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Literature summary for 3.2.1.14 extracted from

  • Ueda, J.; Kurosawa, N.
    Characterization of an extracellular thermophilic chitinase from Paenibacillus thermoaerophilus strain TC22-2b isolated from compost (2015), World J. Microbiol. Biotechnol., 31, 135-143.
    View publication on PubMed

Application

Application Comment Organism
synthesis the thermophilic chitinase may prove useful for industrial applications in chitooligosaccharide production from chitin Paenibacillus thermoaerophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4
-
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside pH 7.0, 60°C Paenibacillus thermoaerophilus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Paenibacillus thermoaerophilus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
x * 48000, SDS-PAGE Paenibacillus thermoaerophilus

Organism

Organism UniProt Comment Textmining
Paenibacillus thermoaerophilus
-
isolated from compost (55°C) in Japan
-
Paenibacillus thermoaerophilus TC22-2b
-
isolated from compost (55°C) in Japan
-

Purification (Commentary)

Purification (Comment) Organism
native extracellular enzyme 4.5fold from cell culture medium by ammonium sulfate fractionation, colloidal chitin adsorption, and ion exchange chromatography Paenibacillus thermoaerophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.32
-
purified enzyme, substrate 4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside, pH 7.0, 50°C Paenibacillus thermoaerophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O
-
Paenibacillus thermoaerophilus 4-nitrophenol + N,N'-diacetyl-beta-D-chitobiose
-
?
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O
-
Paenibacillus thermoaerophilus TC22-2b 4-nitrophenol + N,N'-diacetyl-beta-D-chitobiose
-
?
colloidal chitin + H2O prepared from commercial chitin Paenibacillus thermoaerophilus N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose + N,N',N'',N'''-tetraacetylchitotetraose
-
?
colloidal chitin + H2O prepared from commercial chitin Paenibacillus thermoaerophilus TC22-2b N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose + N,N',N'',N'''-tetraacetylchitotetraose
-
?
additional information the chitinase digests N-acetyl-chitooligosaccharides in an endo manner. N-acetylglucosamine dimers are not degraded by the enzyme Paenibacillus thermoaerophilus ?
-
?
additional information the chitinase digests N-acetyl-chitooligosaccharides in an endo manner. N-acetylglucosamine dimers are not degraded by the enzyme Paenibacillus thermoaerophilus TC22-2b ?
-
?

Subunits

Subunits Comment Organism
? x * 48000, SDS-PAGE Paenibacillus thermoaerophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Paenibacillus thermoaerophilus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70 activity range Paenibacillus thermoaerophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
the purified chitinase retains 68% of its initial activity after 2 h at 50°C Paenibacillus thermoaerophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
9.6
-
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside pH 7.0, 60°C Paenibacillus thermoaerophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
Paenibacillus thermoaerophilus

pH Range

pH Minimum pH Maximum Comment Organism
4 7 50% of maximal activity within this range Paenibacillus thermoaerophilus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3
-
purified enzyme, 30°C, 40% activity remaining after 2 h Paenibacillus thermoaerophilus
4 10 purified enzyme, 30°C, 80% activity remaining after 2 h Paenibacillus thermoaerophilus