BRENDA - Enzyme Database
show all sequences of 3.2.1.145

Identification of an exo-beta-1,3-D-galactanase from Fusarium oxysporum and the synergistic effect with related enzymes on degradation of type II arabinogalactan

Okawa, M.; Fukamachi, K.; Tanaka, H.; Sakamoto, T.; Appl. Microbiol. Biotechnol. 97, 9685-9694 (2013)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene Fo1,3 gal, recombinant expression in Pichia pastoris strain KM71H, subcloning in Escherichia coli strain DH5alpha. The expression vector pPICZ?A contains the native Saccharomyces cerevisiae alpha-factor secretion signal, which allows Pichia pastoris to secrete heterologous proteins into the growth medium
Fusarium oxysporum
Organism
Organism
UniProt
Commentary
Textmining
Fusarium oxysporum
B6F260
gene Fo1,3 gal
-
Fusarium oxysporum 12S
B6F260
gene Fo1,3 gal
-
Purification (Commentary)
Purification (Commentary)
Organism
recombinant soluble extracellular enzyme 5.7fold from Pichia pastoris strain KM71H by anion exchange and hydrophobic interaction chromatography
Fusarium oxysporum
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
18.9
-
purified recombinant enzyme, pH 5.0, 30°C, beta-1,3-galactan as substrate
Fusarium oxysporum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
beta-1,3-D-galactobiose + H2O
-
731202
Fusarium oxysporum
D-galactose
-
-
-
?
beta-1,3-D-galactobiose + H2O
-
731202
Fusarium oxysporum 12S
D-galactose
-
-
-
?
beta-1,3-galactan + H2O
preferred substrate
731202
Fusarium oxysporum
D-galactose
-
-
-
?
beta-1,3-galactan + H2O
preferred substrate
731202
Fusarium oxysporum 12S
D-galactose
-
-
-
?
gum arabic + H2O
-
731202
Fusarium oxysporum
?
-
-
-
?
gum arabic + H2O
-
731202
Fusarium oxysporum 12S
?
-
-
-
?
larch wood arabinogalactan + H2O
the enzyme releases galactose, beta-1,6-D-galactobiose, beta-1,6-D-galactotriose, and beta-1,6-D-galactotriose substituted with a single arabinofuranose residue accompanied with unidentified oligosaccharides, indicating that the enzyme can by-pass the branching points of beta-1,3-galactan backbones
731202
Fusarium oxysporum
?
-
-
-
?
larch wood arabinogalactan + H2O
the enzyme releases galactose, beta-1,6-D-galactobiose, beta-1,6-D-galactotriose, and beta-1,6-D-galactotriose substituted with a single arabinofuranose residue accompanied with unidentified oligosaccharides, indicating that the enzyme can by-pass the branching points of beta-1,3-galactan backbones
731202
Fusarium oxysporum 12S
?
-
-
-
?
additional information
no activity of the recombinant enzyme with 4-nitrophenyl beta-D-galactopyranoside, beta-1,4-D-galactobiose, or beta-1,6-D-galactobiose, and no activity with birchwood xylan, lupin galactan, carob galactomannan, sugar beet L-arabinan, and wheat arabinoxylan
731202
Fusarium oxysporum
?
-
-
-
-
additional information
no activity of the recombinant enzyme with 4-nitrophenyl beta-D-galactopyranoside, beta-1,4-D-galactobiose, or beta-1,6-D-galactobiose, and no activity with birchwood xylan, lupin galactan, carob galactomannan, sugar beet L-arabinan, and wheat arabinoxylan
731202
Fusarium oxysporum 12S
?
-
-
-
-
sugar beet debranched L-arabinan + H2O
-
731202
Fusarium oxysporum
?
