Cloned (Comment) | Organism |
---|---|
phylogenetic tree, recombinant overexpression of His6-tagged ArMy2 in Pichia pastoris | Armoracia rusticana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
gluconasturtiin | recombinant enzyme, pH 5.5, 37°C | Armoracia rusticana | |
0.53 | - |
sinigrin | recombinant enzyme, pH 5.5, 37°C | Armoracia rusticana | |
16.81 | - |
4-nitrophenyl beta-D-glucopyranoside | recombinant enzyme, pH 5.5, 37°C | Armoracia rusticana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
x * 57000, SDS-PAGE | Armoracia rusticana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Armoracia rusticana | I1TIJ2 | young root-specific gene ArMY2 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the enzyme ArMy2 possesses 5 putative N-glycosylation sites, degree of glycosylation is about 19% | Armoracia rusticana |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged ArMy2 from Pichia pastoris by nickel affinity chromatography | Armoracia rusticana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Armoracia rusticana | - |
additional information | glucosinolate contents in young and storage root and in leaves, overview | Armoracia rusticana | - |
root | - |
Armoracia rusticana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-glucopyranoside + H2O | a poor substrate for ArMy2 | Armoracia rusticana | 4-nitrophenol + D-glucopyranose | - |
? | |
glucobrassicin + H2O | - |
Armoracia rusticana | ? | - |
? | |
gluconasturtiin + H2O | - |
Armoracia rusticana | ? | - |
? | |
additional information | myrosinase-catalyzed deglycosylation of a glucosinolate releases beta-D-glucose and an unstable intermediate, which undergoes rearrangements to finally yield an isothiocyanate | Armoracia rusticana | ? | - |
? | |
sinigrin + H2O | - |
Armoracia rusticana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 57000, SDS-PAGE | Armoracia rusticana |
Synonyms | Comment | Organism |
---|---|---|
ArMy2 | - |
Armoracia rusticana |
MYR II | - |
Armoracia rusticana |
myrosinase | - |
Armoracia rusticana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
- |
Armoracia rusticana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.21 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.5, 37°C | Armoracia rusticana | |
8.91 | - |
gluconasturtiin | pH 5.5, 37°C | Armoracia rusticana | |
10.69 | - |
sinigrin | pH 5.5, 37°C | Armoracia rusticana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 5.5 | rather sharp maximum between pH 5.0-5.5 | Armoracia rusticana |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.26 | - |
4-nitrophenyl beta-D-glucopyranoside | recombinant enzyme, pH 5.5, 37°C | Armoracia rusticana | |
20.16 | - |
sinigrin | recombinant enzyme, pH 5.5, 37°C | Armoracia rusticana | |
83.13 | - |
gluconasturtiin | recombinant enzyme, pH 5.5, 37°C | Armoracia rusticana |