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Literature summary for 3.2.1.14 extracted from

  • Dai, D.; Hu, W.; Huang, G.; Li, W.
    Purification and characterization of a novel extracellular chitinase from thermophilic Bacillus sp. Hu1 (2011), Afr. J. Biotechnol., 10, 2476-2485.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
Bacillus sp. (in: Bacteria)
DTT
-
Bacillus sp. (in: Bacteria)
EDTA
-
Bacillus sp. (in: Bacteria)

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Bacillus sp. (in: Bacteria)
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Bacillus sp. (in: Bacteria)
Mg2+ activates Bacillus sp. (in: Bacteria)
additional information no effect on enzyme activity by Na+, K+, and Fe3+ Bacillus sp. (in: Bacteria)
Zn2+ activates Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
808000
-
x * 808000, SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
isolated from a soil sample collected from hot spring of XinJiang Province, China
-
Bacillus sp. (in: Bacteria) Hu1
-
isolated from a soil sample collected from hot spring of XinJiang Province, China
-

Purification (Commentary)

Purification (Comment) Organism
native extracellular enzyme 5.6fold by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
62.4
-
purified enzyme, pH 6.5, 60°C Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
colloid chitin + H2O best substrate Bacillus sp. (in: Bacteria) ?
-
?
colloid chitin + H2O best substrate Bacillus sp. (in: Bacteria) Hu1 ?
-
?
additional information the enzyme is not active on carboxymethyl cellulose, cellulose, and soluble starch, as well as on chitosan with a deacetylation degree of 80-95%. The chitinase has high specificity in hydrolyzing glycosidic bond between GlcNAc-GlcNAc Bacillus sp. (in: Bacteria) ?
-
?
additional information the enzyme is not active on carboxymethyl cellulose, cellulose, and soluble starch, as well as on chitosan with a deacetylation degree of 80-95%. The chitinase has high specificity in hydrolyzing glycosidic bond between GlcNAc-GlcNAc Bacillus sp. (in: Bacteria) Hu1 ?
-
?

Subunits

Subunits Comment Organism
? x * 808000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Bacillus sp. (in: Bacteria)

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 80 activity range, profile, overview Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
purified enzyme, 1.31fold activation of enzyme activity after 60 min, remaining activity after 100 min Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Bacillus sp. (in: Bacteria)