BRENDA - Enzyme Database
show all sequences of 3.2.1.145

Degradative enzymes for type II arabinogalactan side chains in Bifidobacterium longum subsp. longum

Fujita, K.; Sakamoto, A.; Kaneko, S.; Kotake, T.; Tsumuraya, Y.; Kitahara, K.; Appl. Microbiol. Biotechnol. 103, 1299-1310 (2019)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
DNA and amino acid sequence determination and analysis, domain structure, phylogenetic analysis, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
Bifidobacterium longum subsp. Longum
Organism
Organism
UniProt
Commentary
Textmining
Bifidobacterium longum subsp. Longum
A0A401ETL2
-
-
Purification (Commentary)
Purification (Commentary)
Organism
recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3)
Bifidobacterium longum subsp. Longum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
beta-1,6-Gal2-galactitol + H2O
the enzyme specifically releases beta-1,6-Gal2 but not galactose from beta-1,6-Gal2-Gat
752571
Bifidobacterium longum subsp. Longum
beta-1,6-Gal2 + galactitol
-
-
-
?
beta-1,6-Gal3 + H2O
-
752571
Bifidobacterium longum subsp. Longum
beta-1,6-Gal2 + D-galactose
-
-
-
?
beta-1,6-galactooligosaccharides + H2O
the enzyme releases beta-1,6-Gal2 from beta-1,6-galactooligosaccharides (beta-1,6-Gal>9) in a time-dependent manner with low rates of beta-1,6-Gal3 and beta-1,6-Gal4
752571
Bifidobacterium longum subsp. Longum
beta-1,6-Gal2 + ?
-
-
-
?
larch type II arabinogalactan + H2O
degradation profiles of type II AG
752571
Bifidobacterium longum subsp. Longum
?
-
-
-
?
larch type II arabinogalactan + H2O
release of galactobiose, beta-1,6-Gal2, degradation profiles of type II AG
752571
Bifidobacterium longum subsp. Longum
beta-1,6-Gal2 + ?
-
-
-
?
additional information
the recombinant exo-beta-1,6-galactobiohydrolase specifically releases beta-1,6-galactobiose (beta-1,6-Gal2) from the nonreducing terminal of beta-1,6-galactooligosaccharides. beta-1,6-Gal2 is additively released from larch type II arabinogalactan (AG) by the combined use of type II AG degradative enzymes, including Bl1,3Gal, Bl1,6Gal, and BlArafA. GH30_5 exo-beta-1,6-galactobiohydrolase (Bl1,6Gal, EC 3.2.1.145) and GH43_22 alpha-L-arabinofuranosidase (BlArafA, EC 3.2.1.55) are degradative enzymes for type II arabinogalactan (AG) side chains in cooperation with GH43_24 exo-beta-1,3-galactanase (Bl1,3Gal, EC 3.2.1.145). Degradative mechanism, overview
752571
Bifidobacterium longum subsp. Longum
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 116000, recombinant His-tagged enzyme, SDS-PAGE, x * 113302, His-tagged enzyme, sequence calculation
Bifidobacterium longum subsp. Longum
Synonyms
Synonyms
Commentary
Organism
Bl1,3Gal
-
Bifidobacterium longum subsp. Longum
Bl1,6Gal
-
Bifidobacterium longum subsp. Longum
exo-beta-1,3-galactanase
-
Bifidobacterium longum subsp. Longum
exo-beta-1,6-galactobiohydrolase
-
Bifidobacterium longum subsp. Longum
GH30_5 exo-beta-1,6-galactobiohydrolase
-
Bifidobacterium longum subsp. Longum
GH43_24 exo-beta-1,3-galactanase
-
Bifidobacterium longum subsp. Longum
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
assay at
Bifidobacterium longum subsp. Longum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.5
-
assay at
Bifidobacterium longum subsp. Longum
Cloned(Commentary) (protein specific)
Commentary
Organism
DNA and amino acid sequence determination and analysis, domain structure, phylogenetic analysis, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
Bifidobacterium longum subsp. Longum
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3)
Bifidobacterium longum subsp. Longum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
beta-1,6-Gal2-galactitol + H2O
the enzyme specifically releases beta-1,6-Gal2 but not galactose from beta-1,6-Gal2-Gat
752571
Bifidobacterium longum subsp. Longum
beta-1,6-Gal2 + galactitol
-
-
-
?