-
-
-
?
type II arabinogalactan + H2O
-
731202
Fusarium oxysporum
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
More
the enzyme contains a GH43_6 domain and a CBM35_galactosidase-like domain
Fusarium oxysporum
Synonyms
Synonyms
Commentary
Organism
exo-beta-1,3-D-galactanase
-
Fusarium oxysporum
Fo/1,3Gal
-
Fusarium oxysporum
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Fusarium oxysporum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
-
-
Fusarium oxysporum
Cloned(Commentary) (protein specific)
Commentary
Organism
gene Fo1,3 gal, recombinant expression in Pichia pastoris strain KM71H, subcloning in Escherichia coli strain DH5alpha. The expression vector pPICZ?A contains the native Saccharomyces cerevisiae alpha-factor secretion signal, which allows Pichia pastoris to secrete heterologous proteins into the growth medium
Fusarium oxysporum
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant soluble extracellular enzyme 5.7fold from Pichia pastoris strain KM71H by anion exchange and hydrophobic interaction chromatography
Fusarium oxysporum
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
18.9
-
purified recombinant enzyme, pH 5.0, 30°C, beta-1,3-galactan as substrate
Fusarium oxysporum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
beta-1,3-D-galactobiose + H2O
-
731202
Fusarium oxysporum
D-galactose
-
-
-
?
beta-1,3-D-galactobiose + H2O
-
731202
Fusarium oxysporum 12S
D-galactose
-
-
-
?
beta-1,3-galactan + H2O
preferred substrate
731202
Fusarium oxysporum
D-galactose
-
-
-
?
beta-1,3-galactan + H2O
preferred substrate
731202
Fusarium oxysporum 12S
D-galactose
-
-
-
?
gum arabic + H2O
-
731202
Fusarium oxysporum
?
-
-
-
?
gum arabic + H2O
-
731202
Fusarium oxysporum 12S
?
-
-
-
?
larch wood arabinogalactan + H2O
the enzyme releases galactose, beta-1,6-D-galactobiose, beta-1,6-D-galactotriose, and beta-1,6-D-galactotriose substituted with a single arabinofuranose residue accompanied with unidentified oligosaccharides, indicating that the enzyme can by-pass the branching points of beta-1,3-galactan backbones
731202
Fusarium oxysporum
?
-
-
-
?
larch wood arabinogalactan + H2O
the enzyme releases galactose, beta-1,6-D-galactobiose, beta-1,6-D-galactotriose, and beta-1,6-D-galactotriose substituted with a single arabinofuranose residue accompanied with unidentified oligosaccharides, indicating that the enzyme can by-pass the branching points of beta-1,3-galactan backbones
731202
Fusarium oxysporum 12S
?
-
-
-
?
additional information
no activity of the recombinant enzyme with 4-nitrophenyl beta-D-galactopyranoside, beta-1,4-D-galactobiose, or beta-1,6-D-galactobiose, and no activity with birchwood xylan, lupin galactan, carob galactomannan, sugar beet L-arabinan, and wheat arabinoxylan
731202
Fusarium oxysporum
?
-
-
-
-
additional information
no activity of the recombinant enzyme with 4-nitrophenyl beta-D-galactopyranoside, beta-1,4-D-galactobiose, or beta-1,6-D-galactobiose, and no activity with birchwood xylan, lupin galactan, carob galactomannan, sugar beet L-arabinan, and wheat arabinoxylan
731202
Fusarium oxysporum 12S
?
-
-
-
-
sugar beet debranched L-arabinan + H2O
-
731202
Fusarium oxysporum
?
-
-
-
?
type II arabinogalactan + H2O
-
731202
Fusarium oxysporum
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
More
the enzyme contains a GH43_6 domain and a CBM35_galactosidase-like domain
Fusarium oxysporum
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Fusarium oxysporum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
-
-
Fusarium oxysporum
General Information
General Information
Commentary
Organism
metabolism
the enzyme shows a synergistic effect with related enzymes on degradation of type II arabinogalactan
Fusarium oxysporum
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme shows a synergistic effect with related enzymes on degradation of type II arabinogalactan
Fusarium oxysporum
Other publictions for EC 3.2.1.145
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
752571
Fujita
Degradative enzymes for type ...
Bifidobacterium longum subsp. Longum
Appl. Microbiol. Biotechnol.
103
1299-1310
2019
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
6
1
6
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
6
1
1
-
-
-
1
-
-
-
-
3
3
-
-
-
752397
Godoy
Crystal structure of a putati ...
Bifidobacterium bifidum, Bifidobacterium bifidum NCIMB 41171, Bifidobacterium bifidum S17
Acta Crystallogr. Sect. F
72
288-293
2016
-
-
1
1
-
-
-
-
-
-
1
-
-
5
-
-
1
-
-
-
-
-
3
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
3
2
-
-
-
-
-
-
-
-
-
1
1
-
-
-
731184
Fujita
Bifidobacterium longum subsp. ...
