Cloned (Comment) | Organism |
---|---|
expression of recombinant His-tagged Chi enzymes in Escherichia coli strain BL21(DE3)/pLysS | Francisella tularensis |
expression of recombinant His-tagged Chi enzymes in Escherichia coli strain BL21(DE3)/pLysS | Francisella tularensis subsp. novicida |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of chiA and chiC knockouts strains A1 and A2 strains, respectively | Francisella tularensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | comparative endochitinase kinetics from different strains, overview | Francisella tularensis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Francisella tularensis subsp. novicida | - |
- |
intracellular | - |
Francisella tularensis | 5622 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
79000 | - |
x * 79000, recombinant His-tagged ChiB, SDS-PAGE, x * 120000, recombinant His-tagged ChiA, SDS-PAGE, x * 97000, about, recombinant His-tagged ChiA, sequence calculation | Francisella tularensis subsp. novicida |
97000 | - |
x * 79000, recombinant His-tagged ChiB, SDS-PAGE, x * 120000, recombinant His-tagged ChiA, SDS-PAGE, x * 97000, about, recombinant His-tagged ChiA, sequence calculation | Francisella tularensis subsp. novicida |
120000 | - |
x * 79000, recombinant His-tagged ChiB, SDS-PAGE, x * 120000, recombinant His-tagged ChiA, SDS-PAGE, x * 97000, about, recombinant His-tagged ChiA, sequence calculation | Francisella tularensis subsp. novicida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Francisella tularensis | - |
several strains, genes chiA, chiB, chiC, and chiD | - |
Francisella tularensis subsp. novicida | - |
different strins, genes chiA, chiB, chiC, and chiD | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged Chi enzymes from Escherichia coli strain BL21(DE3)/pLysS by nickel affinity chromatography | Francisella tularensis |
recombinant His-tagged Chi enzymes from Escherichia coli strain BL21(DE3)/pLysS by nickel affinity chromatography | Francisella tularensis subsp. novicida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
chitinase isozyme activity in different strains, overview | Francisella tularensis |
additional information | - |
chitinase isozyme activity in different strains, overview | Francisella tularensis subsp. novicida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O | - |
Francisella tularensis | ? | - |
? | |
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O | - |
Francisella tularensis subsp. novicida | ? | - |
? | |
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O | - |
Francisella tularensis | 4-nitrophenol + N-acetyl-beta-D-glucosamine | - |
? | |
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O | - |
Francisella tularensis subsp. novicida | 4-nitrophenol + N-acetyl-beta-D-glucosamine | - |
? | |
4-nitrophenyl tri-N-acetylchitotrioside + H2O | - |
Francisella tularensis | 4-nitrophenol + tri-N-acetylchitotriose | - |
? | |
4-nitrophenyl tri-N-acetylchitotrioside + H2O | - |
Francisella tularensis subsp. novicida | 4-nitrophenol + tri-N-acetylchitotriose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 79000, recombinant His-tagged ChiB, SDS-PAGE, x * 120000, recombinant His-tagged ChiA, SDS-PAGE, x * 97000, about, recombinant His-tagged ChiA, sequence calculation | Francisella tularensis subsp. novicida |
Synonyms | Comment | Organism |
---|---|---|
ChiA | - |
Francisella tularensis |
ChiA | - |
Francisella tularensis subsp. novicida |
ChiB | - |
Francisella tularensis |
ChiB | - |
Francisella tularensis subsp. novicida |
ChiC | - |
Francisella tularensis |
ChiC | - |
Francisella tularensis subsp. novicida |
ChiD | - |
Francisella tularensis |
ChiD | - |
Francisella tularensis subsp. novicida |
endochitinase | - |
Francisella tularensis |
endochitinase | - |
Francisella tularensis subsp. novicida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
assay at | Francisella tularensis |
35 | - |
assay at | Francisella tularensis subsp. novicida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Francisella tularensis |
7.4 | - |
assay at | Francisella tularensis subsp. novicida |