BRENDA - Reference to 3.2.1.145; Id = 677662

expressed in Escherichia coli

Hungateiclostridium thermocellum

38000

-

SDS-PAGE

Hungateiclostridium thermocellum

A3DD67

precursor

-

Ni-nitrilotriacetic acid agarose colum

Hungateiclostridium thermocellum

21.3

-

beta-1,3-galactan as a substrate

Hungateiclostridium thermocellum

alpha-L-arabinofuranosidase-treated root arabinogalactan proteins from radish + H2O

relative activity: 9.3

677662

Hungateiclostridium thermocellum

?

-

?

beta-(1-3)-D-galactotriose + H2O

relative activity: 119%. Enzyme hydrolyzes beta-1,3-linked galacto-oligosaccharides but does not hydrolyze beta-1,4-linked and beta-1,6-linked galacto-oligosaccharide. Ct1,3Gal43A also can not hydrolyze the beta-1,3-galactosyl linkage of beta-1,3-galactosyl galactosaminide, beta-1,3-galactosyl glucosaminide, or beta-1,3-galactosyl L-arabinofuranoside

677662

Hungateiclostridium thermocellum

beta-(1-3)-D-galactobiose + D-galactose

-

?

beta-(1-3)-galactobiose + H2O

relative activity: 31%

677662

Hungateiclostridium thermocellum

D-galactose

-

?

beta-1,3-beta-1,6-galactan + H2O

relative activity: 3.1%

677662

Hungateiclostridium thermocellum

?

-

?

beta-1,3-galactan + H2O

relative activity: 100%. Enzyme catalyzes hydrolysis only of beta-1,3-linked galactosyl oligosaccharides and polysaccharides. Enzyme does not hydrolyze beta-1,4-linked galactose-containing polysaccharides, such as pectic galactan and alpha-L-arabinofuranosidase-treated pectic galactan from lupine, and also does not hydrolyze laminarin, CM-curdlan, or beta-1,3-xylan, suggesting that the enzyme acts only on a beta-1,3-linked galactan chain

677662

Hungateiclostridium thermocellum

oligo-beta-(1->3)-D-galactose + D-galactose

-

?

Ct1,3Gal43A

-

Hungateiclostridium thermocellum

exo-beta-1,3-D-galactanase

-

Hungateiclostridium thermocellum

37

-

assay at

Hungateiclostridium thermocellum

50

-

Hungateiclostridium thermocellum

6

-

Hungateiclostridium thermocellum

3

10

-

Hungateiclostridium thermocellum

expressed in Escherichia coli

Hungateiclostridium thermocellum

38000

-

SDS-PAGE

Hungateiclostridium thermocellum

Ni-nitrilotriacetic acid agarose colum

Hungateiclostridium thermocellum

21.3

-

beta-1,3-galactan as a substrate

Hungateiclostridium thermocellum

alpha-L-arabinofuranosidase-treated root arabinogalactan proteins from radish + H2O

relative activity: 9.3

677662

Hungateiclostridium thermocellum

?

-

?

beta-(1-3)-D-galactotriose + H2O

relative activity: 119%. Enzyme hydrolyzes beta-1,3-linked galacto-oligosaccharides but does not hydrolyze beta-1,4-linked and beta-1,6-linked galacto-oligosaccharide. Ct1,3Gal43A also can not hydrolyze the beta-1,3-galactosyl linkage of beta-1,3-galactosyl galactosaminide, beta-1,3-galactosyl glucosaminide, or beta-1,3-galactosyl L-arabinofuranoside

677662

Hungateiclostridium thermocellum

beta-(1-3)-D-galactobiose + D-galactose

-

?

beta-(1-3)-galactobiose + H2O

relative activity: 31%

677662

Hungateiclostridium thermocellum

D-galactose

-

?

beta-1,3-beta-1,6-galactan + H2O

relative activity: 3.1%

677662

Hungateiclostridium thermocellum

?

-

?

beta-1,3-galactan + H2O

relative activity: 100%. Enzyme catalyzes hydrolysis only of beta-1,3-linked galactosyl oligosaccharides and polysaccharides. Enzyme does not hydrolyze beta-1,4-linked galactose-containing polysaccharides, such as pectic galactan and alpha-L-arabinofuranosidase-treated pectic galactan from lupine, and also does not hydrolyze laminarin, CM-curdlan, or beta-1,3-xylan, suggesting that the enzyme acts only on a beta-1,3-linked galactan chain

677662

Hungateiclostridium thermocellum

oligo-beta-(1->3)-D-galactose + D-galactose

-

?

37

-

assay at

Hungateiclostridium thermocellum

50

-

Hungateiclostridium thermocellum

6

-

Hungateiclostridium thermocellum

3

10

-

Hungateiclostridium thermocellum

752571

Fujita

Degradative enzymes for type ...

