Literature summary for 3.2.1.142 extracted from

Moller, M.S.; Windahl, M.S.; Sim, L.; Bojstrup, M.; Abou Hachem, M.; Hindsgaul, O.; Palcic, M.; Svensson, B.; Henriksen, A.
Oligosaccharide and substrate binding in the starch debranching enzyme barley limit dextrinase (2015), J. Mol. Biol., 427, 1263-1277.

Search for more literature for 3.2.1.142

Crystallization (Commentary)

Crystallization (Comment)	Organism
structures of barley limit dextrinase and active-site mutants in complex with natural substrates, products and substrate analogues. Avoidance of alpha-1,4-hydrolytic activity and strong preference for alpha-1,6-hydrolytic activity seems to be based on the strong interaction to Trp512 and Phe553 on each flank of the catalytic cleft, which will keep non-branched polysaccharides out of the reach of the catalytic nucleophile and acid-base catalyst	Hordeum vulgare

Crystallization (Comment)

Organism

structures of barley limit dextrinase and active-site mutants in complex with natural substrates, products and substrate analogues. Avoidance of alpha-1,4-hydrolytic activity and strong preference for alpha-1,6-hydrolytic activity seems to be based on the strong interaction to Trp512 and Phe553 on each flank of the catalytic cleft, which will keep non-branched polysaccharides out of the reach of the catalytic nucleophile and acid-base catalyst

Hordeum vulgare

Protein Variants

Protein Variants	Comment	Organism
E510A	0.0004% residual activity with pullulan	Hordeum vulgare
M440G	2.6fold decrease in catalytic efficiency	Hordeum vulgare

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	pullulan	Km value of wild-type 0.16 mg/ml, mutant M440G 0.15 mg/ml, respectively, 37°C, pH not specified in the publication	Hordeum vulgare
additional information	-	amylopectin	Km value of wild-type 6.9 mg/ml, mutant M440G 4.4 mg/ml, respectively, 37°C, pH not specified in the publication	Hordeum vulgare

Organism

Organism	UniProt	Comment	Textmining
Hordeum vulgare	O48541	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
amylopectin + H2O	-	Hordeum vulgare	?	-	?
pullulan + H2O	-	Hordeum vulgare	?	-	?

Synonyms

Synonyms	Comment	Organism
HvLD99	-	Hordeum vulgare

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
3.9	-	amylopectin	mutant M440G, 37°C, pH not specified in the publication	Hordeum vulgare
15.6	-	amylopectin	wild-type, 37°C, pH not specified in the publication	Hordeum vulgare
72	-	pullulan	mutant M440G, 37°C, pH not specified in the publication	Hordeum vulgare
78	-	pullulan	wild-type, 37°C, pH not specified in the publication	Hordeum vulgare

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
additional information	-	amylopectin	kcat/Km value of wild-type 2.3 ml/mg/s, mutant M440G 0.9 ml/mg/s, respectively, 37°C, pH not specified in the publication	Hordeum vulgare
additional information	-	pullulan	kcat/Km value of wild-type 488 ml/mg/s, mutant M440G 480 ml/mg/s, respectively, 37°C, pH not specified in the publication	Hordeum vulgare