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Literature summary for 3.2.1.147 extracted from

  • Ohtsuru, M.; Tsuruo, I.; Hata, T.
    Studies on fungous myrosinase. Part I. Production, purification and some characteristics (1969), Agric. Biol. Chem., 33, 1309-1314.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6
-
sinigrin
-
Aspergillus sydowii

Organism

Organism UniProt Comment Textmining
Aspergillus sydowii
-
IFO 4284
-

Purification (Commentary)

Purification (Comment) Organism
-
Aspergillus sydowii

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
Aspergillus sydowii
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.07
-
-
Aspergillus sydowii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sinigrin + H2O
-
Aspergillus sydowii D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
pH 7.0, 15 min, stable Aspergillus sydowii
50
-
pH 7.0, 15 min, complete loss of activity Aspergillus sydowii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Aspergillus sydowii

pH Range

pH Minimum pH Maximum Comment Organism
4.2 10 pH 4.2: about 40% of maximal activity, pH 10.0: about 55% of maximal activity Aspergillus sydowii

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 8.5 below 45°C, stable Aspergillus sydowii