beta-1,6-Gal3 + H2O
-
752571
Bifidobacterium longum subsp. Longum
beta-1,6-Gal2 + D-galactose
-
-
-
?
beta-1,6-galactooligosaccharides + H2O
the enzyme releases beta-1,6-Gal2 from beta-1,6-galactooligosaccharides (beta-1,6-Gal>9) in a time-dependent manner with low rates of beta-1,6-Gal3 and beta-1,6-Gal4
752571
Bifidobacterium longum subsp. Longum
beta-1,6-Gal2 + ?
-
-
-
?
larch type II arabinogalactan + H2O
degradation profiles of type II AG
752571
Bifidobacterium longum subsp. Longum
?
-
-
-
?
larch type II arabinogalactan + H2O
release of galactobiose, beta-1,6-Gal2, degradation profiles of type II AG
752571
Bifidobacterium longum subsp. Longum
beta-1,6-Gal2 + ?
-
-
-
?
additional information
the recombinant exo-beta-1,6-galactobiohydrolase specifically releases beta-1,6-galactobiose (beta-1,6-Gal2) from the nonreducing terminal of beta-1,6-galactooligosaccharides. beta-1,6-Gal2 is additively released from larch type II arabinogalactan (AG) by the combined use of type II AG degradative enzymes, including Bl1,3Gal, Bl1,6Gal, and BlArafA. GH30_5 exo-beta-1,6-galactobiohydrolase (Bl1,6Gal, EC 3.2.1.145) and GH43_22 alpha-L-arabinofuranosidase (BlArafA, EC 3.2.1.55) are degradative enzymes for type II arabinogalactan (AG) side chains in cooperation with GH43_24 exo-beta-1,3-galactanase (Bl1,3Gal, EC 3.2.1.145). Degradative mechanism, overview
752571
Bifidobacterium longum subsp. Longum
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 116000, recombinant His-tagged enzyme, SDS-PAGE, x * 113302, His-tagged enzyme, sequence calculation
Bifidobacterium longum subsp. Longum
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
assay at
Bifidobacterium longum subsp. Longum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.5
-
assay at
Bifidobacterium longum subsp. Longum
General Information
General Information
Commentary
Organism
evolution
the enzyme belongs to the glycosyl hydrolase family 30, GH30, subfamily 22. GH43_24 exo-beta-1,3-galactanase (Bl1,3Gal, EC 3.2.1.145) forms a gene cluster with GH30_5 and GH43_22 enzyme candidates, which are conserved in all Bifidobacterium longum strains; the enzyme belongs to the glycosyl hydrolase family 43, GH43, subfamily 24. GH43_24 exo-beta-1,3-galactanase (Bl1,3Gal) forms a gene cluster with GH30_5 exo-beta-1,6-galactobiohydrolase (Bl1,6Gal) and GH43_22 alpha-L-arabinofuranosidase (BlArafA) enzyme candidates, which are conserved in all Bifidobacterium longum strains
Bifidobacterium longum subsp. Longum
metabolism
GH30_5 exo-beta-1,6-galactobiohydrolase (Bl1,6Gal, EC 3.2.1.145) and GH43_22 alpha-L-arabinofuranosidase (BlArafA, EC 3.2.1.55) are degradative enzymes for type II arabinogalactan (AG) side chains in cooperation with GH43_24 exo-beta-1,3-galactanase (Bl1,3Gal, EC 3.2.1.145); GH30_5 exo-beta-1,6-galactobiohydrolase (Bl1,6Gal, EC 3.2.1.145) and GH43_22 alpha-L-arabinofuranosidase (BlArafA, EC 3.2.1.55), which are degradative enzymes for type II arabinogalactan (AG) side chains in cooperation with GH43_24 exo-beta-1,3-galactanase (Bl1,3Gal, EC 3.2.1.145)
Bifidobacterium longum subsp. Longum
physiological function
type II arabinogalactan (AG) degradative enzymes, including Bl1,3Gal, Bl1,6Gal, and BlArafA. GH30_5 exo-beta-1,6-galactobiohydrolase (Bl1,6Gal, EC 3.2.1.145) and GH43_22 alpha-L-arabinofuranosidase (BlArafA, EC 3.2.1.55) are important for the acquisition of type II AG in Bifidobacterium longum
Bifidobacterium longum subsp. Longum
General Information (protein specific)
General Information
Commentary
Organism
evolution
the enzyme belongs to the glycosyl hydrolase family 30, GH30, subfamily 22. GH43_24 exo-beta-1,3-galactanase (Bl1,3Gal, EC 3.2.1.145) forms a gene cluster with GH30_5 and GH43_22 enzyme candidates, which are conserved in all Bifidobacterium longum strains; the enzyme belongs to the glycosyl hydrolase family 43, GH43, subfamily 24. GH43_24 exo-beta-1,3-galactanase (Bl1,3Gal) forms a gene cluster with GH30_5 exo-beta-1,6-galactobiohydrolase (Bl1,6Gal) and GH43_22 alpha-L-arabinofuranosidase (BlArafA) enzyme candidates, which are conserved in all Bifidobacterium longum strains
