Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Trichoderma harzianum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3152 | - |
4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside | at pH 4.0 and 50°C | Trichoderma harzianum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitin + H2O | Trichoderma harzianum | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichoderma harzianum | A0A172Q4D8 | isolate-HP3 | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Trichoderma harzianum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Trichoderma harzianum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + H2O | - |
Trichoderma harzianum | 4-nitrophenyl beta-D-N,N'-diacetylchitobioside + 4-nitrophenyl N-acetylglucosaminide + beta-D-N,N'-diacetylchitobioside + N-acetylglucosamine | - |
? | |
chitin + H2O | - |
Trichoderma harzianum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 46370, calculated from amino acid sequence | Trichoderma harzianum |
? | x * 49400, recombinant His6-tagged enzyme, SDS-PAGE | Trichoderma harzianum |
Synonyms | Comment | Organism |
---|---|---|
ech46 | - |
Trichoderma harzianum |
endochitinase | - |
Trichoderma harzianum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Trichoderma harzianum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.107 | - |
4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside | at pH 4.0 and 50°C | Trichoderma harzianum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
- |
Trichoderma harzianum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Trichoderma harzianum | calculated from amino acid sequence | - |
5.6 |
Trichoderma harzianum | recombinant His6-tagged enzyme, isoelectric focusing | - |
6.7 |