Literature summary for 3.2.1.147 extracted from

Petroski, R.J.; Kwolek, W.F.
Interaction of a fungal thioglucoside glycohydrolase and cruciferous plant epithiospecifier protein to form 1-cyanoepithioalkanes: implication of an allosteric mechanism (1985), Phytochemistry, 24, 213-216.

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Activating Compound

Activating Compound	Comment	Organism	Structure
epithiospecifier protein	ESP, is a small protein of molecular weight 30 to 40 kDa, which co-occurs with myrosinase. ESP does not have thioglucosidase activity, but interacts with myrosinase to promote the transfer of sulfur from the S-glucose moiety of terminally unsaturated glucosinolates to the alkenyl moiety, resulting in the formation of epithionitriles. The presence of ferrous ions are essential for ESP function.	Aspergillus sydowii
epithiospecifier protein	ESP, is a small protein of molecular weight 30 to 40 kDa, which co-occurs with myrosinase. ESP does not have thioglucosidase activity, but interacts with myrosinase to promote the transfer of sulfur from the S-glucose moiety of terminally unsaturated glucosinolates to the alkenyl moiety, resulting in the formation of epithionitriles. The presence of ferrous ions are essential for ESP function.	Sinapis alba
epithiospecifier protein	in presence of epithiospecifier protein 1-cyano-2,3-epithiopropane and allyl isothiocyanate are formed, in absence of epithiospecifier protein only allyl isothiocyanate is formed	Aspergillus sydowii
epithiospecifier protein	in presence of epithiospecifier protein 1-cyano-2,3-epithiopropane and allyl isothiocyanate are formed, in absence of epithiospecifier protein only allyl isothiocyanate is formed	Sinapis alba

Organism

Organism	UniProt	Comment	Textmining
Aspergillus sydowii	-	-	-
Sinapis alba	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
allylglucosinolate + H2O	-	Aspergillus sydowii	additional information	in presence of epithiospecifier protein 1-cyano-2,3-epithiopropane and allyl isothiocyanate are formed, in absence of epithiospecifier protein only allyl isothiocyanate is formed	?
allylglucosinolate + H2O	-	Sinapis alba	additional information	in presence of epithiospecifier protein 1-cyano-2,3-epithiopropane and allyl isothiocyanate are formed, in absence of epithiospecifier protein only allyl isothiocyanate is formed	?
sinigrin + H2O	-	Aspergillus sydowii	D-glucose + 3-isothiocyanatoprop-1-ene + SO42-	-	?
sinigrin + H2O	-	Sinapis alba	D-glucose + 3-isothiocyanatoprop-1-ene + SO42-	-	?

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Release 2023.1 (January 2023)