Bifidobacterium longum subsp. Longum
metabolism
GH30_5 exo-beta-1,6-galactobiohydrolase (Bl1,6Gal, EC 3.2.1.145) and GH43_22 alpha-L-arabinofuranosidase (BlArafA, EC 3.2.1.55) are degradative enzymes for type II arabinogalactan (AG) side chains in cooperation with GH43_24 exo-beta-1,3-galactanase (Bl1,3Gal, EC 3.2.1.145); GH30_5 exo-beta-1,6-galactobiohydrolase (Bl1,6Gal, EC 3.2.1.145) and GH43_22 alpha-L-arabinofuranosidase (BlArafA, EC 3.2.1.55), which are degradative enzymes for type II arabinogalactan (AG) side chains in cooperation with GH43_24 exo-beta-1,3-galactanase (Bl1,3Gal, EC 3.2.1.145)
Bifidobacterium longum subsp. Longum
physiological function
type II arabinogalactan (AG) degradative enzymes, including Bl1,3Gal, Bl1,6Gal, and BlArafA. GH30_5 exo-beta-1,6-galactobiohydrolase (Bl1,6Gal, EC 3.2.1.145) and GH43_22 alpha-L-arabinofuranosidase (BlArafA, EC 3.2.1.55) are important for the acquisition of type II AG in Bifidobacterium longum
Bifidobacterium longum subsp. Longum
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
752571
Fujita
Degradative enzymes for type ...
Bifidobacterium longum subsp. Longum
Appl. Microbiol. Biotechnol.
103
1299-1310
2019
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-
1
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3
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1
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6
1
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1
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1
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1
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6
1
1
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1
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3
3
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-
752397
Godoy
Crystal structure of a putati ...
Bifidobacterium bifidum, Bifidobacterium bifidum NCIMB 41171, Bifidobacterium bifidum S17
Acta Crystallogr. Sect. F
72
288-293
2016
-
-
1
1
-
-
-
-
-
-
1
-
-
5
-
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1
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-
-
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3
2
2
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1
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1
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1
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1
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3
2
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-
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1
1
-
-
-
731184
Fujita
Bifidobacterium longum subsp. ...
Bifidobacterium longum, Bifidobacterium longum JCM 1217
Appl. Environ. Microbiol.
80
4577-4584
2014
-
-
1
-
1
-
-
1
-
-
1
-
-
6
-
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9
-
14
-
2
1
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1
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1
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1
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1
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1
-
-
-
-
-
-
-
9
-
14
-
1
-
-
-
1
-
-
-
1
2
2
1
-
-
731202
Okawa
Identification of an exo-beta- ...
Fusarium oxysporum 12S, Fusarium oxysporum
Appl. Microbiol. Biotechnol.
97
9685-9694
2013
-
-
1
-
-
-
-
-
-
-
-
-
-
5
-
-
1
-
-
-
1
-
12
1
2
1
-
-
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1
-
-
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-
-
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-
-
1
-
-
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1
-
-
1
-
12
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-
731581
Ling
An exo-beta-(1->3)-D-galactana ...
