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Literature summary for 3.2.1.142 extracted from
- The limit dextrinase from ungerminated oats (Avena sativa L.) and ungerminated rice (Oryza sativa L.) (1975), Carbohydr. Res., 39, 283-293.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 6,3-alpha-D-glucosylmaltotriose | - |
Oryza sativa |  |
| 6,3-alpha-D-maltosylmaltotetraose | - |
Oryza sativa | |
| 6,3-alpha-D-maltosylmaltotriose | - |
Oryza sativa | |
| 6,3-alpha-D-maltotriosylmaltotetraose | - |
Oryza sativa | |
| 6,3-alpha-D-maltotriosylmaltotriose | - |
Oryza sativa | |
| maltose | - |
Oryza sativa | |
| oligomaltose | - |
Oryza sativa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
6-alpha-maltotriosylmaltotetraose | - |
Avena sativa | |
| 0.2 | - |
6-alpha-maltosylmaltotetraose | - |
Avena sativa | |
| 0.2 | - |
6-alpha-maltosylmaltotriose | - |
Avena sativa | |
| 0.2 | - |
6-alpha-maltotriosylmaltotriose | - |
Avena sativa | |
| 0.6 | - |
6-alpha-maltotriosylmaltotetraose | - |
Oryza sativa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 80000 | - |
preliminary studies, gel filtration | Avena sativa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| polysaccharide + H2O | Avena sativa | - |
oligosaccharides | - |
? | |
| polysaccharide + H2O | Oryza sativa | - |
oligosaccharides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Avena sativa | - |
ungerminated oats | - |
| Oryza sativa | - |
ungerminated rice | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| acetone precipitation, DEAE-cellulose, Sephadex G-150 | Avena sativa |
| acetone precipitation, DEAE-cellulose, Sephadex G-150 | Oryza sativa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.6 | - |
- |
Oryza sativa |
| 0.8 | - |
- |
Avena sativa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-alpha-maltosylmaltotetraose + H2O | - |
Avena sativa | ? | - |
? | |
| 6-alpha-maltosylmaltotriose + H2O | - |
Avena sativa | ? | - |
? | |
| 6-alpha-maltotriosylmaltotetraose + H2O | - |
Avena sativa | ? | - |
? | |
| 6-alpha-maltotriosylmaltotetraose + H2O | - |
Oryza sativa | ? | - |
? | |
| polysaccharide + H2O | - |
Avena sativa | oligosaccharides | - |
? | |
| polysaccharide + H2O | - |
Oryza sativa | oligosaccharides | - |
? | |
| polysaccharide + H2O | oligosaccharides containing maltosyl or larger side chains are hydrolysed as well as amylopectin and amylopectin beta-limit dextrin, oligo- and poly-saccharides containing single D-glucosyl groups attached by an alpha-(1,6) linkage to the rest of the molecule are not hydrolysed, glycogen is not a substrate | Avena sativa | oligosaccharides | - |
? | |
| polysaccharide + H2O | oligosaccharides containing maltosyl or larger side chains are hydrolysed as well as amylopectin and amylopectin beta-limit dextrin, oligo- and poly-saccharides containing single D-glucosyl groups attached by an alpha-(1,6) linkage to the rest of the molecule are not hydrolysed, glycogen is not a substrate | Oryza sativa | oligosaccharides | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Avena sativa |
| 5 | - |
- |
Oryza sativa |
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