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Literature summary for 3.2.1.14 extracted from

  • Feng, Y.; Kitaoku, Y.; Tanaka, J.; Taira, T.; Ohnuma, T.; Aachmann, F.L.; Fukamizo, T.
    Mode of action and specificity of a chitinase from unicellular microalgae, Euglena gracilis (2018), Plant Mol. Biol., 97, 553-564 .
    View publication on PubMed
Search for more literature for 3.2.1.14

Cloned(Commentary)

Cloned (Comment) Organism
catalytic domain Cat2 is expressed in Escherichia coli Rosetta-gami (DE3) cells Euglena gracilis

Organism

Organism UniProt Comment Textmining
Euglena gracilis
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-
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Purification (Commentary)

Purification (Comment) Organism
HiTrap Q column chromatography and Sephacryl S-100 gel filtration Euglena gracilis

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-N,N',N'',N''',N'''',N'''''-hexacetylchitohexaose + H2O
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 Euglena gracilis beta-D-N,N',N''-triacetylchitotriose + beta-D-N,N'-diacetylchitobiose + beta-D-N,N',N'',N'''-tetraacetylchitotetraose
-
 ?
beta-D-N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
-
 Euglena gracilis beta-D-N,N',N''-triacetylchitotriose + beta-D-N,N'-diacetylchitobiose
-
 ?
beta-D-N,N',N'',N'''-tetraacetylchitotetraose + H2O
-
 Euglena gracilis beta-D-N,N'-diacetylchitobiose + beta-D-N,N',N''-triacetylchitotriose + N-acetyl-beta-D-glucosamine
-
 ?
chitosan + H2O
-
 Euglena gracilis ?
-
 ?
additional information the enzyme acts toward chitin polysaccharide chain through a cooperative action of the two endo-splitting catalytic domains, recognizing two contiguous A-units at subsites -2 and -1 Euglena gracilis ?
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 ?