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Literature summary for 3.2.1.147 extracted from

  • Henderson, H.M.; McEwen, T.J.
    Effect of ascorbic acid on thioglucosidases from different crucifers (1972), Phytochemistry, 11, 3127-3133.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
ascorbic acid
-
Brassica napus
ascorbic acid considerable activation in the pH range 5.7-7.5, maximal activation at 0.375 mM Sinapis alba

Organism

Organism UniProt Comment Textmining
Brassica napus
-
cv. Turret
-
Brassica napus
-
cv. Bronowski
-
Brassica rapa
-
cv. Echo
-
Sinapis alba
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sinigrin + H2O
-
Brassica napus D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
?
sinigrin + H2O
-
Brassica rapa D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
?
sinigrin + H2O
-
Sinapis alba D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
?

pH Range

pH Minimum pH Maximum Comment Organism
6 7.2 activity in presence of ascorbic acid is greater in sodium phosphate buffer than in citric acid /Na2HPO4 buffer Brassica napus