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Literature summary for 3.2.1.14 extracted from

  • Sharma, V.; Salwan, R.; Sharma, P.N.; Kanwar, S.S.
    Molecular cloning and characterization of ech46 endochitinase from Trichoderma harzianum (2016), Int. J. Biol. Macromol., 92, 615-624 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Trichoderma harzianum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3152
-
4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside at pH 4.0 and 50°C Trichoderma harzianum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chitin + H2O Trichoderma harzianum
-
?
-
?

Organism

Organism UniProt Comment Textmining
Trichoderma harzianum A0A172Q4D8 isolate-HP3
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Trichoderma harzianum

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Trichoderma harzianum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + H2O
-
Trichoderma harzianum 4-nitrophenyl beta-D-N,N'-diacetylchitobioside + 4-nitrophenyl N-acetylglucosaminide + beta-D-N,N'-diacetylchitobioside + N-acetylglucosamine
-
?
chitin + H2O
-
Trichoderma harzianum ?
-
?

Subunits

Subunits Comment Organism
? x * 46370, calculated from amino acid sequence Trichoderma harzianum
? x * 49400, recombinant His6-tagged enzyme, SDS-PAGE Trichoderma harzianum

Synonyms

Synonyms Comment Organism
ech46
-
Trichoderma harzianum
endochitinase
-
Trichoderma harzianum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Trichoderma harzianum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.107
-
4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside at pH 4.0 and 50°C Trichoderma harzianum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
Trichoderma harzianum

pI Value

Organism Comment pI Value Maximum pI Value
Trichoderma harzianum calculated from amino acid sequence
-
5.6
Trichoderma harzianum recombinant His6-tagged enzyme, isoelectric focusing
-
6.7