Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-CodonPlus (DE3) RIL cells | Drosera rotundifolia |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
apoplast | - |
Drosera rotundifolia | 48046 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosera rotundifolia | A0A140GE99 | carnivorous sundew, cultivar Vikilan Jaervi | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography, and Superdex 200 gel filtration | Drosera rotundifolia |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | high expression | Drosera rotundifolia | - |
root | - |
Drosera rotundifolia | - |
stem | - |
Drosera rotundifolia | - |
trapping leaf | highest expression in tentacles | Drosera rotundifolia | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity with 4-methylumbelliferyl beta-D-N,N',N''-triacetylchitotrioside, 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside, and glycol chitin | Drosera rotundifolia | ? | - |
? | |
N-fluorescein-labeled chitin + H2O | - |
Drosera rotundifolia | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 32000, SDS-PAGE | Drosera rotundifolia |
? | x * 31900, calculated from amino acid sequence | Drosera rotundifolia |
? | x * 50000, recombinant enzyme fused to thioredoxin, SDS-PAGE | Drosera rotundifolia |
Synonyms | Comment | Organism |
---|---|---|
Chit | - |
Drosera rotundifolia |
endochitinase | - |
Drosera rotundifolia |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Drosera rotundifolia | calculated from amino acid sequence | - |
7.5 |
Organism | Comment | Expression |
---|---|---|
Drosera rotundifolia | enzyme expression is up-regulated by chitin by 7fold followed by sand (2.5fold) and gelatine (2fold), compared with expression in the non-treated (control) leaves | up |