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Literature summary for 3.2.1.142 extracted from

  • Boejstrup, M.; Christensen, C.E.; Windahl, M.S.; Henriksen, A.; Hindsgaul, O.
    A chromogenic assay for limit dextrinase and pullulanase activity (2014), Anal. Biochem., 449, 45-51.
    View publication on PubMed
Search for more literature for 3.2.1.142

Activating Compound

Activating Compound Comment Organism Structure
DTT
-
 Hordeum vulgare

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression in Pichia pastoris and Escherichia coli Hordeum vulgare

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics Hordeum vulgare
0.135
-
 2-chloro-4-nitrophenyl maltotriosyl-alpha(1->6)-maltotriosyl-beta-D-glucose pH 5.3, 45°C, barley malt extract Hordeum vulgare
0.154
-
 2-chloro-4-nitrophenyl maltotriosyl-alpha(1->6)-maltotriosyl-beta-D-glucose pH 5.3, 45°C, recombinant enzyme Hordeum vulgare

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Hordeum vulgare

Organism

Organism UniProt Comment Textmining
Hordeum vulgare
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-chloro-4-nitrophenyl maltotriosyl-alpha(1->6)-maltotriosyl-beta-D-glucose + H2O
-
 Hordeum vulgare 2-chloro-4-nitrophenyl maltotriose + maltotriosyl-beta-D-glucose
-
 ?
additional information no activity with glycogen Hordeum vulgare ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 assay at Hordeum vulgare

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3
-
 assay at Hordeum vulgare