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Literature summary for 3.2.1.14 extracted from

  • Chen, W.; Qu, M.; Zhou, Y.; Yang, Q.
    Structural analysis of group II chitinase (ChtII) catalysis completes the puzzle of chitin hydrolysis in insects (2018), J. Biol. Chem., 293, 2652-2660 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.14

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris strain GS115 Ostrinia furnacalis

Crystallization (Commentary)

Crystallization (Comment) Organism
catalytically active domains ChtII-C1 and ChtII-C2, both in unliganded form and complexed with chitooligosaccharide substrates, hanging drop vapor diffusion method, using 0.2 M sodium chloride, 0.1 M Tris, pH 8.5, and 25% (w/v) PEG3350 for ChtII-C1 and 0.2 M ammonium acetate, 0.1 M Bis-Tris, pH 6.5, and 45% (w/v) 2-methyl-2,4-pentadiol for ChtII-C2 Ostrinia furnacalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00223
-
 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside domain ChtI, at pH 6.0 and 30°C Ostrinia furnacalis
0.01193
-
 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside domain ChtII-C2, at pH 6.0 and 30°C Ostrinia furnacalis
0.01527
-
 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside domain ChtII-C1C2, at pH 6.0 and 30°C Ostrinia furnacalis
0.01552
-
 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside domain ChtII-C1, at pH 6.0 and 30°C Ostrinia furnacalis

Organism

Organism UniProt Comment Textmining
Ostrinia furnacalis A0A221ZS22
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and HisTrap column chromatography Ostrinia furnacalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside + H2O
-
 Ostrinia furnacalis 4-methylumbelliferol + beta-D-N,N'-diacetylchitobioside
-
 ?
alpha-chitin + H2O
-
 Ostrinia furnacalis ?
-
 ?
beta-chitin + H2O
-
 Ostrinia furnacalis ?
-
 ?
colloidal chitin + H2O
-
 Ostrinia furnacalis ?
-
 ?

Synonyms

Synonyms Comment Organism
ChtII
-
 Ostrinia furnacalis
group II chitinase
-
 Ostrinia furnacalis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.2
-
 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside domain ChtII-C2, at pH 6.0 and 30°C Ostrinia furnacalis
0.55
-
 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside domain ChtII-C1, at pH 6.0 and 30°C Ostrinia furnacalis
0.78
-
 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside domain ChtII-C1C2, at pH 6.0 and 30°C Ostrinia furnacalis
1.78
-
 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside domain ChtI, at pH 6.0 and 30°C Ostrinia furnacalis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
17
-
 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside domain ChtII-C2, at pH 6.0 and 30°C Ostrinia furnacalis
35.5
-
 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside domain ChtII-C1, at pH 6.0 and 30°C Ostrinia furnacalis
51
-
 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside domain ChtII-C1C2, at pH 6.0 and 30°C Ostrinia furnacalis
800
-
 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside domain ChtI, at pH 6.0 and 30°C Ostrinia furnacalis