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Literature summary for 3.2.1.14 extracted from
- Biochemical characterization of a GH19 chitinase from Streptomyces alfalfae and its applications in crystalline chitin conversion and biocontrol (2021), Int. J. Biol. Macromol., 167, 193-201.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | 1 mM, 118.81% of initial activity | Streptomyces alfalfae |  |
| EDTA | 1 mM, 103.81% of initial activity | Streptomyces alfalfae | |
Application
| Application | Comment | Organism |
|---|---|---|
| degradation | in combination with N-acetyl hexosaminidase SaHEX, the enzyme yields a conversion rate of 95.2% from chitin powder to N-acetyl-D-glucosamine in 8 h and a product purity of >98.5% | Streptomyces alfalfae |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Streptomyces alfalfae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AgCl | 1 mM, 36.45% of initial activity | Streptomyces alfalfae | |
| CuCl2 | 1 mM, 70.95% of initial activity | Streptomyces alfalfae | |
| HgCl2 | 1 mM, no activity | Streptomyces alfalfae | |
| MnCl2 | 1 mM, 91.67% of initial activity | Streptomyces alfalfae | |
| NaCl | 1 mM, 89.05% of initial activity | Streptomyces alfalfae | |
| NiCl2 | 1 mM, 69.04% of initial activity | Streptomyces alfalfae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | 1 mM, 116.67% of initial activity | Streptomyces alfalfae | |
| CoCl2 | 1 mM, 184.13% of initial activity | Streptomyces alfalfae | |
| FeCl2 | 1 mM, 125.76% of initial activity | Streptomyces alfalfae | |
| FeCl3 | 1 mM, 115.43% of initial activity | Streptomyces alfalfae | |
| KCl | 1 mM, 107.86% of initial activity | Streptomyces alfalfae | |
| MgCl2 | 1 mM, 110.52% of initial activity | Streptomyces alfalfae | |
| ZnCl2 | 1 mM, 111.91% of initial activity | Streptomyces alfalfae | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces alfalfae | - |
- |
- |
| Streptomyces alfalfae ACCC 40021 | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8.7 | - |
substrate beta chitin, pH 8, 45°C | Streptomyces alfalfae |
| 28.4 | - |
substrate colloidal chitin, pH 8, 45°C | Streptomyces alfalfae |
| 286.6 | - |
substrate glycol chitin, pH 8, 45°C | Streptomyces alfalfae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-chitin + H2O | - |
Streptomyces alfalfae | ? | - |
? | |
| beta-chitin + H2O | - |
Streptomyces alfalfae ACCC 40021 | ? | - |
? | |
| chitooligosaccharides + H2O | degree of polymerization 3-6 | Streptomyces alfalfae | ? | - |
? | |
| chitooligosaccharides + H2O | degree of polymerization 3-6 | Streptomyces alfalfae ACCC 40021 | ? | - |
? | |
| colloidal chitin + H2O | - |
Streptomyces alfalfae | ? | - |
? | |
| colloidal chitin + H2O | - |
Streptomyces alfalfae ACCC 40021 | ? | - |
? | |
| glycol chitin + H2O | best substrate | Streptomyces alfalfae | ? | - |
? | |
| glycol chitin + H2O | best substrate | Streptomyces alfalfae ACCC 40021 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x* 29000, calculated from sequence, x * 33000, SDS-PAGE of recombinant protein | Streptomyces alfalfae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| A7J05_RS04860 | locus name | Streptomyces alfalfae |
| ChiB | - |
Streptomyces alfalfae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Streptomyces alfalfae |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 60 | - |
Streptomyces alfalfae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
2 h, 90% residual activity | Streptomyces alfalfae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Streptomyces alfalfae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 11 | - |
Streptomyces alfalfae |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Streptomyces alfalfae | calculated from sequence | - |
6.9 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme strongly inhibits the growth of tested pathogenic fungi | Streptomyces alfalfae |
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