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Literature summary for 3.2.1.14 extracted from
- The deduced role of a chitinase containing two nonsynergistic catalytic domains (2018), Acta Crystallogr. Sect. D, 74, 30-40 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Pichia pastoris GS115 cells | Ostrinia furnacalis |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging drop vapor diffusion method, using 200 mM ammonium sulfate, 100 mM bis-Tris pH 6.5, 20% (w/v) PEG 3350 for domain GH18A, and 200 mM trisodium citrate dihydrate pH 8.1, 20% (w/v) PEG 3350 for domain GH18B | Ostrinia furnacalis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E217L | domain GH18A active site mutant | Ostrinia furnacalis |
| E647L | domain GH18B active site mutant | Ostrinia furnacalis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| single-chained chitin + H2O | Ostrinia furnacalis | - |
? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ostrinia furnacalis | U5LUV7 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HisTrap column chromatography and Resource S column chromatography | Ostrinia furnacalis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cuticle | - |
Ostrinia furnacalis | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ethylene glycol chitin + H2O | - |
Ostrinia furnacalis | ? | - |
? | |
| hexa-N-acetyl-chitohexaose + H2O | highest activity | Ostrinia furnacalis | 2 tri-N-acetyl-chitotriose | - |
? | |
| additional information | the enzyme is inactive towards tri-N-acetyl-chitotriose and insoluble chitin substrates like alpha-chitin, beta-chitin, and colloidal chitin | Ostrinia furnacalis | ? | - |
? | |
| penta-N-acetylchitopentaose + H2O | - |
Ostrinia furnacalis | ? | - |
? | |
| single-chained chitin + H2O | - |
Ostrinia furnacalis | ? | - |
? | |
| tetra-N-acetyl-chitotetraose + H2O | - |
Ostrinia furnacalis | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ChtIII | - |
Ostrinia furnacalis |
| group III chitinase | - |
Ostrinia furnacalis |
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Release 2023.1 (January 2023)
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