BRENDA - Enzyme Database
show all sequences of 3.2.1.145

Purification of an exo-beta-(1->3)-D-galactanase of Irpex lacteus (Polyporus tulipiferae) and its action on arabinogalactan-proteins

Tsumuraya, Y.; Mochizuki, N.; Hashimoto, Y.; Kovac, P.; J. Biol. Chem. 265, 7207-7215 (1990)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
19.9
-
beta-(1-3)-D-galactotriose
-
Irpex lacteus
31.6
-
beta-(1-3)-D-galactobiose
-
Irpex lacteus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42000
-
gel filtration
Irpex lacteus
51000
-
native PAGE
Irpex lacteus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
beta-(1-3)-D-galactan + H2O
Irpex lacteus
-
beta-(1-3)-D-galactan + D-galactose
-
Irpex lacteus
?
Organism
Organism
UniProt
Commentary
Textmining
Irpex lacteus
-
Polyporus tulipiferae
-
Purification (Commentary)
Purification (Commentary)
Organism
DEAE-cellulose, CM-Toyopearl, hydroylapatite, electrofocusing, purified from commercial driselase
Irpex lacteus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
87.8
-
-
Irpex lacteus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
beta-(1-3)-D-galactan + H2O
-
171335
Irpex lacteus
beta-(1-3)-D-galactan + D-galactose
-
171335
Irpex lacteus
?
beta-(1-3)-D-galactotriose + H2O
-
171335
Irpex lacteus
beta-(1-3)-D-galactobiose + D-galactose
-
-
-
?
beta-(1-3)-galactobiose + H2O
-
171335
Irpex lacteus
D-galactose
-
171335
Irpex lacteus
?
beta-D-Gal-[(1->3)-beta-D-Gal]n + H2O
only beta-(1, 3) oligomers act as substrates
171335
Irpex lacteus
beta-D-Gal-[(1->3)-beta-D-Gal]n-1 + D-galactose
-
171335
Irpex lacteus
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 51000, SDS-PAGE
Irpex lacteus
Temperature Range [C]
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
30
48
retains full activity up to 48C, completely inactivated at 60C
Irpex lacteus
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
30
48
retains full activity up to 48C, completely inactivated at 60C
Irpex lacteus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.6
-
enzyme action on beta-(1-3)-D-galactan
Irpex lacteus
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
3.5
7.5
after incubation for 24 h at 4C
Irpex lacteus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
19.9
-
beta-(1-3)-D-galactotriose
-
Irpex lacteus
31.6
-
beta-(1-3)-D-galactobiose
-
Irpex lacteus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42000
-
gel filtration
Irpex lacteus
51000
-
native PAGE
Irpex lacteus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
beta-(1-3)-D-galactan + H2O
Irpex lacteus
-
beta-(1-3)-D-galactan + D-galactose
-
Irpex lacteus
?
Purification (Commentary) (protein specific)
Commentary
Organism
DEAE-cellulose, CM-Toyopearl, hydroylapatite, electrofocusing, purified from commercial driselase
Irpex lacteus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
87.8
-
-
Irpex lacteus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
beta-(1-3)-D-galactan + H2O
-
171335
Irpex lacteus
beta-(1-3)-D-galactan + D-galactose
-
171335
Irpex lacteus
?
beta-(1-3)-D-galactotriose + H2O
-
171335
Irpex lacteus
beta-(1-3)-D-galactobiose + D-galactose
-
-
-
?
beta-(1-3)-galactobiose + H2O
-
171335
Irpex lacteus
D-galactose
-
171335
Irpex lacteus
?
beta-D-Gal-[(1->3)-beta-D-Gal]n + H2O
only beta-(1, 3) oligomers act as substrates
171335
Irpex lacteus
beta-D-Gal-[(1->3)-beta-D-Gal]n-1 + D-galactose
-
171335
Irpex lacteus
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 51000, SDS-PAGE
Irpex lacteus
Temperature Range [C] (protein specific)
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
30
48
retains full activity up to 48C, completely inactivated at 60C
Irpex lacteus
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
30
48
retains full activity up to 48C, completely inactivated at 60C
Irpex lacteus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.6
-
enzyme action on beta-(1-3)-D-galactan
Irpex lacteus
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
3.5
7.5
after incubation for 24 h at 4C
Irpex lacteus
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
752571
Fujita
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103
1299-1310
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-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
6
1
6
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
6
1
1
-
-
-
1
-
-
-
-
3
3
-
-
-
752397
Godoy
Crystal structure of a putati ...
