BRENDA - Reference to 3.2.1.145; Id = 731581

analysis

the activity of the enzyme is well suited for the study of type II galactan structures and provides an important tool for the investigation of the biological role of arabinogalactan proteins in plants; the activity of the enzyme is well suited for the study of type II galactan structures and provides an important tool for the investigation of the biological role of arabinogalactan proteins in plants

Streptomyces sp.

SGalase1, DNA and amino acid equence determination and analysis, sequence comparison, phylogenetic analysis and tree; SGalase2, DNA and amino acid equence determination and analysis, sequence comparison, phylogenetic analysis and tree

Streptomyces sp.

45000

-

x * 45000, about, SDS-PAGE, x * 48200, about, sequence calculation

Streptomyces sp.

47900

-

x * 47900, about, sequence calculation

Streptomyces sp.

48200

-

x * 45000, about, SDS-PAGE, x * 48200, about, sequence calculation

Streptomyces sp.

additional information

Streptomyces sp.

the enzyme specifically cleaves the beta-(1->3)-D-galactan backbone of arabinogalactan-proteins

?

-

additional information

Streptomyces sp. 19(2012)

the enzyme specifically cleaves the beta-(1->3)-D-galactan backbone of arabinogalactan-proteins

?

-

Streptomyces sp.

I0B0S9

SGalase1; isolated from culture filtrates of soil

-

Streptomyces sp.

I0B0T0

SGalase2; isolated from culture filtrates of soil

-

Streptomyces sp. 19(2012)

I0B0S9

SGalase1; isolated from culture filtrates of soil

-

Streptomyces sp. 19(2012)

I0B0T0

SGalase2; isolated from culture filtrates of soil

-

native enzyme 1.8fold from culture supernatant by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration; native enzyme 1.8fold from culture supernatant by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration

Streptomyces sp.

40

-

purified native enzyme, pH 3.8, 48°C; purified native enzyme, pH 5.0, 48°C

Streptomyces sp.

dearabinosylated gum arabic + H2O

de-arabinosylated gum arabic is used as a model to investigate the use of the enzyme in defining type II galactan structure, kinetics, overview. Exhaustive hydrolysis of dearabinosylated gum arabic resultes in a limited number of oligosaccharide products with a trisaccharide of Gal2GlcA1 predominating, mass spectrometric analysis

731581

Streptomyces sp.

?

-

?

dearabinosylated gum arabic + H2O

de-arabinosylated gum arabic is used as a model to investigate the use of the enzyme in defining type II galactan structure, kinetics, overview. Exhaustive hydrolysis of dearabinosylated gum arabic resultes in a limited number of oligosaccharide products with a trisaccharide of Gal2GlcA1 predominating., mass spectrometric analysis

731581

Streptomyces sp.

?

-

?

dearabinosylated gum arabic + H2O

de-arabinosylated gum arabic is used as a model to investigate the use of the enzyme in defining type II galactan structure, kinetics, overview. Exhaustive hydrolysis of dearabinosylated gum arabic resultes in a limited number of oligosaccharide products with a trisaccharide of Gal2GlcA1 predominating., mass spectrometric analysis

731581

Streptomyces sp. 19(2012)

?

-

?

dearabinosylated gum arabic + H2O

de-arabinosylated gum arabic is used as a model to investigate the use of the enzyme in defining type II galactan structure, kinetics, overview. Exhaustive hydrolysis of dearabinosylated gum arabic resultes in a limited number of oligosaccharide products with a trisaccharide of Gal2GlcA1 predominating, mass spectrometric analysis

731581

Streptomyces sp. 19(2012)

?

-

?

additional information

the enzyme specifically cleaves the beta-(1->3)-D-galactan backbone of arabinogalactan-proteins

731581

Streptomyces sp.

?