Bifidobacterium longum subsp. Longum

Appl. Microbiol. Biotechnol.

103

1299-1310

2019

-

1

-

3

-

1

-

6

1

6

1

-

1

-

1

-

1

-

6

1

-

1

-

3

-

752397

Godoy

Crystal structure of a putati ...

Bifidobacterium bifidum, Bifidobacterium bifidum NCIMB 41171, Bifidobacterium bifidum S17

Acta Crystallogr. Sect. F

72

288-293

2016

-

1

-

1

-

5

-

1

-

3

2

-

1

-

1

-

1

-

1

-

3

2

-

1

-

731184

Fujita

Bifidobacterium longum subsp. ...

Bifidobacterium longum, Bifidobacterium longum JCM 1217

Appl. Environ. Microbiol.

80

4577-4584

2014

-

1

-

1

-

1

-

1

-

6

-

9

-

14

-

2

1

-

1

-

1

-

1

-

1

-

1

-

9

-

14

-

1

-

1

-

1

2

1

-

731202

Okawa

Identification of an exo-beta- ...

Fusarium oxysporum 12S, Fusarium oxysporum

Appl. Microbiol. Biotechnol.

97

9685-9694

2013

-

1

-

5

-

1

-

1

-

12

1

2

1

-

1

-

1

-

1

-

1

-

12

1

-

1

-

1

-

731581

Ling

An exo-beta-(1->3)-D-galactana ...

Streptomyces sp. 19(2012), Streptomyces sp.

Carbohydr. Res.

352

70-81

2012

-

1

-

3

4

-

6

-

1

-

1

-

12

1

4

1

-

2

-

1

-

2

-

3

4

-

2

-

2

-

12

2

-

2

-

2

-

1

2

-

732396

Jiang

Crystal structure of 1,3Gal43A ...

Hungateiclostridium thermocellum

J. Struct. Biol.

180

447-457

2012

-

1

2

-

2

-

4

-

1

-

1

2

3

1

-

1

-

1

-

1

2

-

2

-

1

-

1

2

1

-

1

-

2

-

713904

Sakamoto

Characterization of an exo-bet ...

Sphingomonas sp., Sphingomonas sp. 24T

Appl. Microbiol. Biotechnol.

90

1701-1710

2011

1

-

1

-

3

-

1

-

1

2

-

12

1

2

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

2

-

12

1

-

1

-

1

-

1

-

703061

Ling

Preparation of a new chromogen ...

Irpex lacteus

Carbohydr. Res.

344

1941-1946

2009

-

1

-

1

-

1

-

2

1

-

1

-

1

-

1

-

1

-

1

-

706965

Ishida

Crystallization of selenomethi ...

Phanerochaete chrysosporium

Acta Crystallogr. Sect. F

65

1274-1276

2009

-

1

-

2

-

1

-

1

3

-

1

-

1

-

1

-

1

-

707784

Kotake

Molecular cloning and expressi ...

Irpex lacteus, Irpex lacteus NBRC 5367

Biosci. Biotechnol. Biochem.

73

2303-2309

2009

-

1

-

1

-

2

-

1

-

1

-

4

1

2

1

-

1

-

1

-

1

-

1

-

1

-

4

1

-

1

-

677662

Ichinose

Characterization of an exo-bet ...

Hungateiclostridium thermocellum

Appl. Environ. Microbiol.

72

3515-3523

2006

-

1

-

1

-

4

-

1

-

1

-

5

-

2

-

1

-

1

-

1

-

1

-

1

-

5

-

2

-

1

-

678786

Ichinose

Characterization of an exo-bet ...

Streptomyces avermitilis, Streptomyces avermitilis NBRC 14893

Biosci. Biotechnol. Biochem.

70

2745-2750

2006

-

1

-

1

-

4

-

1

-

1

-

10

-

2

1

-

1

-

1

-

1

-

1

-

1

-

10

-

1

-

1

-

666634

Kotake

Molecular cloning of a b-galac ...

Raphanus sativus

Plant Physiol.

138

1563-1576

2005

-

1

-

4

3

-

2

-

4

-

1

-

1

-

10

1

-

1

3

1

-

1

-

4

-

3

-

2

-

1

-

1

-

10

1

-

1

3

1

-

171335

Tsumuraya

Purification of an exo-beta-(1 ...

Irpex lacteus

J. Biol. Chem.

265

7207-7215

1990

-

2

-

2

1

-

1

-

1

-

1

-

4

1

-

1

-

1

-

1

-

2

-

2

1

-

1

-

1

-

4

1

-

1

-

1

-

1

-

Characterization of an exo-beta -1,3-galactanase from Clostridium thermocellum

Data extracted from this reference: