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Literature summary for 3.2.1.14 extracted from

  • Huang, L.; Shizume, A.; Nogawa, M.; Taguchi, G.; Shimosaka, M.
    Heterologous expression and functional characterization of a novel chitinase from the chitinolytic bacterium Chitiniphilus shinanonensis (2012), Biosci. Biotechnol. Biochem., 76, 517-522.
    View publication on PubMed
Search for more literature for 3.2.1.14

Cloned(Commentary)

Cloned (Comment) Organism
gene chiC, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha Chitiniphilus shinanonensis
gene chiD, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha Chitiniphilus shinanonensis
gene chiE, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha Chitiniphilus shinanonensis
gene chiF, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha Chitiniphilus shinanonensis
gene chiG, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha Chitiniphilus shinanonensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54400
-
 x * 54400, recombinant enzyme, SDS-PAGE Chitiniphilus shinanonensis
74400
-
 x * 74400, recombinant enzyme, SDS-PAGE Chitiniphilus shinanonensis
80800
-
 x * 80800, recombinant enzyme, SDS-PAGE Chitiniphilus shinanonensis

Organism

Organism UniProt Comment Textmining
Chitiniphilus shinanonensis F8WSQ0 gene chiC encoded in the chiCDEFG operon
-
Chitiniphilus shinanonensis F8WSQ1 gene chiD encoded in the chiCDEFG operon
-
Chitiniphilus shinanonensis F8WSQ2 gene chiE encoded in the chiCDEFG operon
-
Chitiniphilus shinanonensis F8WSQ3 gene chiF encoded in the chiCDEFG operon
-
Chitiniphilus shinanonensis F8WSQ4 gene chiG encoded in the chiCDEFG operon
-
Chitiniphilus shinanonensis SAY3 F8WSQ0 gene chiC encoded in the chiCDEFG operon
-
Chitiniphilus shinanonensis SAY3 F8WSQ1 gene chiD encoded in the chiCDEFG operon
-
Chitiniphilus shinanonensis SAY3 F8WSQ2 gene chiE encoded in the chiCDEFG operon
-
Chitiniphilus shinanonensis SAY3 F8WSQ3 gene chiF encoded in the chiCDEFG operon
-
Chitiniphilus shinanonensis SAY3 F8WSQ4 gene chiG encoded in the chiCDEFG operon
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by affinity chromatography Chitiniphilus shinanonensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-N,N',N''-triacetylchitotrioside + H2O
-
 Chitiniphilus shinanonensis ?
-
 ?
4-nitrophenyl beta-N,N',N''-triacetylchitotrioside + H2O
-
 Chitiniphilus shinanonensis SAY3 ?
-
 ?
4-nitrophenyl beta-N,N'-diacetylchitobioside + H2O
-
 Chitiniphilus shinanonensis ?
-
 ?
4-nitrophenyl beta-N,N'-diacetylchitobioside + H2O
-
 Chitiniphilus shinanonensis SAY3 ?
-
 ?
4-nitrophenyl beta-N,N'-diacetylchitobioside + H2O
-
 Chitiniphilus shinanonensis 4-nitrophenol + N,N'-diacetylchitobiose
-
 ?
4-nitrophenyl beta-N,N'-diacetylchitobioside + H2O
-
 Chitiniphilus shinanonensis SAY3 4-nitrophenol + N,N'-diacetylchitobiose
-
 ?
colloidal chitin + H2O from crab shell Chitiniphilus shinanonensis chitobiose + D-glucosamine
-
 ?
colloidal chitin + H2O from crab shell Chitiniphilus shinanonensis N-acetyl-beta-D-glucosamine + ? isozyme ChiG shows unique activity and completely splits the dimer into monomers ?
additional information no activity with colloidal chitin Chitiniphilus shinanonensis ?
-
 ?
additional information endochitinase that cleaves oligosaccharides with three or more N-acetyl-beta-D-glucosamine monomers, no activity with 4-nitrophenyl N-acetylglucosamine Chitiniphilus shinanonensis ?
-
 ?
additional information endochitinase that cleaves oligosacchrides with three or more N-acetyl-beta-D-glucosamine monomers, no activity with 4-nitrophenyl N-acetylglucosamine Chitiniphilus shinanonensis ?
-
 ?
additional information endochitinase, isozyme ChiG shows unique activity and completely splits the dimer into monomers, no activity with 4-nitrophenyl N-acetylglucosamine Chitiniphilus shinanonensis ?
-
 ?
additional information the recombinant isozyme ChiF has no chitinolytic activity Chitiniphilus shinanonensis ?
-
 ?
additional information endochitinase that cleaves oligosaccharides with three or more N-acetyl-beta-D-glucosamine monomers, no activity with 4-nitrophenyl N-acetylglucosamine Chitiniphilus shinanonensis SAY3 ?
-
 ?
additional information no activity with colloidal chitin Chitiniphilus shinanonensis SAY3 ?
-
 ?
additional information endochitinase that cleaves oligosacchrides with three or more N-acetyl-beta-D-glucosamine monomers, no activity with 4-nitrophenyl N-acetylglucosamine Chitiniphilus shinanonensis SAY3 ?
-
 ?
additional information the recombinant isozyme ChiF has no chitinolytic activity Chitiniphilus shinanonensis SAY3 ?
-
 ?
additional information endochitinase, isozyme ChiG shows unique activity and completely splits the dimer into monomers, no activity with 4-nitrophenyl N-acetylglucosamine Chitiniphilus shinanonensis SAY3 ?
-
 ?
N,N',N'',N''', N''''-pentaacetylchitopentaose + H2O
-
 Chitiniphilus shinanonensis 5 N-acetyl-D-glucosamine
-
 ?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
-
 Chitiniphilus shinanonensis N,N'-diacetylchitobiose + GlcNAc
-
 ?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
-
 Chitiniphilus shinanonensis SAY3 N,N'-diacetylchitobiose + GlcNAc
-
 ?
N,N',N'',N'''-tetraacetylchitotetraose + 3 H2O
-
 Chitiniphilus shinanonensis 4 N-acetyl-beta-D-glucosamine
-
 ?
N,N',N''-triacetylchitotriose + 2 H2O
-
 Chitiniphilus shinanonensis 3 N-acetyl-D-glucosamine
-
 ?
N,N',N''-triacetylchitotriose + 2 H2O
-
 Chitiniphilus shinanonensis SAY3 3 N-acetyl-D-glucosamine
-
 ?
N,N'-diacetylchitobiose + H2O
-
 Chitiniphilus shinanonensis 2 acetyl-D-glucosamine
-
 ?
partially N-acetylated chitosan + H2O
-
 Chitiniphilus shinanonensis ?
-
 ?
partially N-acetylated chitosan + H2O
-
 Chitiniphilus shinanonensis SAY3 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 54400, recombinant enzyme, SDS-PAGE Chitiniphilus shinanonensis
? x * 74400, recombinant enzyme, SDS-PAGE Chitiniphilus shinanonensis
? x * 80800, recombinant enzyme, SDS-PAGE Chitiniphilus shinanonensis
More isozyme domain structures, overview Chitiniphilus shinanonensis

Synonyms

Synonyms Comment Organism
ChiC
-
 Chitiniphilus shinanonensis
ChiD
-
 Chitiniphilus shinanonensis
ChiE
-
 Chitiniphilus shinanonensis
ChiF
-
 Chitiniphilus shinanonensis
ChiG
-
 Chitiniphilus shinanonensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Chitiniphilus shinanonensis
43
-
-
 Chitiniphilus shinanonensis
45
-
-
 Chitiniphilus shinanonensis
55
-
-
 Chitiniphilus shinanonensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Chitiniphilus shinanonensis

General Information

General Information Comment Organism
evolution the enzyme belongs to the glycosyl hydrolase family GH18 Chitiniphilus shinanonensis
evolution the enzymes of the operon chiCDEFG belong to the glycosyl hydrolase family GH18, while chiF encodes a polypeptide with two chitin-binding domains, but no catalytic domain Chitiniphilus shinanonensis
metabolism the isozymes encoded by chiCDEFG operon act in a cooperative manner in chitin degradation Chitiniphilus shinanonensis