-

additional information

the enzyme specifically hydrolyses beta-(1->3)-D-galacto-oligo- or poly-saccharides from the upstream (non-reducing) end, typical of an exo-acting enzyme. Substrate specificity, overview. No or negligible activity on 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl beta-D-fucopyranoside,4-nitrophenyl alpha-L-arabinofuranosidase 4-nitrophenyl alpha-D-galactopyranoside, 4-nitrophenyl alpha/beta-D-glucopyranoside, 4-nitrophenyl alpha/beta-L-fucopyranoside, 4-nitrophenyl beta-D-cellobioside, 4-nitrophenyl alpha-D-mannopyranoside, or 4-nitrophenyl beta-D-xylopyranoside

731581

Streptomyces sp.

?

-

additional information

the enzyme specifically cleaves the beta-(1->3)-D-galactan backbone of arabinogalactan-proteins

731581

Streptomyces sp. 19(2012)

?

-

additional information

the enzyme specifically hydrolyses beta-(1->3)-D-galacto-oligo- or poly-saccharides from the upstream (non-reducing) end, typical of an exo-acting enzyme. Substrate specificity, overview. No or negligible activity on 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl beta-D-fucopyranoside,4-nitrophenyl alpha-L-arabinofuranosidase 4-nitrophenyl alpha-D-galactopyranoside, 4-nitrophenyl alpha/beta-D-glucopyranoside, 4-nitrophenyl alpha/beta-L-fucopyranoside, 4-nitrophenyl beta-D-cellobioside, 4-nitrophenyl alpha-D-mannopyranoside, or 4-nitrophenyl beta-D-xylopyranoside

731581

Streptomyces sp. 19(2012)

?

-

?

x * 45000, about, SDS-PAGE, x * 48200, about, sequence calculation; x * 47900, about, sequence calculation

Streptomyces sp.

exo-beta-(1->3)-D-galactanase

-

Streptomyces sp.

SGalase1

-

Streptomyces sp.

SGalase2

-

Streptomyces sp.

48

-

;

Streptomyces sp.

3.8

-

Streptomyces sp.

5

-

Streptomyces sp.

about, sequence calculation; about, sequence calculation

-

7.45

analysis

the activity of the enzyme is well suited for the study of type II galactan structures and provides an important tool for the investigation of the biological role of arabinogalactan proteins in plants

Streptomyces sp.

SGalase1, DNA and amino acid equence determination and analysis, sequence comparison, phylogenetic analysis and tree

Streptomyces sp.

SGalase2, DNA and amino acid equence determination and analysis, sequence comparison, phylogenetic analysis and tree

Streptomyces sp.

45000

-

x * 45000, about, SDS-PAGE, x * 48200, about, sequence calculation

Streptomyces sp.

47900

-

x * 47900, about, sequence calculation

Streptomyces sp.

48200

-

x * 45000, about, SDS-PAGE, x * 48200, about, sequence calculation

Streptomyces sp.

additional information

Streptomyces sp.

the enzyme specifically cleaves the beta-(1->3)-D-galactan backbone of arabinogalactan-proteins

?

-

additional information

Streptomyces sp. 19(2012)

the enzyme specifically cleaves the beta-(1->3)-D-galactan backbone of arabinogalactan-proteins

?

-

native enzyme 1.8fold from culture supernatant by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration

Streptomyces sp.

40

-

purified native enzyme, pH 3.8, 48°C

Streptomyces sp.

40

-

purified native enzyme, pH 5.0, 48°C

Streptomyces sp.

dearabinosylated gum arabic + H2O

de-arabinosylated gum arabic is used as a model to investigate the use of the enzyme in defining type II galactan structure, kinetics, overview. Exhaustive hydrolysis of dearabinosylated gum arabic resultes in a limited number of oligosaccharide products with a trisaccharide of Gal2GlcA1 predominating, mass spectrometric analysis

731581

Streptomyces sp.

?

-

?

dearabinosylated gum arabic + H2O

de-arabinosylated gum arabic is used as a model to investigate the use of the enzyme in defining type II galactan structure, kinetics, overview. Exhaustive hydrolysis of dearabinosylated gum arabic resultes in a limited number of oligosaccharide products with a trisaccharide of Gal2GlcA1 predominating., mass spectrometric analysis

731581

Streptomyces sp.

?