Bifidobacterium longum, Bifidobacterium longum JCM 1217
Appl. Environ. Microbiol.
80
4577-4584
2014
-
-
1
-
1
-
-
1
-
-
1
-
-
6
-
-
-
-
-
-
9
-
14
-
2
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
9
-
14
-
1
-
-
-
1
-
-
-
1
2
2
1
-
-
731202
Okawa
Identification of an exo-beta- ...
Fusarium oxysporum 12S, Fusarium oxysporum
Appl. Microbiol. Biotechnol.
97
9685-9694
2013
-
-
1
-
-
-
-
-
-
-
-
-
-
5
-
-
1
-
-
-
1
-
12
1
2
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
12
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-
731581
Ling
An exo-beta-(1->3)-D-galactana ...
Streptomyces sp. 19(2012), Streptomyces sp.
Carbohydr. Res.
352
70-81
2012
-
1
1
-
-
-
-
-
-
-
3
4
-
6
-
-
1
-
-
-
1
-
12
1
4
1
-
-
-
2
-
-
-
-
1
-
-
2
2
-
-
-
-
-
-
-
-
-
-
3
4
-
-
-
2
-
-
2
-
12
2
2
-
-
-
2
-
-
2
-
1
2
-
-
-
732396
Jiang
Crystal structure of 1,3Gal43A ...
Hungateiclostridium thermocellum
J. Struct. Biol.
180
447-457
2012
-
-
1
1
2
-
-
-
-
-
2
-
-
4
-
-
1
-
-
-
-
-
1
2
3
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
2
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
1
2
1
-
-
-
1
-
-
-
-
2
2
-
-
-
713904
Sakamoto
Characterization of an exo-bet ...
Sphingomonas sp., Sphingomonas sp. 24T
Appl. Microbiol. Biotechnol.
90
1701-1710
2011
1
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
1
-
-
1
2
-
12
1
2
1
-
1
-
1
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
2
-
12
1
1
-
1
-
1
-
1
-
-
-
-
-
-
-
703061
Ling
Preparation of a new chromogen ...
Irpex lacteus
Carbohydr. Res.
344
1941-1946
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
706965
Ishida
Crystallization of selenomethi ...
Phanerochaete chrysosporium
Acta Crystallogr. Sect. F
65
1274-1276
2009
-
-
1
1
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
1
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
707784
Kotake
Molecular cloning and expressi ...
Irpex lacteus, Irpex lacteus NBRC 5367
Biosci. Biotechnol. Biochem.
73
2303-2309
2009
-
-
1
-
-
-
-
-
-
-
1
-
-
2
-
-
1
-
-
-
1
-
4
1
2
1
1
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
4
1
1
1
-
-
1
1
-
-
-
-
-
-
-
-
677662
Ichinose
Characterization of an exo-bet ...
Hungateiclostridium thermocellum
Appl. Environ. Microbiol.
72
3515-3523
2006
-
-
1
-
-
-
-
-
-
-
1
-
-
4
-
-
1
-
-
-
1
-
5
-
2
2
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
5
-
2
-
-
-
1
1
-
-
-
-
-
-
-
-
678786
Ichinose
Characterization of an exo-bet ...
Streptomyces avermitilis, Streptomyces avermitilis NBRC 14893
Biosci. Biotechnol. Biochem.
70
2745-2750
2006
-
-
1
-
-
-
-
-
-
-
1
-
-
4
-
1
1
-
-
-
1
-
10
-
2
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
1
-
-
1
-
10
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
666634
Kotake
Molecular cloning of a b-galac ...
Raphanus sativus
Plant Physiol.
138
1563-1576
2005
-
-
1
-
-
-
4
3
-
-
2
-
-
4
-
-
1
-
-
-
1
-
10
1
1
1
-
1
3
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
4
-
3
-
-
2
-
-
-
-
1
-
-
1
-
10
1
1
-
1
3
1
1
-
-
-
-
-
-
-
-
171335
Tsumuraya
Purification of an exo-beta-(1 ...
Irpex lacteus
J. Biol. Chem.
265
7207-7215
1990
-
-
-
-
-
-
-
2
-
-
2
1
-
1
-
-
1
-
-
-
1
-
4
1
-
-
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
2
1
-
-
-
1
-
-
1
-
4
1
-
1
1
-
1
-
1
-
-
-
-
-
-
-