Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method at 4°C. The crystal structure of the E283Q mutant enzyme in complex with the substrate, maltotriosyltrehalose is determined to 2.6 A resolution. The structure with maltotriosyltrehalose indicates that the enzyme has at least five substrate binding subsites and that Glu283 is the catalytic acid, and Asp252 is the nucleophile that attacks the C1 carbon in the glycosidic linkage of maltotriosyltrehalose | Saccharolobus solfataricus |
Protein Variants | Comment | Organism |
---|---|---|
D252E | mutant shows 0.03% of enzymatic activity compared to the wild-type | Saccharolobus solfataricus |
D252S | mutant shows 0.6% of enzymatic activity compared to the wild-type | Saccharolobus solfataricus |
E283Q | mutant shows 0.04% of enzymatic activity compared to the wild-type | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | Q55088 | - |
- |
Saccharolobus solfataricus KM1 | Q55088 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the crystal structure reveals a scheme for substrate recognition that involves two unique interactions: stacking of Tyr325 with the terminal glucose ring of the trehalose moiety and perpendicularly placement of Trp215 to the pyranose rings at the subsites -1 and +1 glucose | Saccharolobus solfataricus | ? | - |
? | |
additional information | the crystal structure reveals a scheme for substrate recognition that involves two unique interactions: stacking of Tyr325 with the terminal glucose ring of the trehalose moiety and perpendicularly placement of Trp215 to the pyranose rings at the subsites -1 and +1 glucose | Saccharolobus solfataricus KM1 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glycosyltrehalose trehalohydrolase | - |
Saccharolobus solfataricus |
GTHase | - |
Saccharolobus solfataricus |