-

?

dearabinosylated gum arabic + H2O

de-arabinosylated gum arabic is used as a model to investigate the use of the enzyme in defining type II galactan structure, kinetics, overview. Exhaustive hydrolysis of dearabinosylated gum arabic resultes in a limited number of oligosaccharide products with a trisaccharide of Gal2GlcA1 predominating., mass spectrometric analysis

731581

Streptomyces sp. 19(2012)

?

-

?

dearabinosylated gum arabic + H2O

de-arabinosylated gum arabic is used as a model to investigate the use of the enzyme in defining type II galactan structure, kinetics, overview. Exhaustive hydrolysis of dearabinosylated gum arabic resultes in a limited number of oligosaccharide products with a trisaccharide of Gal2GlcA1 predominating, mass spectrometric analysis

731581

Streptomyces sp. 19(2012)

?

-

?

additional information

the enzyme specifically cleaves the beta-(1->3)-D-galactan backbone of arabinogalactan-proteins

731581

Streptomyces sp.

?

-

additional information

the enzyme specifically hydrolyses beta-(1->3)-D-galacto-oligo- or poly-saccharides from the upstream (non-reducing) end, typical of an exo-acting enzyme. Substrate specificity, overview. No or negligible activity on 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl beta-D-fucopyranoside,4-nitrophenyl alpha-L-arabinofuranosidase 4-nitrophenyl alpha-D-galactopyranoside, 4-nitrophenyl alpha/beta-D-glucopyranoside, 4-nitrophenyl alpha/beta-L-fucopyranoside, 4-nitrophenyl beta-D-cellobioside, 4-nitrophenyl alpha-D-mannopyranoside, or 4-nitrophenyl beta-D-xylopyranoside

731581

Streptomyces sp.

?

-

additional information

the enzyme specifically cleaves the beta-(1->3)-D-galactan backbone of arabinogalactan-proteins

731581

Streptomyces sp. 19(2012)

?

-

additional information

the enzyme specifically hydrolyses beta-(1->3)-D-galacto-oligo- or poly-saccharides from the upstream (non-reducing) end, typical of an exo-acting enzyme. Substrate specificity, overview. No or negligible activity on 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl beta-D-fucopyranoside,4-nitrophenyl alpha-L-arabinofuranosidase 4-nitrophenyl alpha-D-galactopyranoside, 4-nitrophenyl alpha/beta-D-glucopyranoside, 4-nitrophenyl alpha/beta-L-fucopyranoside, 4-nitrophenyl beta-D-cellobioside, 4-nitrophenyl alpha-D-mannopyranoside, or 4-nitrophenyl beta-D-xylopyranoside

731581

Streptomyces sp. 19(2012)

?

-

?

x * 45000, about, SDS-PAGE, x * 48200, about, sequence calculation

Streptomyces sp.

?

x * 47900, about, sequence calculation

Streptomyces sp.

48

-

Streptomyces sp.

3.8

-

Streptomyces sp.

5

-

Streptomyces sp.

about, sequence calculation

-

7.45

evolution

the enzyme belongs to the glycoside hydrolase family 43, GH43, phylogenetic analysis and tree; the enzyme belongs to the glycoside hydrolase family 43, GH43, phylogenetic analysis and tree

Streptomyces sp.

evolution

the enzyme belongs to the glycoside hydrolase family 43, GH43, phylogenetic analysis and tree

Streptomyces sp.

752571

Fujita

Degradative enzymes for type ...

Bifidobacterium longum subsp. Longum

Appl. Microbiol. Biotechnol.

103

1299-1310

2019

-

1

-

3

-

1

-

6

1

6

1

-

1

-

1

-

1

-

6

1

-

1

-

3

-

752397

Godoy

Crystal structure of a putati ...

Bifidobacterium bifidum, Bifidobacterium bifidum NCIMB 41171, Bifidobacterium bifidum S17

Acta Crystallogr. Sect. F

72

288-293

2016

-

1

-

1

-

5

-

1

-

3

2

-

1

-

1

-

1

-

1

-

3

2

-

1

-

731184

Fujita

Bifidobacterium longum subsp. ...

