BRENDA - Enzyme Database
show all sequences of 3.2.1.145

Crystallization of selenomethionyl exo-beta-1,3-galactanase from the basidiomycete Phanerochaete chrysosporium

Ishida, T.; Fujimoto, Z.; Ichinose, H.; Igarashi, K.; Kaneko, S.; Samejima, M.; Acta Crystallogr. Sect. F 65, 1274-1276 (2009)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
Pc1,3Gal43A, expression in Pichia pastoris as selenmethionine-labeled protein
Phanerochaete chrysosporium
Crystallization (Commentary)
Crystallization (Commentary)
Organism
selenomethionine-labeled recombinant Pc1,3Gal43A, hanging-drop vapour-diffusion method, 25°C, the reservoir solution contains 16% w/v PEG 10000, 95 mM ammonium sulfate, 95 mM Bis-Tris, pH 5.5, and 4.8% v/v glycerol, 3-10 days, X-ray diffraction structure determination and analysis at 1.8 A resolution, multiwavelength anomalous dispersion method, since molecular replacement is unsuccessful
Phanerochaete chrysosporium
Organism
Organism
UniProt
Commentary
Textmining
Phanerochaete chrysosporium
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
recombinant selenmethionine-labeled Pc1,3Gal43A from Pichia pastoris by ultrafiltration, and hydrophobic interaction chromatography
Phanerochaete chrysosporium
Subunits
Subunits
Commentary
Organism
More
the enzyme consists of a glycoside hydrolase family 43 catalytic domain and a substrate-binding domain that belongs to carbohydrate-binding module family 35
Phanerochaete chrysosporium
Synonyms
Synonyms
Commentary
Organism
exo-beta-1,3-galactanase
-
Phanerochaete chrysosporium
More
the enzyme consists of a glycoside hydrolase family 43 catalytic domain and a substrate-binding domain that belongs to carbohydrate-binding module family 35
Phanerochaete chrysosporium
Pc1,3Gal43A
-
Phanerochaete chrysosporium
Cloned(Commentary) (protein specific)
Commentary
Organism
Pc1,3Gal43A, expression in Pichia pastoris as selenmethionine-labeled protein
Phanerochaete chrysosporium
Crystallization (Commentary) (protein specific)
Crystallization
Organism
selenomethionine-labeled recombinant Pc1,3Gal43A, hanging-drop vapour-diffusion method, 25°C, the reservoir solution contains 16% w/v PEG 10000, 95 mM ammonium sulfate, 95 mM Bis-Tris, pH 5.5, and 4.8% v/v glycerol, 3-10 days, X-ray diffraction structure determination and analysis at 1.8 A resolution, multiwavelength anomalous dispersion method, since molecular replacement is unsuccessful
Phanerochaete chrysosporium
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant selenmethionine-labeled Pc1,3Gal43A from Pichia pastoris by ultrafiltration, and hydrophobic interaction chromatography
Phanerochaete chrysosporium
Subunits (protein specific)
Subunits
Commentary
Organism
More
the enzyme consists of a glycoside hydrolase family 43 catalytic domain and a substrate-binding domain that belongs to carbohydrate-binding module family 35
Phanerochaete chrysosporium
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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-
-
-
-
-
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3
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6
1
6
1
-
-
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1
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-
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1
-
-
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1
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1
1
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1
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1
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1
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3
2
2
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1
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1
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1
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1
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3
2
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1
1
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1
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1
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1
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6
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9
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14
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2
1
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1
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1
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1
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1
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1
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9
-
14
-
1
-
-
-
1
-
-
-
1
2
2
1
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731202
Okawa
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97
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1
-
-
-
-
-
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5
-
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1
-
-
-
1
-
12
1
2
1
-
-
-
1
-
-
-
-
-
-
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1
-
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-
-
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1
-
-
1
-
12
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-
731581
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An exo-beta-(1->3)-D-galactana ...
