Cloned (Comment) | Organism |
---|---|
gene Fo1,3 gal, recombinant expression in Pichia pastoris strain KM71H, subcloning in Escherichia coli strain DH5alpha. The expression vector pPICZ?A contains the native Saccharomyces cerevisiae alpha-factor secretion signal, which allows Pichia pastoris to secrete heterologous proteins into the growth medium | Fusarium oxysporum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fusarium oxysporum | B6F260 | gene Fo1,3 gal | - |
Fusarium oxysporum 12S | B6F260 | gene Fo1,3 gal | - |
Purification (Comment) | Organism |
---|---|
recombinant soluble extracellular enzyme 5.7fold from Pichia pastoris strain KM71H by anion exchange and hydrophobic interaction chromatography | Fusarium oxysporum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
18.9 | - |
purified recombinant enzyme, pH 5.0, 30°C, beta-1,3-galactan as substrate | Fusarium oxysporum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-1,3-D-galactobiose + H2O | - |
Fusarium oxysporum | D-galactose | - |
? | |
beta-1,3-D-galactobiose + H2O | - |
Fusarium oxysporum 12S | D-galactose | - |
? | |
beta-1,3-galactan + H2O | preferred substrate | Fusarium oxysporum | oligo-beta-(1->3)-D-galactose + D-galactose | - |
? | |
beta-1,3-galactan + H2O | preferred substrate | Fusarium oxysporum 12S | oligo-beta-(1->3)-D-galactose + D-galactose | - |
? | |
gum arabic + H2O | - |
Fusarium oxysporum | ? | - |
? | |
gum arabic + H2O | - |
Fusarium oxysporum 12S | ? | - |
? | |
larch wood arabinogalactan + H2O | the enzyme releases galactose, beta-1,6-D-galactobiose, beta-1,6-D-galactotriose, and beta-1,6-D-galactotriose substituted with a single arabinofuranose residue accompanied with unidentified oligosaccharides, indicating that the enzyme can by-pass the branching points of beta-1,3-galactan backbones | Fusarium oxysporum | ? | - |
? | |
larch wood arabinogalactan + H2O | the enzyme releases galactose, beta-1,6-D-galactobiose, beta-1,6-D-galactotriose, and beta-1,6-D-galactotriose substituted with a single arabinofuranose residue accompanied with unidentified oligosaccharides, indicating that the enzyme can by-pass the branching points of beta-1,3-galactan backbones | Fusarium oxysporum 12S | ? | - |
? | |
additional information | no activity of the recombinant enzyme with 4-nitrophenyl beta-D-galactopyranoside, beta-1,4-D-galactobiose, or beta-1,6-D-galactobiose, and no activity with birchwood xylan, lupin galactan, carob galactomannan, sugar beet L-arabinan, and wheat arabinoxylan | Fusarium oxysporum | ? | - |
? | |
additional information | no activity of the recombinant enzyme with 4-nitrophenyl beta-D-galactopyranoside, beta-1,4-D-galactobiose, or beta-1,6-D-galactobiose, and no activity with birchwood xylan, lupin galactan, carob galactomannan, sugar beet L-arabinan, and wheat arabinoxylan | Fusarium oxysporum 12S | ? | - |
? | |
sugar beet debranched L-arabinan + H2O | - |
Fusarium oxysporum | ? | - |
? | |
type II arabinogalactan + H2O | - |
Fusarium oxysporum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme contains a GH43_6 domain and a CBM35_galactosidase-like domain | Fusarium oxysporum |
Synonyms | Comment | Organism |
---|---|---|
exo-beta-1,3-D-galactanase | - |
Fusarium oxysporum |
Fo/1,3Gal | - |
Fusarium oxysporum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Fusarium oxysporum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Fusarium oxysporum |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme shows a synergistic effect with related enzymes on degradation of type II arabinogalactan | Fusarium oxysporum |