Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Bacillus sp. (in: Bacteria) | |
DTT | - |
Bacillus sp. (in: Bacteria) | |
EDTA | - |
Bacillus sp. (in: Bacteria) |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Bacillus sp. (in: Bacteria) | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates | Bacillus sp. (in: Bacteria) | |
Mg2+ | activates | Bacillus sp. (in: Bacteria) | |
additional information | no effect on enzyme activity by Na+, K+, and Fe3+ | Bacillus sp. (in: Bacteria) | |
Zn2+ | activates | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
808000 | - |
x * 808000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
isolated from a soil sample collected from hot spring of XinJiang Province, China | - |
Bacillus sp. (in: Bacteria) Hu1 | - |
isolated from a soil sample collected from hot spring of XinJiang Province, China | - |
Purification (Comment) | Organism |
---|---|
native extracellular enzyme 5.6fold by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration | Bacillus sp. (in: Bacteria) |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
62.4 | - |
purified enzyme, pH 6.5, 60°C | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
colloid chitin + H2O | best substrate | Bacillus sp. (in: Bacteria) | ? | - |
? | |
colloid chitin + H2O | best substrate | Bacillus sp. (in: Bacteria) Hu1 | ? | - |
? | |
additional information | the enzyme is not active on carboxymethyl cellulose, cellulose, and soluble starch, as well as on chitosan with a deacetylation degree of 80-95%. The chitinase has high specificity in hydrolyzing glycosidic bond between GlcNAc-GlcNAc | Bacillus sp. (in: Bacteria) | ? | - |
? | |
additional information | the enzyme is not active on carboxymethyl cellulose, cellulose, and soluble starch, as well as on chitosan with a deacetylation degree of 80-95%. The chitinase has high specificity in hydrolyzing glycosidic bond between GlcNAc-GlcNAc | Bacillus sp. (in: Bacteria) Hu1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 808000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 80 | activity range, profile, overview | Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
purified enzyme, 1.31fold activation of enzyme activity after 60 min, remaining activity after 100 min | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Bacillus sp. (in: Bacteria) |