Cloned (Comment) | Organism |
---|---|
gene chiB, DNA and amino acid sequence determination and analysis, expression of His-tagged Is-chiB in Escherichia coli strain BL21 (DE3), subcloning in Escherichia coli strain DH5alpha | Isoptericola jiangsuensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | inhibits slightly at 10 mM | Isoptericola jiangsuensis | |
Cr3+ | inhibits slightly at 10 mM | Isoptericola jiangsuensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is not affected by Ca2+, Ba2+, Zn2+, Li+, Mn2+, Mg2+, Cu2+, Fe3+, Pb2+, and Cd2+ | Isoptericola jiangsuensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59330 | - |
x * 60000, recombinant enzyme, SDS-PAGE, x * 59330, sequence calculation | Isoptericola jiangsuensis |
60000 | - |
x * 60000, recombinant enzyme, SDS-PAGE, x * 59330, sequence calculation | Isoptericola jiangsuensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Isoptericola jiangsuensis | D3YGV3 | DSM 21863, CCTCC AB208287, gene chiB encoding isozyme Is-chiB | - |
Isoptericola jiangsuensis CLG | D3YGV3 | DSM 21863, CCTCC AB208287, gene chiB encoding isozyme Is-chiB | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged Is-chiB from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography and ultrafiltration | Isoptericola jiangsuensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl N,N',N''-triacetylchitotriose + H2O | - |
Isoptericola jiangsuensis | ? | - |
? | |
4-methylumbelliferyl N,N',N''-triacetylchitotriose + H2O | - |
Isoptericola jiangsuensis CLG | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, recombinant enzyme, SDS-PAGE, x * 59330, sequence calculation | Isoptericola jiangsuensis |
Synonyms | Comment | Organism |
---|---|---|
endochitinase | - |
Isoptericola jiangsuensis |
Is-chiB | - |
Isoptericola jiangsuensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Isoptericola jiangsuensis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 90 | activity range, profile, overview | Isoptericola jiangsuensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
purified recombinant enzyme, stable below | Isoptericola jiangsuensis |
50 | - |
purified recombinant enzyme, 1 h, 30% activity remaining | Isoptericola jiangsuensis |
80 | - |
purified recombinant enzyme, 1 h, complete inactivation | Isoptericola jiangsuensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Isoptericola jiangsuensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 10 | activity range, profile, overview | Isoptericola jiangsuensis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | - |
purified recombinant enzyme, 1 h, about 5% activity remaining | Isoptericola jiangsuensis |
5 | - |
purified recombinant enzyme, 1 h, 50% activity remaining | Isoptericola jiangsuensis |
7 | 10 | purified recombinant enzyme, 1 h, over 80% activity remaining, most stable at pH 8.5 | Isoptericola jiangsuensis |