Streptomyces sp. 19(2012), Streptomyces sp.
Carbohydr. Res.
352
70-81
2012
-
1
1
-
-
-
-
-
-
-
3
4
-
6
-
-
1
-
-
-
1
-
12
1
4
1
-
-
-
2
-
-
-
-
1
-
-
2
2
-
-
-
-
-
-
-
-
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3
4
-
-
-
2
-
-
2
-
12
2
2
-
-
-
2
-
-
2
-
1
2
-
-
-
732396
Jiang
Crystal structure of 1,3Gal43A ...
Hungateiclostridium thermocellum
J. Struct. Biol.
180
447-457
2012
-
-
1
1
2
-
-
-
-
-
2
-
-
4
-
-
1
-
-
-
-
-
1
2
3
1
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1
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1
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1
2
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2
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1
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-
1
2
1
-
-
-
1
-
-
-
-
2
2
-
-
-
713904
Sakamoto
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Appl. Microbiol. Biotechnol.
90
1701-1710
2011
1
-
-
-
-
-
-
-
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1
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-
3
-
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1
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-
1
2
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12
1
2
1
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1
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1
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1
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1
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1
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1
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1
2
-
12
1
1
-
1
-
1
-
1
-
-
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-
-
-
703061
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Preparation of a new chromogen ...
Irpex lacteus
Carbohydr. Res.
344
1941-1946
2009
-
-
-
-
-
-
-
-
-
-
-
-
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1
-
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1
1
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1
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2
1
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1
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-
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1
1
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
706965
Ishida
Crystallization of selenomethi ...
Phanerochaete chrysosporium
Acta Crystallogr. Sect. F
65
1274-1276
2009
-
-
1
1
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
1
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
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-
1
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-
-
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1
-
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-
-
-
-
-
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-
-
-
-
-
707784
Kotake
Molecular cloning and expressi ...
Irpex lacteus, Irpex lacteus NBRC 5367
Biosci. Biotechnol. Biochem.
73
2303-2309
2009
-
-
1
-
-
-
-
-
-
-
1
-
-
2
-
-
1
-
-
-
1
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4
1
2
1
1
-
-
1
1
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1
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-
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1
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1
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-
1
-
4
1
1
1
-
-
1
1
-
-
-
-
-
-
-
-
677662
Ichinose
Characterization of an exo-bet ...
Hungateiclostridium thermocellum
Appl. Environ. Microbiol.
72
3515-3523
2006
-
-
1
-
-
-
-
-
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1
-
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4
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-
1
-
-
-
1
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5
-
2
2
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1
1
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1
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1
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1
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1
-
5
-
2
-
-
-
1
1
-
-
-
-
-
-
-
-
678786
Ichinose
Characterization of an exo-bet ...
Streptomyces avermitilis, Streptomyces avermitilis NBRC 14893
Biosci. Biotechnol. Biochem.
70
2745-2750
2006
-
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1
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-
-
-
-
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1
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4
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1
1
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1
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10
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2
1
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1
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1
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1
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1
1
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1
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10
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1
-
-
-
1
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-
-
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666634
Kotake
Molecular cloning of a b-galac ...
Raphanus sativus
Plant Physiol.
138
1563-1576
2005
-
-
1
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4
3
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2
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1
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1
-
10
1
1
1
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1
3
1
1
-
-
-
-
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-
1
-
-
-
-
-
4
-
3
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2
-
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-
1
-
-
1
-
10
1
1
-
1
3
1
1
-
-
-
-
-
-
-
-
171335
Tsumuraya
Purification of an exo-beta-(1 ...
Irpex lacteus
J. Biol. Chem.
265
7207-7215
1990
-
-
-
-
-
-
-
2
-
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2
1
-
1
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1
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1
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4
1
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1
1
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1
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1
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2
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2
1
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1
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1
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4
1
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1
1
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1
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1
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