Literature summary for 3.2.1.14 extracted from

Bouacem, K.; Laribi-Habchi, H.; Mechri, S.; Hacene, H.; Jaouadi, B.; Bouanane-Darenfed, A.
Biochemical characterization of a novel thermostable chitinase from Hydrogenophilus hirschii strain KB-DZ44 (2018), Int. J. Biol. Macromol., 106, 338-350 .

Search for more literature for 3.2.1.14

Activating Compound

Activating Compound	Comment	Organism	Structure
1-ethyl-3-(3-dimethylaminopropyl) carbodiimide	118% activity at 2 mM	Hydrogenophilus hirschii
1-Hexanol	about 140% activity at 25% (v/v)	Hydrogenophilus hirschii
ethanol	about 127% activity at 25% (v/v)	Hydrogenophilus hirschii
methanol	about 125% activity at 25% (v/v)	Hydrogenophilus hirschii
n-heptane	about 120% activity at 25% (v/v)	Hydrogenophilus hirschii
n-hexane	about 130% activity at 25% (v/v)	Hydrogenophilus hirschii

Inhibitors

Inhibitors	Comment	Organism	Structure
1-butanol	about 80% residual activity at 25% (v/v)	Hydrogenophilus hirschii
2-mercaptoethanol	about 10% residual activity at 5 mM	Hydrogenophilus hirschii
4-chloromercuribenzoic acid	complete inhibition at 2 mM	Hydrogenophilus hirschii
5,5'-dithiobis-(2-nitrobenzoic acid)	about 8% residual activity at 2 mM	Hydrogenophilus hirschii
acetonitrile	complete inhibition at 25% (v/v)	Hydrogenophilus hirschii
Ag+	88% inhibition at 2 mM	Hydrogenophilus hirschii
Al3+	39% inhibition at 2 mM	Hydrogenophilus hirschii
Ba2+	24% inhibition at 2 mM	Hydrogenophilus hirschii
Cd2+	complete inhibition at 2 mM	Hydrogenophilus hirschii
dithiothreitol	about 5% residual activity at 1 mM	Hydrogenophilus hirschii
ethyl acetate	complete inhibition at 25% (v/v)	Hydrogenophilus hirschii
Fe2+	55% inhibition at 2 mM	Hydrogenophilus hirschii
Hg2+	complete inhibition at 2 mM	Hydrogenophilus hirschii
isooctane	about 80% residual activity at 25% (v/v)	Hydrogenophilus hirschii
additional information	not significantly inhibited by 2,4,6-trinitrobenzenesulfonic acid, phenylmethylsulfonyl fluoride, diethyldicarbonate, N-bromosuccinimide, and N-acetylimidazole	Hydrogenophilus hirschii
N-ethylmaleimide	complete inhibition at 2 mM	Hydrogenophilus hirschii
Ni2+	complete inhibition at 2 mM	Hydrogenophilus hirschii
Zn2+	10% inhibition at 2 mM	Hydrogenophilus hirschii

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	130% activity at 2 mM	Hydrogenophilus hirschii
Co2+	105% activity at 2 mM	Hydrogenophilus hirschii
Cu2+	111% activity at 2 mM	Hydrogenophilus hirschii
Mg2+	305% activity at 2 mM	Hydrogenophilus hirschii
Mn2+	197% activity at 2 mM	Hydrogenophilus hirschii
additional information	K+ and Li+ do not show significant inhibition or activation effects	Hydrogenophilus hirschii

Organism

Organism	UniProt	Comment	Textmining
Hydrogenophilus hirschii	-	-	-
Hydrogenophilus hirschii KB-DZ44	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate precipitation, acetone precipitation, Sephacryl S-200 gel filtration and Mono Q Sepharose column chromatography	Hydrogenophilus hirschii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl beta-D-N,N',N'',N''',N''''-pentaacetylchitopentaoside + H2O	700% activity compared to colloidal chitin	Hydrogenophilus hirschii	4-nitrophenyl beta-D-N,N',N'',N'''-tetraacetylchitotetraoside + 4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + 4-nitrophenyl beta-D-N,N'-diacetylchitobioside + 4-nitrophenyl N-acetyl-beta-D-glucosamine	-	?
4-nitrophenyl beta-D-N,N',N'',N'''-tetraacetylchitotetraoside + H2O	250% activity compared to colloidal chitin	Hydrogenophilus hirschii	4-nitrophenyl beta-D-N,N'-diacetylchitobioside	-	?
4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + H2O	185% activity compared to colloidal chitin	Hydrogenophilus hirschii	?	-	?
chitin azure + H2O	98% activity compared to colloidal chitin	Hydrogenophilus hirschii	?	-	?
chitin azure + H2O	98% activity compared to colloidal chitin	Hydrogenophilus hirschii KB-DZ44	?	-	?
chitooligosaccharide + H2O	76% activity compared to colloidal chitin	Hydrogenophilus hirschii	?	-	?
chitotriose + H2O	85% activity compared to colloidal chitin	Hydrogenophilus hirschii	?	-	?
colloidal chitin + H2O	100% activity	Hydrogenophilus hirschii	?	-	?
glycol chitin + H2O	95% activity compared to colloidal chitin	Hydrogenophilus hirschii	?	-	?
glycol chitin + H2O	95% activity compared to colloidal chitin	Hydrogenophilus hirschii KB-DZ44	?	-	?
glycol chitosan + H2O	90% activity compared to colloidal chitin	Hydrogenophilus hirschii	?	-	?
glycol chitosan + H2O	90% activity compared to colloidal chitin	Hydrogenophilus hirschii KB-DZ44	?	-	?
additional information	the enzyme does not hydrolyze beta-D-N,N'-diacetylchitobioside, carboxymethyl cellulose, chitobiose, laminarin, and amylose	Hydrogenophilus hirschii	?	-	?
additional information	the enzyme does not hydrolyze beta-D-N,N'-diacetylchitobioside, carboxymethyl cellulose, chitobiose, laminarin, and amylose	Hydrogenophilus hirschii KB-DZ44	?	-	?
xylan + H2O	46% activity compared to colloidal chitin	Hydrogenophilus hirschii	?	-	?
xylan + H2O	46% activity compared to colloidal chitin	Hydrogenophilus hirschii KB-DZ44	?	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 59000, SDS-PAGE	Hydrogenophilus hirschii
monomer	1 * 59103, MALDI-TOF mass spectrometry	Hydrogenophilus hirschii

Synonyms

Synonyms	Comment	Organism
ChiA-Hh59	-	Hydrogenophilus hirschii
endo-chitinase	-	Hydrogenophilus hirschii

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
85	-	-	Hydrogenophilus hirschii

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
70	100	the half-life times at 70, 80, 90, and 100°C in the absence of Mg2+ are 8, 5, 3, and 1 h, respectively. In the presence of 2 mM Mg2+, the half-life times of the enzyme increase to 10, 7, 4, and 2 h, respectively	Hydrogenophilus hirschii

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
14400	-	colloidal chitin	at pH 5.0 and 70°C	Hydrogenophilus hirschii

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5	-	-	Hydrogenophilus hirschii

pH Range

pH Minimum	pH Maximum	Comment	Organism
3	9	the relative activities at pH 3.0 and 9.0 are 80 and 45%, respectively	Hydrogenophilus hirschii

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
3	5	the half-life times of the enzyme at pH 3.0, 4.0, and 5.0 are 10, 8, and 6 h, respectively	Hydrogenophilus hirschii

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Release 2023.1 (January 2023)