Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.14 extracted from

  • Krykbaev, R.; Fitz, L.J.; Reddy, P.S.; Winkler, A.; Xuan, D.; Yang, X.; Fleming, M.; Wolf, S.F.
    Evolutionary and biochemical differences between human and monkey acidic mammalian chitinases (2010), Gene, 452, 63-71.
    View publication on PubMed
Search for more literature for 3.2.1.14

Inhibitors

Inhibitors Comment Organism Structure
methylallosamidin
-
 Homo sapiens
methylallosamidin
-
 Macaca fascicularis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
 4-methylumbelliferyl chitobioside pH 5.0, 25°C Macaca fascicularis
0.032
-
 4-methylumbelliferyl chitobioside pH 5.0, 25°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52200
-
 x * 52200, calculated Macaca fascicularis

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Macaca fascicularis D3GG13
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification sequence contains a hydrophobic leader sequence of 21 amino acids Macaca fascicularis

Source Tissue

Source Tissue Comment Organism Textmining
stomach high expression of isoform CHIA, no expression of pseudogene CHIA-L1 Homo sapiens
-
stomach high expression of isoform CHIA-L1, no expression of isoform CHIA Macaca fascicularis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl beta-N,N'-diacetylchitobioside + H2O
-
 Homo sapiens 4-methylumbelliferol + N,N'-diacetylchitobiose
-
 ?
4-methylumbelliferyl beta-N,N'-diacetylchitobioside + H2O
-
 Macaca fascicularis 4-methylumbelliferol + N,N'-diacetylchitobiose
-
 ?
4-methylumbelliferyl chitobioside + H2O
-
 Homo sapiens 4-methylumbelliferone + chitobiose
-
 ?
4-methylumbelliferyl chitobioside + H2O
-
 Macaca fascicularis 4-methylumbelliferone + chitobiose
-
 ?
carboxymethyl-substituted chitin labeled with Remazol Brilliant Violet + H2O
-
 Homo sapiens ?
-
 ?
carboxymethyl-substituted chitin labeled with Remazol Brilliant Violet + H2O
-
 Macaca fascicularis ?
-
 ?

Subunits

Subunits Comment Organism
? x * 52200, calculated Macaca fascicularis

Synonyms

Synonyms Comment Organism
MACase
-
 Macaca fascicularis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3
-
 4-methylumbelliferyl chitobioside pH 5.0, 25°C Homo sapiens
31.8
-
 4-methylumbelliferyl chitobioside pH 5.0, 25°C Macaca fascicularis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Homo sapiens
5
-
-
 Macaca fascicularis

pH Range

pH Minimum pH Maximum Comment Organism
2
-
 20% of maximum activity Macaca fascicularis
2
-
 30% of maximum activity Homo sapiens
3.5
-
 20% of maximum activity Macaca fascicularis
3.5
-
 30% of maximum activity Homo sapiens
6.5
-
 20% of maximum activity Macaca fascicularis
6.5
-
 30% of maximum activity Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0000057
-
 pH 5.0, 25°C Homo sapiens methylallosamidin
0.000054
-
 pH 5.0, 25°C Macaca fascicularis methylallosamidin

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.09
-
 4-methylumbelliferyl chitobioside pH 5.0, 25°C Homo sapiens
4.5
-
 4-methylumbelliferyl chitobioside pH 5.0, 25°C Macaca fascicularis