Application | Comment | Organism |
---|---|---|
analysis | isozyme YlCts1p can be used as a good model for studying O-glycosylation in the dimorphic yeast Yersinia lipolytica | Yarrowia lipolytica |
Cloned (Comment) | Organism |
---|---|
gene YlCTS1, DNA and amino acid sequence determination, analysis and comparison | Yarrowia lipolytica |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the enzyme is a secreted glycoprotein modified with intensive O-mannosylation | Yarrowia lipolytica | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitin + H2O | Yarrowia lipolytica | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Yarrowia lipolytica | - |
gene YlCTS1 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the isozyme YlCts1p is modified only by O-mannosylation without N-glycosylation, deglycosylation by jack bean alpha-mannosidase digestion but not by PNGase F treatment | Yarrowia lipolytica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl beta-D-N,N',N''-triacetylchitotrioside + H2O | - |
Yarrowia lipolytica | 4-methylumbelliferol + beta-D-N,N',N''-triacetylchitotriose | - |
? | |
chitin + H2O | - |
Yarrowia lipolytica | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
endochitinase | - |
Yarrowia lipolytica |
YlCts1p | - |
Yarrowia lipolytica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
assay at | Yarrowia lipolytica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
assay at | Yarrowia lipolytica |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is a glycoside hydrolase family 18 chitinase | Yarrowia lipolytica |
malfunction | isozyme YlCts1p deletion results in remarkably reduced extracellular endochitinase activity in the culture supernatant of Yersinia lipolytica and enhanced cell aggregation | Yarrowia lipolytica |
additional information | isozyme YlCts1p contains an N-terminal secretion signal peptide, a long C-terminal Ser/Thr-rich domain, and a chitin-binding domain | Yarrowia lipolytica |
physiological function | chitinase cleaves the beta-1,4-linkage between N-acetyl-D-glucosamine residues in the linear homopolymer chitin, which is an essential component of yeast and fungal cell walls | Yarrowia lipolytica |