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Literature summary for 3.2.1.142 extracted from

  • Gordon, R.W.; Manners, D.J.; Stark, J.R.
    The limit dextrinase of the broad bean (Vicia faba L.) (1975), Carbohydr. Res., 42, 125-134.
No PubMed abstract available
Search for more literature for 3.2.1.142

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.16
-
 6-alpha-maltotriosylmaltotetraose
-
 Vicia faba
0.6
-
 amylopectin beta-limit dextrin
-
 Vicia faba

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
 gel filtration Vicia faba

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
polysaccharide + H2O Vicia faba
-
 oligosaccharides
-
 ?

Organism

Organism UniProt Comment Textmining
Vicia faba
-
 broad bean
-

Purification (Commentary)

Purification (Comment) Organism
acetone precipitation, DEAE-cellulose, Sephadex G-150 Vicia faba

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.04
-
-
 Vicia faba

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-alpha-maltotriosylmaltotetraose + H2O
-
 Vicia faba ?
-
 ?
amylopectin beta-limit dextrin + H2O
-
 Vicia faba maltotriose + maltose + maltooligosaccharides
-
 ?
polysaccharide + H2O
-
 Vicia faba oligosaccharides
-
 ?
polysaccharide + H2O oligosaccharides containing maltosyl or larger side chains are hydrolysed as well as amylopectin and amylopectin beta-limit dextrin, oligo- and poly-saccharides containing single D-glucosyl groups attached by an alpha-(1,6) linkage to the rest of the molecule are not hydrolysed, glycogen is not a substrate Vicia faba oligosaccharides
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4 6.8 pullulan and amylopectin as substrates, maximum activity occurred over a relatively broad range Vicia faba