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Literature summary for 3.2.1.147 extracted from
- A steady-state kinetics study of myrosinase with direct ultraviolet spectrophotometric assay (1982), Anal. Biochem., 123, 320-324.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.156 | - |
sinigrin | at pH 7.0, at 30°C | Sinapis alba |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sinapis alba | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | - |
Sinapis alba | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
direct ultraviolet spectrophotometric assay | Sinapis alba |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | hydrolysis of mustard oil glucosides to isothiocyanate, glucose and sulfate | Sinapis alba | ? | - |
? | |
| sinigrin + H2O | - |
Sinapis alba | D-glucose + 3-isothiocyanatoprop-1-ene + SO42- | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 7.8 | hydrolysis of sinigrin, in citrate and phosphate buffer | Sinapis alba |
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Release 2023.1 (January 2023)
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