Bifidobacterium longum, Bifidobacterium longum JCM 1217

Appl. Environ. Microbiol.

80

4577-4584

2014

-

1

-

1

-

1

-

1

-

6

-

9

-

14

-

2

1

-

1

-

1

-

1

-

1

-

1

-

9

-

14

-

1

-

1

-

1

2

1

-

731202

Okawa

Identification of an exo-beta- ...

Fusarium oxysporum 12S, Fusarium oxysporum

Appl. Microbiol. Biotechnol.

97

9685-9694

2013

-

1

-

5

-

1

-

1

-

12

1

2

1

-

1

-

1

-

1

-

1

-

12

1

-

1

-

1

-

731581

Ling

An exo-beta-(1->3)-D-galactana ...

Streptomyces sp. 19(2012), Streptomyces sp.

Carbohydr. Res.

352

70-81

2012

-

1

-

3

4

-

6

-

1

-

1

-

12

1

4

1

-

2

-

1

-

2

-

3

4

-

2

-

2

-

12

2

-

2

-

2

-

1

2

-

732396

Jiang

Crystal structure of 1,3Gal43A ...

Hungateiclostridium thermocellum

J. Struct. Biol.

180

447-457

2012

-

1

2

-

2

-

4

-

1

-

1

2

3

1

-

1

-

1

-

1

2

-

2

-

1

-

1

2

1

-

1

-

2

-

713904

Sakamoto

Characterization of an exo-bet ...

Sphingomonas sp., Sphingomonas sp. 24T

Appl. Microbiol. Biotechnol.

90

1701-1710

2011

1

-

1

-

3

-

1

-

1

2

-

12

1

2

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

2

-

12

1

-

1

-

1

-

1

-

703061

Ling

Preparation of a new chromogen ...

Irpex lacteus

Carbohydr. Res.

344

1941-1946

2009

-

1

-

1

-

1

-

2

1

-

1

-

1

-

1

-

1

-

1

-

706965

Ishida

Crystallization of selenomethi ...

Phanerochaete chrysosporium

Acta Crystallogr. Sect. F

65

1274-1276

2009

-

1

-

2

-

1

-

1

3

-

1

-

1

-

1

-

1

-

707784

Kotake

Molecular cloning and expressi ...

Irpex lacteus, Irpex lacteus NBRC 5367

Biosci. Biotechnol. Biochem.

73

2303-2309

2009

-

1

-

1

-

2

-

1

-

1

-

4

1

2

1

-

1

-

1

-

1

-

1

-

1

-

4

1

-

1

-

677662

Ichinose

Characterization of an exo-bet ...

Hungateiclostridium thermocellum

Appl. Environ. Microbiol.

72

3515-3523

2006

-

1

-

1

-

4

-

1

-

1

-

5

-

2

-

1

-

1

-

1

-

1

-

1

-

5

-

2

-

1

-

678786

Ichinose

Characterization of an exo-bet ...

Streptomyces avermitilis, Streptomyces avermitilis NBRC 14893

Biosci. Biotechnol. Biochem.

70

2745-2750

2006

-

1

-

1

-

4

-

1

-

1

-

10

-

2

1

-

1

-

1

-

1

-

1

-

1

-

10

-

1

-

1

-

666634

Kotake

Molecular cloning of a b-galac ...

Raphanus sativus

Plant Physiol.

138

1563-1576

2005

-

1

-

4

3

-

2

-

4

-

1

-

1

-

10

1

-

1

3

1

-

1

-

4

-

3

-

2

-

1

-

1

-

10

1

-

1

3

1

-

171335

Tsumuraya

Purification of an exo-beta-(1 ...

Irpex lacteus

J. Biol. Chem.

265

7207-7215

1990

-

2

-

2

1

-

1

-

1

-

1

-

4

1

-

1

-

1

-

1

-

2

-

2

1

-

1

-

1

-

4

1

-

1

-

1

-

1

-

An exo-beta-(1->3)-D-galactanase from Streptomyces sp. provides insights into type II arabinogalactan structure

Data extracted from this reference: