Application | Comment | Organism |
---|---|---|
synthesis | the thermophilic chitinase may prove useful for industrial applications in chitooligosaccharide production from chitin | Paenibacillus thermoaerophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.4 | - |
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside | pH 7.0, 60°C | Paenibacillus thermoaerophilus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Paenibacillus thermoaerophilus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
x * 48000, SDS-PAGE | Paenibacillus thermoaerophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenibacillus thermoaerophilus | - |
isolated from compost (55°C) in Japan | - |
Paenibacillus thermoaerophilus TC22-2b | - |
isolated from compost (55°C) in Japan | - |
Purification (Comment) | Organism |
---|---|
native extracellular enzyme 4.5fold from cell culture medium by ammonium sulfate fractionation, colloidal chitin adsorption, and ion exchange chromatography | Paenibacillus thermoaerophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.32 | - |
purified enzyme, substrate 4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside, pH 7.0, 50°C | Paenibacillus thermoaerophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O | - |
Paenibacillus thermoaerophilus | 4-nitrophenol + N,N'-diacetyl-beta-D-chitobiose | - |
? | |
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O | - |
Paenibacillus thermoaerophilus TC22-2b | 4-nitrophenol + N,N'-diacetyl-beta-D-chitobiose | - |
? | |
colloidal chitin + H2O | prepared from commercial chitin | Paenibacillus thermoaerophilus | N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose + N,N',N'',N'''-tetraacetylchitotetraose | - |
? | |
colloidal chitin + H2O | prepared from commercial chitin | Paenibacillus thermoaerophilus TC22-2b | N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose + N,N',N'',N'''-tetraacetylchitotetraose | - |
? | |
additional information | the chitinase digests N-acetyl-chitooligosaccharides in an endo manner. N-acetylglucosamine dimers are not degraded by the enzyme | Paenibacillus thermoaerophilus | ? | - |
? | |
additional information | the chitinase digests N-acetyl-chitooligosaccharides in an endo manner. N-acetylglucosamine dimers are not degraded by the enzyme | Paenibacillus thermoaerophilus TC22-2b | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 48000, SDS-PAGE | Paenibacillus thermoaerophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Paenibacillus thermoaerophilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | activity range | Paenibacillus thermoaerophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
the purified chitinase retains 68% of its initial activity after 2 h at 50°C | Paenibacillus thermoaerophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.6 | - |
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside | pH 7.0, 60°C | Paenibacillus thermoaerophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
- |
Paenibacillus thermoaerophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 7 | 50% of maximal activity within this range | Paenibacillus thermoaerophilus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | - |
purified enzyme, 30°C, 40% activity remaining after 2 h | Paenibacillus thermoaerophilus |
4 | 10 | purified enzyme, 30°C, 80% activity remaining after 2 h | Paenibacillus thermoaerophilus |