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Literature summary for 3.2.1.147 extracted from

  • Bones, A.; Slupphaug, G.
    Purification, characterization and partial amino acid sequencing of beta-thioglucosidase from Brassica napus (1989), J. Plant Physiol., 134, 722-729.
No PubMed abstract available
Search for more literature for 3.2.1.147

Activating Compound

Activating Compound Comment Organism Structure
ascorbic acid maximal activation at 0.3-0.5 mM Brassica napus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 sinigrin
-
 Brassica napus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
77000
-
 2 * 77000, SDS-PAGE Brassica napus
154000
-
 non-denaturing PAGE Brassica napus

Organism

Organism UniProt Comment Textmining
Brassica napus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein contains fucose, mannose and N-acetylglucosamine. Enzyme form Ca contains 18.9% carbohydrate, enzyme form Cb contains 11.7% carbohydrate, enzyme form Cc contains 9.6% carbohydrate Brassica napus

Purification (Commentary)

Purification (Comment) Organism
three different forms of enzyme: Ca, Cb and Cc Brassica napus

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
 Brassica napus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
25.33
-
 enzyme form Cc Brassica napus
28.94
-
 enzyme form Ca Brassica napus
32.11
-
 enzyme form Cb Brassica napus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sinigrin + H2O 2-propenylglucosinolate Brassica napus D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 77000, SDS-PAGE Brassica napus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Brassica napus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 80 40°C: about 55% of maximal activity of enzyme form Ca, about 50% of maximal activity of enzyme form Cb, about 60% of maximal activity of enzyme form Cc, 80°C: about 55% of maximal activity of enzyme form Ca, about 60% of maximal activity of enzyme form Cb, about 60% of maximal activity of enzyme form Cc Brassica napus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2 5.5
-
 Brassica napus

pH Range

pH Minimum pH Maximum Comment Organism
4 7 about 35% of maximal activity of enzyme form Cc and Cb, about 45% of maximal activity of enzyme form Ca at pH 4.0 and at pH 7.0 Brassica napus