BRENDA - Enzyme Database
show all sequences of 3.2.1.145

Bifidobacterium longum subsp. longum exo-beta-1,3-galactanase, an enzyme for the degradation of type II arabinogalactan

Fujita, K.; Sakaguchi, T.; Sakamoto, A.; Shimokawa, M.; Kitahara, K.; Appl. Environ. Microbiol. 80, 4577-4584 (2014)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
BLLJ_1840, phylogenetic tree, expresion as soluble enzyme in Escherichia coli
Bifidobacterium longum
Engineering
Protein Variants
Commentary
Organism
additional information
construction of deletion mutants NDELTA42, NDELTA42CDELTA257, NDELTA42CDELTA457, and NDELTA42CDELTA76 showing 100%, 80.1%, 122%, and 8.45% activity relative to wild-type enzyme activity
Bifidobacterium longum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic parameters of enzyme BLLJ_1840 activity on different substrates, overview
Bifidobacterium longum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
106100
-
sequence calculation
Bifidobacterium longum
Organism
Organism
UniProt
Commentary
Textmining
Bifidobacterium longum
A0A061M1M5
subsp. longum
-
Bifidobacterium longum JCM 1217
A0A061M1M5
subsp. longum
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.0139
-
substrate beta-1,3-D-galactotetraose, pH 5.5, 40C
Bifidobacterium longum
0.0157
-
substrate beta-1,3-D-galactopentaose, pH 5.5, 40C
Bifidobacterium longum
0.0188
-
substrate beta-1,3-D-galactotriose, pH 5.5, 40C
Bifidobacterium longum
0.0218
-
substrate beta-1,3-D-galactobiose, pH 5.5, 40C
Bifidobacterium longum
0.113
-
substrate gum arabic, pH 5.5, 40C
Bifidobacterium longum
1.01
-
substrate beta-1,3-galactan, pH 5.5, 40C
Bifidobacterium longum
1.17
-
substrate larch wood arabinogalactan, pH 5.5, 40C
Bifidobacterium longum
1.28
-
substrate dearabinosylated gum arabic, pH 5.5, 40C
Bifidobacterium longum
16.2
-
substrate dearabinosylated larch wood arabinogalactan, pH 5.5, 40C
Bifidobacterium longum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
beta-1,3-D-galactobiose + H2O
best beta-1,3-galactan substrate
731184
Bifidobacterium longum
2 D-galactose
-
-
-
?
beta-1,3-D-galactobiose + H2O
best beta-1,3-galactan substrate
731184
Bifidobacterium longum JCM 1217
2 D-galactose
-
-
-
?
beta-1,3-D-galactopentaose + H2O
-
731184
Bifidobacterium longum
D-galactose + ?
-
-
-
?
beta-1,3-D-galactopentaose + H2O
-
731184
Bifidobacterium longum JCM 1217
D-galactose + ?
-
-
-
?
beta-1,3-D-galactotetraose + H2O
-
731184
Bifidobacterium longum
D-galactose + ?
-
-
-
?
beta-1,3-D-galactotetraose + H2O
-
731184
Bifidobacterium longum JCM 1217
D-galactose + ?
-
-
-
?
beta-1,3-D-galactotriose + H2O
-
731184
Bifidobacterium longum
D-galactose + ?
-
-
-
?
dearabinosylated gum arabic + H2O
-
731184
Bifidobacterium longum
?
-
-
-
?
dearabinosylated larch wood arabinogalactan + H2O
best substrate
731184
Bifidobacterium longum
?
-
-
-
?
gum arabic + H2O
-
731184
Bifidobacterium longum
?
-
-
-
?
gum arabic + H2O
-
731184
Bifidobacterium longum JCM 1217
?
-
-
-
?
larch wood arabinogalactan + H2O
-
731184
Bifidobacterium longum
?
-
-
-
?
additional information
the recombinant BLLJ_1840 expressed in Escherichia coli hydrolyzes beta-1,3-linked galactooligosaccharides but not beta-1,4- and beta-1,6-linked galactooligosaccharides. The enzyme also hydrolyzes larch wood arabinogalactan, which comprises a beta-1,3-linked galactan backbone with beta-1,6-linked galactan side chains. No activity with 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-alpha-D-galactopyranoside, 4-nitrophenyl-beta-D-glucopyranoside, 4-nitrophenyl-beta-D-xylopyranoside, and 4-nitrophenyl-beta-L-arabinopyranoside, substrate specificity, and no activity with potato galactan and laminarin, overview
731184
Bifidobacterium longum
?
-
-
-
-
additional information
the recombinant BLLJ_1840 expressed in Escherichia coli hydrolyzes beta-1,3-linked galactooligosaccharides but not beta-1,4- and beta-1,6-linked galactooligosaccharides. The enzyme also hydrolyzes larch wood arabinogalactan, which comprises a beta-1,3-linked galactan backbone with beta-1,6-linked galactan side chains. No activity with 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-alpha-D-galactopyranoside, 4-nitrophenyl-beta-D-glucopyranoside, 4-nitrophenyl-beta-D-xylopyranoside, and 4-nitrophenyl-beta-L-arabinopyranoside, substrate specificity, and no activity with potato galactan and laminarin, overview
731184
Bifidobacterium longum JCM 1217
?
