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Literature summary for 3.2.1.143 extracted from
- Purification and properties of an (ADP-ribose)n glycohydrolase from guinea pig liver nuclei (1986), J. Biol. Chem., 261, 965-969.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 10 mM, 3fold stimulation | Cavia porcellus |  |
| dithiothreitol | 10 mM, 3fold stimulation | Cavia porcellus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (17-chloro-3b-hydroxy-androsta-5,16-diene)-succinyl-glutathione | - |
Cavia porcellus | |
| adenosine 3':5'-cyclic monophosphate | - |
Cavia porcellus | |
| ADP-ribose | - |
Cavia porcellus | |
| CaCl2 | 1.5 mM, 50% inhibition. 96% inhibition at 10 mM | Cavia porcellus | |
| DNA | denatured; no inhibition by native DNA | Cavia porcellus | |
| histone | - |
Cavia porcellus | |
| KCl | 200 mM or more | Cavia porcellus | |
| NaCl | 200 mM or more | Cavia porcellus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0023 | - |
(ADP-ribose)n | - |
Cavia porcellus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | 50 mM, 1.4-1.5fold stimulation | Cavia porcellus | |
| NaCl | 50 mM, 1.4-1.5fold stimulation | Cavia porcellus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 72000 | - |
gel filtration | Cavia porcellus |
| 75500 | - |
1 * 75500, SDS-PAGE | Cavia porcellus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cavia porcellus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Cavia porcellus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Cavia porcellus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 32.5 | - |
- |
Cavia porcellus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| poly(ADP-ribose)n + H2O | - |
Cavia porcellus | ADP-ribose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 75500, SDS-PAGE | Cavia porcellus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 7 | - |
Cavia porcellus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | about 55% of maximal activity at pH 6.0 and 8.0 | Cavia porcellus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.5 | - |
CaCl2 | - |
Cavia porcellus | |
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