Literature summary for 3.2.1.141 extracted from

  • Seo, J.S.; An, J.H.; Baik, M.Y.; Park, C.S.; Cheong, J.J.; Moon, T.W.; Park, K.H.; Choi, Y.D.; Kim, C.H.
    Molecular cloning and characterization of trehalose biosynthesis genes from hyperthermophilic archaebacterium Metallosphaera hakonesis (2007), J. Microbiol. Biotechnol., 17, 123-129.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information addition of 4-alpha-glucanotransferase increases the yield of trehalose production from maltooligosaccharide substrates supplying longer maltooligosaccharides that may be better substrates for the sequential reaction catalyzed by MTS and MTH Metallosphaera hakonensis
IPTG transcription induced by 1 mM Metallosphaera hakonensis

Cloned(Commentary)

Cloned (Comment) Organism
DNA construct pRBMhMTH expressed in Escherichia coli BL21(DE3)pLysS Metallosphaera hakonensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63700
-
sequence analysis Metallosphaera hakonensis

Organism

Organism UniProt Comment Textmining
Metallosphaera hakonensis
-
JCM 8857
-
Metallosphaera hakonensis JCM 8857
-
JCM 8857
-

Purification (Commentary)

Purification (Comment) Organism
by Ni2+-NTA-agarose affinity chromatography Metallosphaera hakonensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltotriosyltrehalose + H2O
-
Metallosphaera hakonensis maltotriose + alpha,alpha'-trehalose
-
?
maltotriosyltrehalose + H2O
-
Metallosphaera hakonensis JCM 8857 maltotriose + alpha,alpha'-trehalose
-
?
4'-O-(1,4-alpha-D-glucosyl)n-alpha,alpha'-trehalose + H2O yield of trehalose production is relatively low but may be increased in the presence of alpha-amylase Metallosphaera hakonensis (1,4-alpha-D-glucosyl)n + alpha,alpha'-trehalose
-
?
4'-O-(1,4-alpha-D-glucosyl)n-alpha,alpha'-trehalose + H2O yield of trehalose production is relatively low but may be increased in the presence of alpha-amylase Metallosphaera hakonensis JCM 8857 (1,4-alpha-D-glucosyl)n + alpha,alpha'-trehalose
-
?

Synonyms

Synonyms Comment Organism
Maltooligosyl trehalose trehalohydrolase
-
Metallosphaera hakonensis
MTH
-
Metallosphaera hakonensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Metallosphaera hakonensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
retainis 80% of the activity for 48 h, activity is gradually abolished at above 85°C Metallosphaera hakonensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Metallosphaera hakonensis