Bifidobacterium bifidum, Bifidobacterium bifidum NCIMB 41171, Bifidobacterium bifidum S17
Acta Crystallogr. Sect. F
72
288-293
2016
-
-
1
1
-
-
-
-
-
-
1
-
-
5
-
-
1
-
-
-
-
-
3
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
3
2
-
-
-
-
-
-
-
-
-
1
1
-
-
-
731184
Fujita
Bifidobacterium longum subsp. ...
Bifidobacterium longum, Bifidobacterium longum JCM 1217
Appl. Environ. Microbiol.
80
4577-4584
2014
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-
1
-
1
-
-
1
-
-
1
-
-
6
-
-
-
-
-
-
9
-
14
-
2
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
9
-
14
-
1
-
-
-
1
-
-
-
1
2
2
1
-
-
731202
Okawa
Identification of an exo-beta- ...
Fusarium oxysporum 12S, Fusarium oxysporum
Appl. Microbiol. Biotechnol.
97
9685-9694
2013
-
-
1
-
-
-
-
-
-
-
-
-
-
5
-
-
1
-
-
-
1
-
12
1
2
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
12
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-
731581
Ling
An exo-beta-(1->3)-D-galactana ...
Streptomyces sp. 19(2012), Streptomyces sp.
Carbohydr. Res.
352
70-81
2012
-
1
1
-
-
-
-
-
-
-
3
4
-
6
-
-
1
-
-
-
1
-
12
1
4
1
-
-
-
2
-
-
-
-
1
-
-
2
2
-
-
-
-
-
-
-
-
-
-
3
4
-
-
-
2
-
-
2
-
12
2
2
-
-
-
2
-
-
2
-
1
2
-
-
-
732396
Jiang
Crystal structure of 1,3Gal43A ...
Hungateiclostridium thermocellum
J. Struct. Biol.
180
447-457
2012
-
-
1
1
2
-
-
-
-
-
2
-
-
4
-
-
1
-
-
-
-
-
1
2
3
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
2
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
1
2
1
-
-
-
1
-
-
-
-
2
2
-
-
-
713904
Sakamoto
Characterization of an exo-bet ...
Sphingomonas sp., Sphingomonas sp. 24T
Appl. Microbiol. Biotechnol.
90
1701-1710
2011
1
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
1
-
-
1
2
-
12
1
2
1
-
1
-
1
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
2
-
12
1
1
-
1
-
1
-
1
-
-
-
-
-
-
-
703061
Ling
Preparation of a new chromogen ...
Irpex lacteus
Carbohydr. Res.
344
1941-1946
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
706965
Ishida
Crystallization of selenomethi ...
Phanerochaete chrysosporium
Acta Crystallogr. Sect. F
65
1274-1276
2009
-
-
1
1
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
1
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
707784
Kotake
Molecular cloning and expressi ...
Irpex lacteus, Irpex lacteus NBRC 5367
Biosci. Biotechnol. Biochem.
73
2303-2309
2009
-
-
1
-
-
-
-
-
-
-
1
-
-
2
-
-
1
-
-
-
1
-
4
1
2
1
1
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
4
1
1
1
-
-
1
1
-
-
-
-
-
-
-
-
677662
Ichinose
Characterization of an exo-bet ...
Hungateiclostridium thermocellum
Appl. Environ. Microbiol.
72
3515-3523
2006
-
-
1
-
-
-
-
-
-
-
1
-
-
4
-
-
1
-
-
-
1
-
5
-
2
2
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
5
-
2
-
-
-
1
1
-
-
-
-
-
-
-
-
678786
Ichinose
Characterization of an exo-bet ...
Streptomyces avermitilis, Streptomyces avermitilis NBRC 14893
Biosci. Biotechnol. Biochem.
70
2745-2750
2006
-
-
1
-
-
-
-
-
-
-
1
-
-
4
-
1
1
-
-
-
1
-
10
-
2
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
1
-
-
1
-
10
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
666634
Kotake
Molecular cloning of a b-galac ...
Raphanus sativus
Plant Physiol.
138
1563-1576
2005
-
-
1
-
-
-
4
3
-
-
2
-
-
4
-
-
1
-
-
-
1
-
10
1
1
1
-
1
3
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
4
-
3
-
-
2
-
-
-
-
1
-
-
1
-
10
1
1
-
1
3
1
1
-
-
-
-
-
-
-
-
171335
Tsumuraya
Purification of an exo-beta-(1 ...
Irpex lacteus
J. Biol. Chem.
265
7207-7215
1990
-
-
-
-
-
-
-
2
-
-
2
1
-
1
-
-
1
-
-
-
1
-
4
1
-
-
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
2
1
-
-
-
1
-
-
1
-
4
1
-
1
1
-
1
-
1
-
-
-
-
-
-
-