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Literature summary for 3.2.1.141 extracted from

  • Zhou, Y.; Xie, G.; Chang, L.; Wang, Y.; Gao, R.
    Identification of an archaeal maltooligosyltrehalose trehalohydrolase encoded by an interrupted gene (2017), Extremophiles, 21, 537-549 .
    View publication on PubMed
Search for more literature for 3.2.1.141

Activating Compound

Activating Compound Comment Organism Structure
additional information poor effect by EDTA Sulfurisphaera tokodaii

Cloned(Commentary)

Cloned (Comment) Organism
gene ST0926, DNA and amino acid sequence determination and analysis, sequence comparisons, a specific deletion mutant is expressed as His6-tagged protein in Escherichia coli as soluble, active acidic and thermophilic enzyme. ORFs ST0926/ST0927 in the Archaeon Sulfurisphaera tokodaii strain 7 cannot be expressed in vivo, indicating they might exist as nonfunctional pseudogenes Sulfurisphaera tokodaii
gene ST0927, DNA and amino acid sequence determination and analysis, sequence comparisons, a specific deletion mutant is expressed as His6-tagged protein in Escherichia coli as soluble, active acidic and thermophilic enzyme. ORFs ST0926/ST0927 in the Archaeon Sulfurisphaera tokodaii strain 7 cannot be expressed in vivo, indicating they might exist as nonfunctional pseudogenes Sulfurisphaera tokodaii

Protein Variants

Protein Variants Comment Organism
additional information generation of a specific deletion mutation, designed on the basis of sequence alignments of the adjacent ORFs and the published Sulfolobus sp. MTHases, allowing soluble expression in Escherichia coli as active acidic and thermophilic enzyme Sulfurisphaera tokodaii

Inhibitors

Inhibitors Comment Organism Structure
Fe3+
-
 Sulfurisphaera tokodaii

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ activates slightly Sulfurisphaera tokodaii
additional information no or poor effects by Na+, K+, Ca2+, Mg2+, Cu2+, Zn2+, and Ni2+ Sulfurisphaera tokodaii

Organism

Organism UniProt Comment Textmining
Sulfurisphaera tokodaii Q973H4 i.e. Sulfolobus tokodaii
-
Sulfurisphaera tokodaii Q973H5 i.e. Sulfolobus tokodaii
-
Sulfurisphaera tokodaii 7 Q973H4 i.e. Sulfolobus tokodaii
-
Sulfurisphaera tokodaii 7 Q973H5 i.e. Sulfolobus tokodaii
-
Sulfurisphaera tokodaii DSM 16993 Q973H4 i.e. Sulfolobus tokodaii
-
Sulfurisphaera tokodaii DSM 16993 Q973H5 i.e. Sulfolobus tokodaii
-
Sulfurisphaera tokodaii JCM 10545 Q973H4 i.e. Sulfolobus tokodaii
-
Sulfurisphaera tokodaii JCM 10545 Q973H5 i.e. Sulfolobus tokodaii
-
Sulfurisphaera tokodaii NBRC 100140 Q973H4 i.e. Sulfolobus tokodaii
-
Sulfurisphaera tokodaii NBRC 100140 Q973H5 i.e. Sulfolobus tokodaii
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme mutant from Escherichia coli by nickel affinity chromatography, followed by anion exchange chromatography, and multiple dialysis steps Sulfurisphaera tokodaii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the recombinant mutant enzyme is active against starch in combination with enzyme MTSase, EC 5.4.99.15, that produces trehalose from starch Sulfurisphaera tokodaii ?
-
 ?
additional information the recombinant mutant enzyme is active against starch in combination with enzyme MTSase, EC 5.4.99.15, that produces trehalose from starch Sulfurisphaera tokodaii 7 ?
-
 ?
additional information the recombinant mutant enzyme is active against starch in combination with enzyme MTSase, EC 5.4.99.15, that produces trehalose from starch Sulfurisphaera tokodaii DSM 16993 ?
-
 ?
additional information the recombinant mutant enzyme is active against starch in combination with enzyme MTSase, EC 5.4.99.15, that produces trehalose from starch Sulfurisphaera tokodaii JCM 10545 ?
-
 ?
additional information the recombinant mutant enzyme is active against starch in combination with enzyme MTSase, EC 5.4.99.15, that produces trehalose from starch Sulfurisphaera tokodaii NBRC 100140 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 65000, about, putative mature StMTHase protein lacking the N-terminal Trx-tag and His6-tag, sequence calculation Sulfurisphaera tokodaii

Synonyms

Synonyms Comment Organism
maltooligosyltrehalose trehalohydrolase
-
 Sulfurisphaera tokodaii
ST0926
-
 Sulfurisphaera tokodaii
ST0927
-
 Sulfurisphaera tokodaii
StMTHase
-
 Sulfurisphaera tokodaii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
 recombinant enzyme mutant Sulfurisphaera tokodaii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60 80 over 90% of maximal activity within this range, recombinant enzyme mutant Sulfurisphaera tokodaii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 recombinant enzyme mutant Sulfurisphaera tokodaii

pH Range

pH Minimum pH Maximum Comment Organism
3.5 6 50% of maximal activity within this range, recombinant enzyme mutant Sulfurisphaera tokodaii

General Information

General Information Comment Organism
additional information prediction analysis of putative structural domains of ST0926/ ST0927 and modeling using crystal structure, PDB ID 1EH9, of Sulfurisphaera tokodaii KM1 MTHase as template Sulfurisphaera tokodaii