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Literature summary for 3.2.1.147 extracted from

  • Shikita, M.; Fahey, J.W.; Golden, R.R.; Holtzclaw, W.D.; Talalay, P.
    An unusual case of uncompetitive activation' by ascorbic acid: purification and kinetic properties of a myrosinase from Raphanus sativus seedlings (1999), Biochem. J., 341, 725-732.
No PubMed abstract available
Search for more literature for 3.2.1.147

Activating Compound

Activating Compound Comment Organism Structure
ascorbic acid activates Raphanus sativus

Inhibitors

Inhibitors Comment Organism Structure
glucose very weak inhibition Raphanus sativus
SO42-
-
 Raphanus sativus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.023
-
 sinigrin in absence of ascorbate Raphanus sativus
0.25
-
 sinigrin in presence of 0.5 mM ascorbate Raphanus sativus
14
-
 p-nitrophenyl-beta-D-glucopyranoside in absence of ascorbate Raphanus sativus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
 1 * 61000 + 1 * 62000, SDS-PAGE Raphanus sativus
62000
-
 1 * 61000 + 1 * 62000, SDS-PAGE Raphanus sativus
120000
-
 gel filtration Raphanus sativus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Raphanus sativus the enzyme is normally physically segregated from the glucosinolates, but when the cells are damaged, e.g. during food preparation, mastication or injury by predators such as insects, the enzyme is released and catalyzes their hydrolysis of glucosinolates ?
-
 ?

Organism

Organism UniProt Comment Textmining
Raphanus sativus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Raphanus sativus

Source Tissue

Source Tissue Comment Organism Textmining
root
-
 Raphanus sativus
-
seedling
-
 Raphanus sativus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
164
-
-
 Raphanus sativus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is normally physically segregated from the glucosinolates, but when the cells are damaged, e.g. during food preparation, mastication or injury by predators such as insects, the enzyme is released and catalyzes their hydrolysis of glucosinolates Raphanus sativus ?
-
 ?
p-nitrophenyl beta-D-glucopyranoside + H2O
-
 Raphanus sativus ?
-
 ?
sinigrin + H2O
-
 Raphanus sativus D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 61000 + 1 * 62000, SDS-PAGE Raphanus sativus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.11
-
 sinigrin in absence of ascorbate Raphanus sativus
287
-
 sinigrin in presence of 0.5 mM ascorbate Raphanus sativus