Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli K12 TB1 and Rosetta ells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
L11D | the mutation increases enzyme activity to 148% | Homo sapiens |
L11D/L13D | the mutant almost entirely abolishes enzyme activity (4% activity) | Homo sapiens |
L11D/L13D/L14D | the mutant results in no detectable activity (less than 0.1% activity) | Homo sapiens |
additional information | deletion of the regulatory segment/MTS from full-length human PARG111 results in a complete loss of activity | Homo sapiens |
R10A | the mutation results in a significant increase in activity (144%) | Homo sapiens |
R2A/R3A/R6A/R10A | the mutant shows 113% enzyme activity | Homo sapiens |
R3A | the mutant shows 105% enzyme activity | Homo sapiens |
R6A | the mutation results in a significant increase in activity (248%) | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | full-length enzyme PARG111 | Homo sapiens | 5634 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00102 | - |
full length enzyme PARG111, pH and temperature not specified in the publication | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
poly(ADP-ribose) + H2O | - |
Homo sapiens | ADP-ribose oligomer + ADP-ribose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PARG | - |
Homo sapiens |
PARG111 | full-length enzyme | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | coordinate regulation of PARP-1 and -2 and PARG is required for cellular responses to genotoxic stress | Homo sapiens |