Streptomyces sp. 19(2012), Streptomyces sp.
Carbohydr. Res.
352
70-81
2012
-
1
1
-
-
-
-
-
-
-
3
4
-
6
-
-
1
-
-
-
1
-
12
1
4
1
-
-
-
2
-
-
-
-
1
-
-
2
2
-
-
-
-
-
-
-
-
-
-
3
4
-
-
-
2
-
-
2
-
12
2
2
-
-
-
2
-
-
2
-
1
2
-
-
-
732396
Jiang
Crystal structure of 1,3Gal43A ...
Hungateiclostridium thermocellum
J. Struct. Biol.
180
447-457
2012
-
-
1
1
2
-
-
-
-
-
2
-
-
4
-
-
1
-
-
-
-
-
1
2
3
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
2
-
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-
-
-
-
-
2
-
-
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-
1
-
-
-
-
1
2
1
-
-
-
1
-
-
-
-
2
2
-
-
-
713904
Sakamoto
Characterization of an exo-bet ...
Sphingomonas sp., Sphingomonas sp. 24T
Appl. Microbiol. Biotechnol.
90
1701-1710
2011
1
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
1
-
-
1
2
-
12
1
2
1
-
1
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1
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1
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1
-
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-
-
-
-
-
-
-
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-
1
-
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1
-
1
2
-
12
1
1
-
1
-
1
-
1
-
-
-
-
-
-
-
703061
Ling
Preparation of a new chromogen ...
Irpex lacteus
Carbohydr. Res.
344
1941-1946
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
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1
1
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1
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2
1
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1
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-
-
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-
-
1
1
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
706965
Ishida
Crystallization of selenomethi ...
Phanerochaete chrysosporium
Acta Crystallogr. Sect. F
65
1274-1276
2009
-
-
1
1
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
1
3
-
-
-
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-
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1
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1
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1
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-
-
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
707784
Kotake
Molecular cloning and expressi ...
Irpex lacteus, Irpex lacteus NBRC 5367
Biosci. Biotechnol. Biochem.
73
2303-2309
2009
-
-
1
-
-
-
-
-
-
-
1
-
-
2
-
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1
-
-
-
1
-
4
1
2
1
1
-
-
1
1
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-
-
-
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-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
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1
-
-
1
-
4
1
1
1
-
-
1
1
-
-
-
-
-
-
-
-
677662
Ichinose
Characterization of an exo-bet ...
Hungateiclostridium thermocellum
Appl. Environ. Microbiol.
72
3515-3523
2006
-
-
1
-
-
-
-
-
-
-
1
-
-
4
-
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1
-
-
-
1
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5
-
2
2
-
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1
1
-
-
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-
-
1
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-
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-
-
-
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1
-
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1
-
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1
-
5
-
2
-
-
-
1
1
-
-
-
-
-
-
-
-
678786
Ichinose
Characterization of an exo-bet ...
Streptomyces avermitilis, Streptomyces avermitilis NBRC 14893
Biosci. Biotechnol. Biochem.
70
2745-2750
2006
-
-
1
-
-
-
-
-
-
-
1
-
-
4
-
1
1
-
-
-
1
-
10
-
2
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
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-
1
1
-
-
1
-
10
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
666634
Kotake
Molecular cloning of a b-galac ...
Raphanus sativus
Plant Physiol.
138
1563-1576
2005
-
-
1
-
-
-
4
3
-
-
2
-
-
4
-
-
1
-
-
-
1
-
10
1
1
1
-
1
3
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
4
-
3
-
-
2
-
-
-
-
1
-
-
1
-
10
1
1
-
1
3
1
1
-
-
-
-
-
-
-
-
171335
Tsumuraya
Purification of an exo-beta-(1 ...
Irpex lacteus
J. Biol. Chem.
265
7207-7215
1990
-
-
-
-
-
-
-
2
-
-
2
1
-
1
-
-
1
-
-
-
1
-
4
1
-
-
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
2
1
-
-
-
1
-
-
1
-
4
1
-
1
1
-
1
-
1
-
-
-
-
-
-
-