Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Exiguobacterium acetylicum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 5 mM, 51% residual activity | Exiguobacterium acetylicum | |
Cu2+ | 5 mM, 67% residual activity | Exiguobacterium acetylicum | |
EDTA | 5 mM, 72% residual activity | Exiguobacterium acetylicum | |
Mg2+ | 5 mM, 90% residual activity | Exiguobacterium acetylicum | |
SDS | 5 mM, 46% residual activity | Exiguobacterium acetylicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
pullulan | Km value 0.12 mg/ml, pH 6.0, 50°C | Exiguobacterium acetylicum | |
additional information | - |
soluble starch | Km value 0.69 mg/ml,pH 6.0, 50°C | Exiguobacterium acetylicum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 5 mM, 198% of initial activity | Exiguobacterium acetylicum | |
Fe2+ | 5 mM, 121% of initial activity | Exiguobacterium acetylicum | |
Mn2+ | 5 mM, 136% of initial activity | Exiguobacterium acetylicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
95800 | - |
- |
Exiguobacterium acetylicum |
100000 | - |
PAGE | Exiguobacterium acetylicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Exiguobacterium acetylicum | I3WU34 | - |
- |
Exiguobacterium acetylicum YH5 | I3WU34 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | 6.0% of the activity with pullulan | Exiguobacterium acetylicum | maltotriose + maltose + maltooligosaccharides | - |
? | |
beta-limited dextrin + H2O | 19.7% of the activity with pullulan | Exiguobacterium acetylicum | maltotriose + maltose + maltooligosaccharides | - |
? | |
glycogen + H2O | 6.1% of the activity with pullulan | Exiguobacterium acetylicum | maltotriose + maltose + maltooligosaccharides | - |
? | |
additional information | no substrates: maltotriose, maltose. the enzyme is strictly restricted to hydrolysis of 1->6-linked glucosidic linkages. It also acts as type I pullulanase, EC 3.2.1.41 | Exiguobacterium acetylicum | ? | - |
? | |
pullulan + H2O | - |
Exiguobacterium acetylicum | maltotriose + ? | complete conversion to maltotriose | ? | |
soluble starch + H2O | 12.8% of the activity with pullulan | Exiguobacterium acetylicum | maltotriose + maltose + maltooligosaccharides | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 100000, SDS-PAGE, 1 * 95800, calculated | Exiguobacterium acetylicum |
Synonyms | Comment | Organism |
---|---|---|
Pul3YH5 | - |
Exiguobacterium acetylicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Exiguobacterium acetylicum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
pH 5-8, 10 min, more than 70% of maximum activity | Exiguobacterium acetylicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Exiguobacterium acetylicum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | 30 min, more than 93% residual activity | Exiguobacterium acetylicum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Exiguobacterium acetylicum | calculated | - |
6.5 |