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Literature summary for 3.2.1.141 extracted from

  • Shuangxiu, W.; He, L.; Shen, R.; Zhang, X.; Wang, Q.
    Molecular cloning of maltooligosyltrehalose trehalohydrolase gene from Nostoc flagelliforme and trehalose-related response to stresses (2011), J. Microbiol. Biotechnol., 21, 830-837.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) pLysS cells Nostoc flagelliforme

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
x * 70000, deduced from amino acid sequence Nostoc flagelliforme
75000
-
x * 75000, including the hexahistidine domain from the pET22bZ vector, SDS-PAGE Nostoc flagelliforme

Organism

Organism UniProt Comment Textmining
Nostoc flagelliforme E1ANS4
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-NTA-agarose column chromatography, gel filtration Nostoc flagelliforme

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltooligosyltrehalose + H2O
-
Nostoc flagelliforme maltooligosaccharide + alpha,alpha'-trehalose
-
?

Subunits

Subunits Comment Organism
? x * 70000, deduced from amino acid sequence Nostoc flagelliforme
? x * 75000, including the hexahistidine domain from the pET22bZ vector, SDS-PAGE Nostoc flagelliforme

Synonyms

Synonyms Comment Organism
maltooligosyltrehalose trehalohydrolase
-
Nostoc flagelliforme
MTH
-
Nostoc flagelliforme

Expression

Organism Comment Expression
Nostoc flagelliforme the expressions of maltooligosyltrehalose trehalohydrolase in the filaments of Nostoc flagelliforme are upregulated significantly under dehydration stress (exposition to air for 24 h), NaCl stress (exposition to 24-175 mM NaCl for 4 h), and high temperature-drought stress (exposition to air at various 4°C, 10°C, 25°C, 37°C, and 42°C, respectively, for 4 h) up