-
-
-
-
Synonyms
Synonyms
Commentary
Organism
BLLJ_1840
-
Bifidobacterium longum
exo-beta-1,3-galactanase
-
Bifidobacterium longum
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
40
-
-
Bifidobacterium longum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
-
-
Bifidobacterium longum
Cloned(Commentary) (protein specific)
Commentary
Organism
BLLJ_1840, phylogenetic tree, expresion as soluble enzyme in Escherichia coli
Bifidobacterium longum
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
construction of deletion mutants NDELTA42, NDELTA42CDELTA257, NDELTA42CDELTA457, and NDELTA42CDELTA76 showing 100%, 80.1%, 122%, and 8.45% activity relative to wild-type enzyme activity
Bifidobacterium longum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic parameters of enzyme BLLJ_1840 activity on different substrates, overview
Bifidobacterium longum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
106100
-
sequence calculation
Bifidobacterium longum
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.0139
-
substrate beta-1,3-D-galactotetraose, pH 5.5, 40C
Bifidobacterium longum
0.0157
-
substrate beta-1,3-D-galactopentaose, pH 5.5, 40C
Bifidobacterium longum
0.0188
-
substrate beta-1,3-D-galactotriose, pH 5.5, 40C
Bifidobacterium longum
0.0218
-
substrate beta-1,3-D-galactobiose, pH 5.5, 40C
Bifidobacterium longum
0.113
-
substrate gum arabic, pH 5.5, 40C
Bifidobacterium longum
1.01
-
substrate beta-1,3-galactan, pH 5.5, 40C
Bifidobacterium longum
1.17
-
substrate larch wood arabinogalactan, pH 5.5, 40C
Bifidobacterium longum
1.28
-
substrate dearabinosylated gum arabic, pH 5.5, 40C
Bifidobacterium longum
16.2
-
substrate dearabinosylated larch wood arabinogalactan, pH 5.5, 40C
Bifidobacterium longum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
beta-1,3-D-galactobiose + H2O
best beta-1,3-galactan substrate
731184
Bifidobacterium longum
2 D-galactose
-
-
-
?
beta-1,3-D-galactobiose + H2O
best beta-1,3-galactan substrate
731184
Bifidobacterium longum JCM 1217
2 D-galactose
-
-
-
?
beta-1,3-D-galactopentaose + H2O
-
731184
Bifidobacterium longum
D-galactose + ?
-
-
-
?
beta-1,3-D-galactopentaose + H2O
-
731184
Bifidobacterium longum JCM 1217
D-galactose + ?
-
-
-
?
beta-1,3-D-galactotetraose + H2O
-
731184
Bifidobacterium longum
D-galactose + ?
-
-
-
?
beta-1,3-D-galactotetraose + H2O
-
731184
Bifidobacterium longum JCM 1217
D-galactose + ?
-
-
-
?
beta-1,3-D-galactotriose + H2O
-
731184
Bifidobacterium longum
D-galactose + ?
-
-
-
?
dearabinosylated gum arabic + H2O
-
731184
Bifidobacterium longum
?
-
-
-
?
dearabinosylated larch wood arabinogalactan + H2O
best substrate
731184
Bifidobacterium longum
?
-
-
-
?
gum arabic + H2O
-
731184
Bifidobacterium longum
?
-
-
-
?
gum arabic + H2O
-
731184
Bifidobacterium longum JCM 1217
?
-
-
-
?
larch wood arabinogalactan + H2O
-
731184
Bifidobacterium longum
?
-
-
-
?
additional information
the recombinant BLLJ_1840 expressed in Escherichia coli hydrolyzes beta-1,3-linked galactooligosaccharides but not beta-1,4- and beta-1,6-linked galactooligosaccharides. The enzyme also hydrolyzes larch wood arabinogalactan, which comprises a beta-1,3-linked galactan backbone with beta-1,6-linked galactan side chains. No activity with 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-alpha-D-galactopyranoside, 4-nitrophenyl-beta-D-glucopyranoside, 4-nitrophenyl-beta-D-xylopyranoside, and 4-nitrophenyl-beta-L-arabinopyranoside, substrate specificity, and no activity with potato galactan and laminarin, overview
731184
Bifidobacterium longum
?
-
-
-
-
additional information
the recombinant BLLJ_1840 expressed in Escherichia coli hydrolyzes beta-1,3-linked galactooligosaccharides but not beta-1,4- and beta-1,6-linked galactooligosaccharides. The enzyme also hydrolyzes larch wood arabinogalactan, which comprises a beta-1,3-linked galactan backbone with beta-1,6-linked galactan side chains. No activity with 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-alpha-D-galactopyranoside, 4-nitrophenyl-beta-D-glucopyranoside, 4-nitrophenyl-beta-D-xylopyranoside, and 4-nitrophenyl-beta-L-arabinopyranoside, substrate specificity, and no activity with potato galactan and laminarin, overview
731184
Bifidobacterium longum JCM 1217
?
