Literature summary for 3.2.1.147 extracted from

Chen, S.; Halkier, B.A.
Functional expression and characterization of the myrosinase MYR1 from Brassica napus in Saccharomyces cerevisiae (1999), Protein Expr. Purif., 17, 414-420.

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Activating Compound

Activating Compound	Comment	Organism	Structure
ascorbic acid	activates	Brassica napus

Cloned(Commentary)

Cloned (Comment)	Organism
expression of myrosinase MYR1 in Saccharomyces cerevisiae. The recombinant enzyme is enzymatically active which shows that the enzyme, which in plant is complex bound with myrosinase-binding proteins and myrosinase-associated proteins, is functional in its free form. Expression of the enzyme is transient and the growth of the yeast cells is significantly reduced during the period of expression of myrosinase	Brassica napus

Cloned (Comment)

Organism

expression of myrosinase MYR1 in Saccharomyces cerevisiae. The recombinant enzyme is enzymatically active which shows that the enzyme, which in plant is complex bound with myrosinase-binding proteins and myrosinase-associated proteins, is functional in its free form. Expression of the enzyme is transient and the growth of the yeast cells is significantly reduced during the period of expression of myrosinase

Brassica napus

Inhibitors

Inhibitors	Comment	Organism	Structure
2-deoxy-2-fluoroglucotropaeolin	-	Brassica napus
castanospermine	-	Brassica napus

Organism

Organism	UniProt	Comment	Textmining
Brassica napus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
p-hydroxybenzylglucosinolate + H2O	-	Brassica napus	?	-	?
sinigrin + H2O	-	Brassica napus	D-glucose + 3-isothiocyanatoprop-1-ene + SO42-	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
36	60	recombinant enzyme	Brassica napus

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
20	65	20°C: about 85% of maximal activity, 65°C: about 45% of maximal activity, recombinant enzyme	Brassica napus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	7	recombinant enzyme	Brassica napus

pH Range

pH Minimum	pH Maximum	Comment	Organism
4.5	9	pH 4.5: about 55% of maximal activity, pH 9.0: about 80% of maximal activity, recombinant enzyme	Brassica napus