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Literature summary for 3.2.1.142 extracted from

  • Kahar, U.M.; Ng, C.L.; Chan, K.G.; Goh, K.M.
    Characterization of a type I pullulanase from Anoxybacillus sp. SK3-4 reveals an unusual substrate hydrolysis (2016), Appl. Microbiol. Biotechnol., 100, 6291-6307.
    View publication on PubMed
Search for more literature for 3.2.1.142

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 2 mM, 143% of initial activtiy Anoxybacillus sp.
Tween-20 1% v/v, 143% of initial activtiy Anoxybacillus sp.

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Anoxybacillus sp.

Protein Variants

Protein Variants Comment Organism
additional information amino acids 362-370 of Ask are replaced with the corresponding sequence of type I pullulanase Pul-LM14-2 from Anoxybacillus sp. LM14-2. Unlike wild-type, the mutant enzyme forms reducing sugars on digesting starch Anoxybacillus sp.

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 2 mM, 54% of initial activtiy Anoxybacillus sp.
Co2+ 2 mM, 15% of initial activtiy Anoxybacillus sp.
Cu2+ 2 mM, 8% of initial activtiy Anoxybacillus sp.
EDTA 2 mM, 68% of initial activtiy Anoxybacillus sp.
Fe2+ 2 mM, 6% of initial activtiy Anoxybacillus sp.
Mn2+ 2 mM, 40% of initial activtiy Anoxybacillus sp.
NH4+ 2 mM, 49% of initial activtiy Anoxybacillus sp.
Ni2+ 2 mM, 47% of initial activtiy Anoxybacillus sp.
Sr2+ 2 mM, 73% of initial activtiy Anoxybacillus sp.
Triton X-100 1% v/v, 72% of initial activtiy Anoxybacillus sp.
Urea 2 mM, 57% of initial activtiy Anoxybacillus sp.
Zn2+ 2 mM, 22% of initial activtiy Anoxybacillus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
K+ 2 mM, 132% of initial activtiy Anoxybacillus sp.
Mg2+ 2 mM, 122% of initial activtiy Anoxybacillus sp.
Na+ 2 mM, 128% of initial activtiy Anoxybacillus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
 x * 80000, SDS-PAGE Anoxybacillus sp.

Organic Solvent Stability

Organic Solvent Comment Organism
DMSO 2 mM, 59% of initial activtiy Anoxybacillus sp.

Organism

Organism UniProt Comment Textmining
Anoxybacillus sp. EPZ37738
-
-
Anoxybacillus sp. SK3-4 EPZ37738
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
limit dextrin + H2O
-
 Anoxybacillus sp. maltotriose + maltotetraose + maltopentaose
-
 ?
limit dextrin + H2O
-
 Anoxybacillus sp. SK3-4 maltotriose + maltotetraose + maltopentaose
-
 ?
additional information the enzyme preferably debranches long branches at alpha-1,6 glycosidic bonds of starch, producing amylose, linear or branched oligosaccharides, but is nonreactive against short branches. Main reaction is as a type I pullulanase, EC 3.2.1.41 Anoxybacillus sp. ?
-
 ?
additional information the enzyme preferably debranches long branches at alpha-1,6 glycosidic bonds of starch, producing amylose, linear or branched oligosaccharides, but is nonreactive against short branches. Main reaction is as a type I pullulanase, EC 3.2.1.41 Anoxybacillus sp. SK3-4 ?
-
 ?
pullulan + H2O
-
 Anoxybacillus sp. maltotriose + ?
-
 ?
pullulan + H2O
-
 Anoxybacillus sp. SK3-4 maltotriose + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 80000, SDS-PAGE Anoxybacillus sp.

Synonyms

Synonyms Comment Organism
Ask
-
 Anoxybacillus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Anoxybacillus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 60 20 min, stable Anoxybacillus sp.
60
-
 9 h, 50% residual activity Anoxybacillus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Anoxybacillus sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 6
-
 Anoxybacillus sp.