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Literature summary for 3.2.1.146 extracted from

  • Matsunaga, E.; Higuchi, Y.; Mori, K.; Yairo, N.; Toyota, S.; Oka, T.; Tashiro, K.; Takegawa, K.
    Characterization of a PA14 domain-containing galactofuranose-specific beta-D-galactofuranosidase from Streptomyces sp. (2017), Biosci. Biotechnol. Biochem., 81, 1314-1319 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
orf0643, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of N-terminally 2 x His6- and Nus-tagged enzyme in Escherichia coli strain BL21(DE3)CodonPlus Streptomyces sp. JHA26

Protein Variants

Protein Variants Comment Organism
additional information truncation of the PA14 domain of the enzyme (N-terminal 179 amino acid residues), ORF0643 Galf-ase lacking the PA14 domain exhibits about half activity compared with full-length ORF0643 Streptomyces sp. JHA26

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.8
-
4-nitrophenyl beta-D-galactofuranoside pH 4.5, 37°C, recombinant enzyme Streptomyces sp. JHA26

Organism

Organism UniProt Comment Textmining
Streptomyces sp. JHA26 A0A1Q2U3E4 isolated from soil in Kagawa University, Japan
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally 2 x His6- and Nus-tagged enzyme from Escherichia coli strain BL21(DE3)CodonPlus, by nickel affinity chromatography, tag cleavage by HRV3C protease, followed by nickel affinity chromatography and ultrafiltration Streptomyces sp. JHA26

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-galactofuranoside + H2O
-
Streptomyces sp. JHA26 4-nitrophenol + D-galactofuranose
-
?
additional information the enzyme is active with a galactofuranose-containing oligosaccharide termed GM Streptomyces sp. JHA26 ?
-
?

Synonyms

Synonyms Comment Organism
beta-D-galactofuranosidase
-
Streptomyces sp. JHA26
galactofuranose-specific beta-D-galactofuranosidase
-
Streptomyces sp. JHA26
Galf-ase
-
Streptomyces sp. JHA26
Galf-specific Galf-ase
-
Streptomyces sp. JHA26
Galf-specific hydrolase
-
Streptomyces sp. JHA26
ORF0643 locus name Streptomyces sp. JHA26

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Streptomyces sp. JHA26

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
purified recombinant detagged enzyme, pH 5.5, 10 min, stable up to, unstable above Streptomyces sp. JHA26

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
recombinant enzyme Streptomyces sp. JHA26

General Information

General Information Comment Organism
additional information ORF0643 contains PA14, glycosyl hydro 2 N, glycosyl hydro and glycosyl hydro 2C domains Streptomyces sp. JHA26