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Literature summary for 3.2.1.147 extracted from

  • Bellostas, N.; Petersen, I.L.; Sorensen, J.C.; Sorensen, H.
    A fast and gentle method for the isolation of myrosinase complexes from Brassicaceous seeds (2007), J. Biochem. Biophys. Methods, 70, 918-925.
    View publication on PubMed
Search for more literature for 3.2.1.147

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
140000 200000 enzyme forms complexes of different molecular weight with several protein subunits Brassica napus
140000 200000 enzyme forms complexes of different molecular weight with several protein subunits Sinapis alba
270000 350000 enzyme forms complexes of different molecular weight with several protein subunits Brassica oleracea
500000 600000 enzyme forms complexes of different molecular weight with several protein subunits Brassica carinata

Organism

Organism UniProt Comment Textmining
Brassica carinata
-
-
-
Brassica napus
-
-
-
Brassica oleracea
-
-
-
Sinapis alba
-
-
-

Purification (Commentary)

Purification (Comment) Organism
fast and gentle procedure for hte isolation of enzyme complex from seed Brassica carinata
fast and gentle procedure for the isolation of enzyme complex from seed Brassica napus
fast and gentle procedure for the isolation of enzyme complex from seed Brassica oleracea
fast and gentle procedure for the isolation of enzyme complex from seed Sinapis alba

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
 Brassica napus
-
seed
-
 Brassica oleracea
-
seed
-
 Sinapis alba
-
seed
-
 Brassica carinata
-

Subunits

Subunits Comment Organism
? enzyme consists of complexes of several subunits with molecular weights between 10000 Da and 110000 Da, SDS-PAGE Brassica napus
? enzyme consists of complexes of several subunits with molecular weights between 10000 Da and 110000 Da, SDS-PAGE Brassica oleracea
? enzyme consists of complexes of several subunits with molecular weights between 10000 Da and 110000 Da, SDS-PAGE Sinapis alba
? enzyme consists of complexes of several subunits with molecular weights between 10000 Da and 110000 Da, SDS-PAGE Brassica carinata

pI Value

Organism Comment pI Value Maximum pI Value
Brassica napus enzyme complex is formed by isoenzymes with isoelectric points between 4.8 and 5.6 5.6 4.8
Brassica oleracea enzyme complex is formed by isoenzymes with isoelectric points between 4.8 and 5.6 5.6 4.8
Sinapis alba enzyme complex is formed by isoenzymes with isoelectric points between 4.8 and 5.6 5.6 4.8
Brassica carinata enzyme complex is formed by isoenzymes with isoelectric points between 4.8 and 5.6 5.6 4.8