Literature summary for 3.2.1.141 extracted from

  • Nakada, T.; Ikegami, S.; Chaen, H.; Kubota, M.; Fukuda, S.; Sugimoto, T.; Kurimoto, M.; Tsujisaka, Y.
    Purification and characterization of thermostable maltooligosyl trehalose trehalohydrolase from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius (1996), Biosci. Biotechnol. Biochem., 60, 267-270.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
Sulfolobus acidocaldarius
Fe2+ slight Sulfolobus acidocaldarius
Hg2+
-
Sulfolobus acidocaldarius
Pb2+ slight Sulfolobus acidocaldarius
Zn2+ slight Sulfolobus acidocaldarius

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.7
-
maltotetraosyl trehalose
-
Sulfolobus acidocaldarius
2.7
-
maltotriosyl trehalose
-
Sulfolobus acidocaldarius
4.9
-
maltopentaosyl trehalose
-
Sulfolobus acidocaldarius
16.7
-
maltosyl trehalose
-
Sulfolobus acidocaldarius

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59000
-
x * 59000, SDS-PAGE Sulfolobus acidocaldarius

Organism

Organism UniProt Comment Textmining
Sulfolobus acidocaldarius
-
ATCC 33909
-

Purification (Commentary)

Purification (Comment) Organism
-
Sulfolobus acidocaldarius

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
730
-
-
Sulfolobus acidocaldarius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glucosyl trehalose + H2O
-
Sulfolobus acidocaldarius glucose + trehalose
-
?
maltosyltrehalose + H2O
-
Sulfolobus acidocaldarius maltose + alpha,alpha'-trehalose
-
?
maltotriosyltrehalose + H2O
-
Sulfolobus acidocaldarius maltotriose + alpha,alpha'-trehalose
-
?
maltotetraosyltrehalose + H2O
-
Sulfolobus acidocaldarius maltotetraose + alpha,alpha'-trehalose
-
?
maltopentaosyltrehalose + H2O
-
Sulfolobus acidocaldarius maltopentaose + alpha,alpha'-trehalose
-
?

Subunits

Subunits Comment Organism
? x * 59000, SDS-PAGE Sulfolobus acidocaldarius

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
-
Sulfolobus acidocaldarius

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 90 40°C: about 45% of maximal activity, 90°C: about 65% of maximal activity Sulfolobus acidocaldarius

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
85
-
60 min, stable below Sulfolobus acidocaldarius

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6
-
Sulfolobus acidocaldarius

pH Range

pH Minimum pH Maximum Comment Organism
5 7 pH 5: about 80% of maximal activity, pH 7: about 65% of maximal activity Sulfolobus acidocaldarius

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 6 4°C, 24 h, stable Sulfolobus acidocaldarius