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Literature summary for 3.2.1.141 extracted from
- Purification and characterization of thermostable maltooligosyl trehalose trehalohydrolase from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius (1996), Biosci. Biotechnol. Biochem., 60, 267-270.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Sulfolobus acidocaldarius |  |
| Fe2+ | slight | Sulfolobus acidocaldarius | |
| Hg2+ | - |
Sulfolobus acidocaldarius | |
| Pb2+ | slight | Sulfolobus acidocaldarius | |
| Zn2+ | slight | Sulfolobus acidocaldarius | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.7 | - |
maltotriosyl trehalose | - |
Sulfolobus acidocaldarius | |
| 3.7 | - |
maltotetraosyl trehalose | - |
Sulfolobus acidocaldarius | |
| 4.9 | - |
maltopentaosyl trehalose | - |
Sulfolobus acidocaldarius | |
| 16.7 | - |
maltosyl trehalose | - |
Sulfolobus acidocaldarius | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 59000 | - |
x * 59000, SDS-PAGE | Sulfolobus acidocaldarius |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sulfolobus acidocaldarius | - |
ATCC 33909 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sulfolobus acidocaldarius |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 730 | - |
- |
Sulfolobus acidocaldarius |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glucosyl trehalose + H2O | - |
Sulfolobus acidocaldarius | glucose + trehalose | - |
? | |
| maltopentaosyltrehalose + H2O | - |
Sulfolobus acidocaldarius | maltopentaose + alpha,alpha'-trehalose | - |
? | |
| maltosyltrehalose + H2O | - |
Sulfolobus acidocaldarius | maltose + alpha,alpha'-trehalose | - |
? | |
| maltotetraosyltrehalose + H2O | - |
Sulfolobus acidocaldarius | maltotetraose + alpha,alpha'-trehalose | - |
? | |
| maltotriosyltrehalose + H2O | - |
Sulfolobus acidocaldarius | maltotriose + alpha,alpha'-trehalose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 59000, SDS-PAGE | Sulfolobus acidocaldarius |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
- |
Sulfolobus acidocaldarius |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 90 | 40°C: about 45% of maximal activity, 90°C: about 65% of maximal activity | Sulfolobus acidocaldarius |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
60 min, stable below | Sulfolobus acidocaldarius |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 6 | - |
Sulfolobus acidocaldarius |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7 | pH 5: about 80% of maximal activity, pH 7: about 65% of maximal activity | Sulfolobus acidocaldarius |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 6 | 4°C, 24 h, stable | Sulfolobus acidocaldarius |
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