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Literature summary for 3.2.1.147 extracted from
- The myrosinase-glucosinolate interaction mechanism studied using some synthetic competitive inhibitors (1996), FEBS Lett., 385, 87-90.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-deoxyglucotropaeolin | - |
Lepidium sativum |  |
| NaCl | 0.5 M, 30% inhibition, 2 M, complete inhibition | Lepidium sativum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.033 | - |
4-deoxyglucotropaeolin | - |
Lepidium sativum | |
| 0.068 | - |
6-deoxyglucotropaeolin | - |
Lepidium sativum | |
| 0.075 | - |
glucotropaeolin | - |
Lepidium sativum | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lepidium sativum | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| a thioglucoside + H2O = a sugar + a thiol | the mechanism involves a first step acid-base catalyzed reaction with the formation of the glycosyl enzyme intermediate and the benzyl isothiocyanate obtained by the enzyme-independent Lossen rearrangement of the transient thiohydroximate-O-sulfonate, a sulfate anion and a proton are also released | Lepidium sativum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | - |
Lepidium sativum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-deoxyglucotropaeolin + H2O | - |
Lepidium sativum | ? | - |
? | |
| 4-deoxyglucotropaeolin + H2O | - |
Lepidium sativum | ? | - |
? | |
| 6-deoxyglucotropaeolin + H2O | - |
Lepidium sativum | ? | - |
? | |
| glucotropaeolin + H2O | - |
Lepidium sativum | ? | - |
? | |
| sinigrin + H2O | - |
Lepidium sativum | D-glucose + 3-isothiocyanatoprop-1-ene + SO42- | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.6 | - |
3-deoxyglucotropaeolin | - |
Lepidium sativum | |
| 3.1 | - |
4-deoxyglucotropaeolin | - |
Lepidium sativum | |
| 9.4 | - |
6-deoxyglucotropaeolin | - |
Lepidium sativum | |
| 65.6 | - |
glucotropaeolin | - |
Lepidium sativum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Lepidium sativum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 8 | pH 3.0: about 45% of maximal activity, pH 8.0: about 55% of maximal activity | Lepidium sativum |
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