Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | EDTA does not activate. Expression levels of both wild-type and His-tagged MTHases are higher in the absence of IPTG than in the presence of 0.5 mM IPTG induction | Saccharolobus solfataricus |
Cloned (Comment) | Organism |
---|---|
pET-15b-deltaH-treZ vector expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | inhibits at 4 mM | Saccharolobus solfataricus | |
Hg2+ | 0.4 and 4 mM strongly inhibit | Saccharolobus solfataricus | |
additional information | EDTA, Na+, K+, Mg2+, Ca2+, and Zn2+ do not inhibit | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.63 | - |
maltoheptaose | - |
Saccharolobus solfataricus | |
5.63 | - |
maltohexaose | - |
Saccharolobus solfataricus | |
5.66 | - |
maltotetraosyl trehalose | - |
Saccharolobus solfataricus | |
5.86 | - |
maltopentaose | - |
Saccharolobus solfataricus | |
5.89 | - |
maltopentaosyl trehalose | - |
Saccharolobus solfataricus | |
7.22 | - |
maltotriosyl trehalose | - |
Saccharolobus solfataricus | |
10.9 | - |
maltotetraose | - |
Saccharolobus solfataricus | |
11.1 | - |
maltosyl trehalose | - |
Saccharolobus solfataricus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | Na+, K+, Mg2+, Ca2+, and Zn2+ do not activate | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
64000 | - |
2 * 64000, SDS-PAGE, 2 * 64240, electrospray ionization mass spectrometry, wild-type enzyme | Saccharolobus solfataricus |
64240 | - |
2 * 64000, SDS-PAGE, 2 * 64240, electrospray ionization mass spectrometry, wild-type enzyme | Saccharolobus solfataricus |
66000 | - |
1 * 66000, SDS-PAGE, His-tagged enzyme | Saccharolobus solfataricus |
68700 | - |
gel filtration, His-tagged enzyme | Saccharolobus solfataricus |
128800 | - |
gel filtration, wild-type enzyme | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | P95867 | ATCC 35092 | - |
Purification (Comment) | Organism |
---|---|
wild-type enzyme purified by heat treatment, nucleic acid precipitation, and ion-exchange chromatography, 12fold. His-tagged MTHase partially purified from the cell-free extract directly by metal chelating chromatography, 14.7fold | Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8 | 12 | wild-type, 12fold purified | Saccharolobus solfataricus |
21.1 | - |
His-tagged enzyme, crude extract | Saccharolobus solfataricus |
67.5 | - |
wild-type, crude extract | Saccharolobus solfataricus |
310 | - |
His-tagged enzyme, 14.7fold purified | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltoheptaose + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
maltohexaose + H2O | for glucose formation, maltohexaose exhibits the highest formation rate | Saccharolobus solfataricus | ? | - |
? | |
maltopentaose + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
maltopentaosyltrehalose + H2O | trehalose formation rate increases as the degree of polymerization value of the substrate increases. maltopentaosyl trehalose is the most preferred substrate for trehalose formation | Saccharolobus solfataricus | maltopentaose + alpha,alpha'-trehalose | - |
? | |
maltosyltrehalose + H2O | - |
Saccharolobus solfataricus | maltose + alpha,alpha'-trehalose | - |
? | |
maltotetraose + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
maltotetraosyltrehalose + H2O | - |
Saccharolobus solfataricus | maltotetraose + alpha,alpha'-trehalose | - |
? | |
maltotriosyltrehalose + H2O | - |
Saccharolobus solfataricus | maltotriose + alpha,alpha'-trehalose | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 64000, SDS-PAGE, 2 * 64240, electrospray ionization mass spectrometry, wild-type enzyme | Saccharolobus solfataricus |
monomer | 1 * 66000, SDS-PAGE, His-tagged enzyme | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
Maltooligosyl trehalose trehalohydrolase | - |
Saccharolobus solfataricus |
MTHase | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
- |
Saccharolobus solfataricus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 85 | - |
Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
retains all of its activity for 2 h | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
maltoheptaose | - |
Saccharolobus solfataricus | |
5.49 | - |
maltotetraose | - |
Saccharolobus solfataricus | |
6.01 | - |
maltoheptaose | - |
Saccharolobus solfataricus | |
17.7 | - |
maltopentaose | - |
Saccharolobus solfataricus | |
18.2 | - |
maltohexaose | - |
Saccharolobus solfataricus | |
193 | - |
maltosyl trehalose | - |
Saccharolobus solfataricus | |
1030 | - |
maltotriosyl trehalose | - |
Saccharolobus solfataricus | |
1190 | - |
maltotetraosyl trehalose | - |
Saccharolobus solfataricus | |
1230 | - |
maltopentaosyl trehalose | - |
Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Saccharolobus solfataricus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 11 | - |
Saccharolobus solfataricus |