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Literature summary for 3.2.1.147 extracted from
- Studies on the myrosinase in mustard seed. Part III. On the effect of neutral salts (1968), Agric. Biol. Chem., 32, 479-483.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| KNO3 | activates in absence of ascorbate, inhibits in presence of 1 mM ascorbate | Brassica juncea |  |
| NaBr | activates in absence of ascorbate, inhibits in presence of 1 mM ascorbate | Brassica juncea | |
| NaNO3 | activates in absence of ascorbate, inhibits in presence of 1 mM ascorbate | Brassica juncea | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.094 | - |
sinigrin | in presence of 0.05 M NaCl | Brassica juncea | |
| 0.18 | - |
sinigrin | in presence of 0.5 M NaCl | Brassica juncea | |
| 0.26 | - |
sinigrin | in presence of 0.05 M NaCl and 1 mM ascorbate | Brassica juncea | |
| 0.64 | - |
sinigrin | in presence of 2.5 M NaCl | Brassica juncea | |
| 0.92 | - |
sinigrin | in presence of 0.5 M NaCl and 1 mM ascorbate | Brassica juncea | |
| 2.78 | - |
sinigrin | in presence of 2.5 M NaCl and 1 mM ascorbate | Brassica juncea | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| (NH4)2SO4 | activation is stronger in presence of 1 mM ascorbate | Brassica juncea | |
| Ba2+ | activation is stronger in presence of 1 mM ascorbate | Brassica juncea | |
| Ca2+ | slight activation in presence of 1 mM ascorbate, no effect in absence of ascorbate | Brassica juncea | |
| K+ | K2SO4, activation is stronger in presence of 1 mM ascorbate | Brassica juncea | |
| KNO3 | activates in absence of ascorbate, inhibits in presence of 1 mM ascorbate | Brassica juncea | |
| Mg2+ | activation is stronger in presence of 1 mM ascorbate | Brassica juncea | |
| Na+ | Na2SO4, activation is stronger in presence of 1 mM ascorbate | Brassica juncea | |
| NaBr | activates in absence of ascorbate, inhibits in presence of 1 mM ascorbate | Brassica juncea | |
| NaNO3 | activates in absence of ascorbate, inhibits in presence of 1 mM ascorbate | Brassica juncea | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brassica juncea | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | - |
Brassica juncea | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sinigrin + H2O | - |
Brassica juncea | D-glucose + 3-isothiocyanatoprop-1-ene + SO42- | - |
? | |
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