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Literature summary for 3.2.1.14 extracted from

  • Gaber, Y.; Mekasha, S.; Vaaje-Kolstad, G.; Eijsink, V.G.H.; Fraaije, M.W.
    Characterization of a chitinase from the cellulolytic actinomycete Thermobifida fusca (2016), Biochim. Biophys. Acta, 1864, 1253-1259 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) CodonPlus cells Thermobifida fusca

Inhibitors

Inhibitors Comment Organism Structure
colloidal chitin substrate inhibition Thermobifida fusca

Organism

Organism UniProt Comment Textmining
Thermobifida fusca
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and Superdex 75 gel filtration Thermobifida fusca

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-chitin + H2O
-
Thermobifida fusca N,N',N''-triacetyl-beta-D-chitotriose + N,N'-diacetyl-beta-D-chitobiose
-
?
beta-chitin + H2O
-
Thermobifida fusca ?
-
?
chitohexaose + H2O
-
Thermobifida fusca chitobiose + chitotetraose + chitotriose
-
?
additional information activity towards colloidal chitin and acetylated chitosan is minimal. The enzyme does not use N,N',N''-triacetyl-beta-D-chitotriose and RNase B as substrates Thermobifida fusca ?
-
?
N,N',N'',N''',N'''',N'''''-hexaacetyl-beta-D-chitohexaose + H2O
-
Thermobifida fusca N,N',N''-triacetyl-beta-D-chitotriose + N,N'-diacetyl-beta-D-chitobiose + N,N',N'',N'''-tetraacetyl-beta-D-chitotetraose
-
?
N,N',N'',N''',N''''-pentaacetyl-beta-D-chitopentaose + H2O
-
Thermobifida fusca N,N',N''-triacetyl-beta-D-chitotriose + N,N'-diacetyl-beta-D-chitobiose
-
?
N,N',N'',N'''-tetraacetyl-beta-D-chitotetraose + H2O
-
Thermobifida fusca 2 N,N'-diacetyl-beta-D-chitobiose
-
?

Synonyms

Synonyms Comment Organism
Chi18A
-
Thermobifida fusca
GH18 chitinase
-
Thermobifida fusca

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 45 at pH 6.5. At temperatures above 50 °C activity becomes drastically reduced Thermobifida fusca

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
57.5
-
melting temperature Thermobifida fusca

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
Thermobifida fusca

pH Range

pH Minimum pH Maximum Comment Organism
6 7.8 the enzyme displays more than 50% of maximum activity in the pH range of 6.0 to 7.8 Thermobifida fusca