-
-
-
-
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
40
-
-
Bifidobacterium longum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
-
-
Bifidobacterium longum
Expression
Organism
Commentary
Expression
Bifidobacterium longum
the enzyme is strongly induced by larch wood arabinogalactan
up
General Information
General Information
Commentary
Organism
evolution
the enzyme belongs to the glycoside hydrolase family 43, GH43, phylogenetic relationships of BLLJ_1840
Bifidobacterium longum
additional information
domain structures of BLLJ_1840 comprising the GH43 catalytic domain, bacterial Ig-like domain, CBM32, and the LPXTG cell wall anchor domain (Anchor), as well as the signal peptide
Bifidobacterium longum
General Information (protein specific)
General Information
Commentary
Organism
evolution
the enzyme belongs to the glycoside hydrolase family 43, GH43, phylogenetic relationships of BLLJ_1840
Bifidobacterium longum
additional information
domain structures of BLLJ_1840 comprising the GH43 catalytic domain, bacterial Ig-like domain, CBM32, and the LPXTG cell wall anchor domain (Anchor), as well as the signal peptide
Bifidobacterium longum
Expression (protein specific)
Organism
Commentary
Expression
Bifidobacterium longum
the enzyme is strongly induced by larch wood arabinogalactan
up
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
752571
Fujita
Degradative enzymes for type ...
Bifidobacterium longum subsp. Longum
Appl. Microbiol. Biotechnol.
103
1299-1310
2019
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
6
1
6
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
6
1
1
-
-
-
1
-
-
-
-
3
3
-
-
-
752397
Godoy
Crystal structure of a putati ...
Bifidobacterium bifidum, Bifidobacterium bifidum NCIMB 41171, Bifidobacterium bifidum S17
Acta Crystallogr. Sect. F
72
288-293
2016
-
-
1
1
-
-
-
-
-
-
1
-
-
5
-
-
1
-
-
-
-
-
3
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
3
2
-
-
-
-
-
-
-
-
-
1
1
-
-
-
731184
Fujita
Bifidobacterium longum subsp. ...
Bifidobacterium longum, Bifidobacterium longum JCM 1217
Appl. Environ. Microbiol.
80
4577-4584
2014
-
-
1
-
1
-
-
1
-
-
1
-
-
6
-
-
-
-
-
-
9
-
14
-
2
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
9
-
14
-
1
-
-
-
1
-
-
-
1
2
2
1
-
-
731202
Okawa
Identification of an exo-beta- ...
Fusarium oxysporum 12S, Fusarium oxysporum
Appl. Microbiol. Biotechnol.
97
9685-9694
2013
-
-
1
-
-
-
-
-
-
-
-
-
-
5
-
-
1
-
-
-
1
-
12
1
2
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
12
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-
731581
Ling
An exo-beta-(1->3)-D-galactana ...
Streptomyces sp. 19(2012), Streptomyces sp.
Carbohydr. Res.
352
70-81
2012
-
1
1
-
-
-
-
-
-
-
3
4
-
6
-
-
1
-
-
-
1
-
12
1
4
1
-
-
-
2
-
-
-
-
1
-
-
2
2
-
-
-
-
-
-
-
-
-
-
3
4
-
-
-
2
-
-
2
-
12
2
2
-
-
-
2
-
-
2
-
1
2
-
-
-
732396
Jiang
Crystal structure of 1,3Gal43A ...
Hungateiclostridium thermocellum
J. Struct. Biol.
180
447-457
2012
-
-
1
1
2
-
-
-
-
-
2
-
-
4
-
-
1
-
-
-
-
-
1
2
3
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
2
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
1
2
1
-
-
-
1
-
-
-
-
2
2
-
-
-
713904
Sakamoto
Characterization of an exo-bet ...
Sphingomonas sp., Sphingomonas sp. 24T
Appl. Microbiol. Biotechnol.
90
1701-1710
2011
1
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
1
-
-
1
2
-
12
1
2
1
-
1
-
1
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
2
-
12
1
1
-
1
-
1
-
1
-
-
-
-
-
-
-
703061
Ling
Preparation of a new chromogen ...
Irpex lacteus
Carbohydr. Res.
344
1941-1946
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
706965
Ishida
Crystallization of selenomethi ...
Phanerochaete chrysosporium
Acta Crystallogr. Sect. F
65
1274-1276
2009
-
-
1
1
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
1
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
707784
Kotake
Molecular cloning and expressi ...
Irpex lacteus, Irpex lacteus NBRC 5367
Biosci. Biotechnol. Biochem.
73
2303-2309
2009
-
-
1
-
-
-
-
-
-
-
1
-
-
2
-
-
1
-
-
-
1
-
4
1
2
1
1
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
4
1
1
1
-
-
1
1
-
-
-
-
-
-
-
-
677662
Ichinose
Characterization of an exo-bet ...
Hungateiclostridium thermocellum
Appl. Environ. Microbiol.
72
3515-3523
2006
-
-
1
-
-
-
-
-
-
-
1
-
-
4
-
-
1
-
-
-
1
-
5
-
2
2
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
5
-
2
-
-
-
1
1
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