Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.145 extracted from

  • Kotake, T.; Kitazawa, K.; Takata, R.; Okabe, K.; Ichingse, H.; Kaneko, S.; Tsumuraya, Y.
    Molecular cloning and expression in Pichia pastoris of a Irpex lacteus exo-/beta-1,3-galactanase gene (2009), Biosci. Biotechnol. Biochem., 73, 2303-2309.
    View publication on PubMed
Search for more literature for 3.2.1.145

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, expression of the gene encoding the enzyme fused to a yeast secretion signal sequence under control of the alcohol oxidase promoter in Pichia pastoris Irpex lacteus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45520
-
 x * 45520, mature enzyme, sequence calculation Irpex lacteus

Organism

Organism UniProt Comment Textmining
Irpex lacteus
-
-
-
Irpex lacteus NBRC 5367
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme 0.7fold from Pichia pastoris cell culture medium by cation exchange chromatography and gel filtration Irpex lacteus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
47.6
-
 purified recombinant enzyme Irpex lacteus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-1,3-galactan + H2O best substrate, mode of action, overview Irpex lacteus ?
-
 ?
beta-1,3-galactan + H2O best substrate, mode of action, overview Irpex lacteus NBRC 5367 ?
-
 ?
additional information substrate specificity, the recombinant enzyme specifically hydrolyses beta-1,3-galactooligosacchrides, as an exo-beta-1,3-galactanase, with high activity for arabinogalactan-proteins from radish, hydrolyzing beta-1,3-galactan main chains, as well as beta-1,3-galactan. Products are beta-1,6-galactobiose, beta-1,6-galactotriose, alpha-arabinofuranosyl-1,3-galactosyl-beta-1,6-galatose with galactose from beta-1,3-galactans attached with and without beta-1,6-galatosyl branchesprepared from acacia gum, overview Irpex lacteus ?
-
 ?
additional information substrate specificity, the recombinant enzyme specifically hydrolyses beta-1,3-galactooligosacchrides, as an exo-beta-1,3-galactanase, with high activity for arabinogalactan-proteins from radish, hydrolyzing beta-1,3-galactan main chains, as well as beta-1,3-galactan. Products are beta-1,6-galactobiose, beta-1,6-galactotriose, alpha-arabinofuranosyl-1,3-galactosyl-beta-1,6-galatose with galactose from beta-1,3-galactans attached with and without beta-1,6-galatosyl branchesprepared from acacia gum, overview Irpex lacteus NBRC 5367 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 45520, mature enzyme, sequence calculation Irpex lacteus

Synonyms

Synonyms Comment Organism
exo-/beta-1,3-galactanase
-
 Irpex lacteus
Il1,3Gal
-
 Irpex lacteus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 recombinant enzyme Irpex lacteus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 55 activity range, temperature profile, recombinant enzyme, overview Irpex lacteus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
 recombinant enzyme Irpex lacteus

pH Range

pH Minimum pH Maximum Comment Organism
3 6.5 activity range, pH profile, recombinant enzyme, overview Irpex lacteus