrosinase-catalyzed deglycosylation of a glucosinolate releases beta-D-glucose and an unstable intermediate, which undergoes rearrangements to finally yield an isothiocyanate Armoracia rusticana ?
-
?
sinigrin + H2O
-
Armoracia rusticana ?
-
?

Subunits

Subunits Comment Organism
? x * 57000, SDS-PAGE Armoracia rusticana

Synonyms

Synonyms Comment Organism
ArMy2
-
Armoracia rusticana
MYR II
-
Armoracia rusticana
myrosinase
-
Armoracia rusticana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
-
Armoracia rusticana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.21
-
4-nitrophenyl beta-D-glucopyranoside pH 5.5, 37°C Armoracia rusticana
8.91
-
gluconasturtiin pH 5.5, 37°C Armoracia rusticana
10.69
-
sinigrin pH 5.5, 37°C Armoracia rusticana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 5.5 rather sharp maximum between pH 5.0-5.5 Armoracia rusticana

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.26
-
4-nitrophenyl beta-D-glucopyranoside recombinant enzyme, pH 5.5, 37°C Armoracia rusticana
20.16
-
sinigrin recombinant enzyme, pH 5.5, 37°C Armoracia rusticana
83.13
-
gluconasturtiin recombinant enzyme, pH 5.5, 37°C Armoracia rusticana