Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) CodonPlus cells | Thermobifida fusca |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
colloidal chitin | substrate inhibition | Thermobifida fusca |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermobifida fusca | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography and Superdex 75 gel filtration | Thermobifida fusca |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-chitin + H2O | - |
Thermobifida fusca | N,N',N''-triacetyl-beta-D-chitotriose + N,N'-diacetyl-beta-D-chitobiose | - |
? | |
beta-chitin + H2O | - |
Thermobifida fusca | ? | - |
? | |
chitohexaose + H2O | - |
Thermobifida fusca | chitobiose + chitotetraose + chitotriose | - |
? | |
additional information | activity towards colloidal chitin and acetylated chitosan is minimal. The enzyme does not use N,N',N''-triacetyl-beta-D-chitotriose and RNase B as substrates | Thermobifida fusca | ? | - |
? | |
N,N',N'',N''',N'''',N'''''-hexaacetyl-beta-D-chitohexaose + H2O | - |
Thermobifida fusca | N,N',N''-triacetyl-beta-D-chitotriose + N,N'-diacetyl-beta-D-chitobiose + N,N',N'',N'''-tetraacetyl-beta-D-chitotetraose | - |
? | |
N,N',N'',N''',N''''-pentaacetyl-beta-D-chitopentaose + H2O | - |
Thermobifida fusca | N,N',N''-triacetyl-beta-D-chitotriose + N,N'-diacetyl-beta-D-chitobiose | - |
? | |
N,N',N'',N'''-tetraacetyl-beta-D-chitotetraose + H2O | - |
Thermobifida fusca | 2 N,N'-diacetyl-beta-D-chitobiose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Chi18A | - |
Thermobifida fusca |
GH18 chitinase | - |
Thermobifida fusca |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 45 | at pH 6.5. At temperatures above 50 °C activity becomes drastically reduced | Thermobifida fusca |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
57.5 | - |
melting temperature | Thermobifida fusca |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | - |
Thermobifida fusca |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 7.8 | the enzyme displays more than 50% of maximum activity in the pH range of 6.0 to 7.8 | Thermobifida fusca |