Literature summary for 3.2.1.14 extracted from

Akrem, A.; Iqbal, S.; Buck, F.; Meyer, A.; Perbandt, M.; Voelter, W.; Betzel, C.
Isolation, purification, crystallization and preliminary crystallographic studies of a chitinase from Crocus vernus (2011), Acta Crystallogr. Sect. F, 67, 340-343.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
purified enzyme, hanging drop vapour diffusion method, 0.002 m of 16 mg/ml protein in 20 mM acetate, pH 5.0, is mixed with 0.002 ml of reservoir solution containing PEG 8000, equilibration over 1.0 ml reservoir solution, 20°C, 3 days, X-ray diffraction structure determination and analysis at 2.1 A resolution, molecular replacement	Crocus vernus

Organism

Organism	UniProt	Comment	Textmining
Crocus vernus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
native enzyme from seeds by cation exchange chromatography and gel filtration	Crocus vernus

Source Tissue

Source Tissue	Comment	Organism	Textmining
seed	-	Crocus vernus	-

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Release 2023.1 (January 2023)